Abstract：
:This paper presents a methodology for the determination of reaction rate constants for nitrifying bacteria and their mean population percentage in biomass in an alternating oxidation ditch system. The method used is based on the growth rate equations of the ASM1 model (IWA) (Henze et al. in Activated sludge models ASM1, ASM2, ASM2d, and ASM3. IWA Scientific and Technical Report no. 9, IWA Publishing, London, UK, 2000) and the application of mass balance equations for nitrifiers and ammonium nitrogen in an operational cycle of the ditch system. The system consists of two ditches operating in four phases. Data from a large-scale oxidation ditch pilot plant with a total volume of 120 m(3) within an experimental period of 8 months was used. Maximum specific growth rate for autotrophs (μ(A)) and the half-saturation constant for ammonium nitrogen (K(NH)) were found to be 0.36 day(-1) and 0.65 mgNH(4)-N/l, respectively. Additionally, the average population percentage of the nitrifiers in the biomass was estimated to be around 3%.
journal_name
Bioprocess Biosyst Engjournal_title
Bioprocess and biosystems engineeringauthors
Mantziaras ID,Katsiri Adoi
10.1007/s00449-010-0446-2subject
Has Abstractpub_date
2011-01-01 00:00:00pages
57-65issue
1eissn
1615-7591issn
1615-7605journal_volume
34pub_type
杂志文章abstract：:Industrial grade soluble corn starch was used directly and effectively as the fermentation substrate for microbial exopolysaccharides production. Bacillus subtilis mutant strain NJ308 grew with untreated starch raw material as the sole carbon source. The real-time PCR results demonstrated that up-regulated genes encod...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-018-1915-2
更新日期：2018-06-01 00:00:00
abstract：:Rice straw is a lignocellulosic biomass that constitutes a renewable organic substance and alternative source of energy; however, its structure confounds the liberation of monosaccharides. Pretreating rice straw using a TiO(2)/UV system facilitated its hydrolysis with Accellerase 1000(™), suggesting that hydroxyl radi...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-011-0589-9
更新日期：2012-01-01 00:00:00
abstract：:Long-term and excessive use of monocrotophos (MPs) pesticide leads to an accumulation of MPs residues in agricultural products. Electrochemical biosensor technology was developed as a simple and efficient method for detecting MPs. However, commercial acetylcholinesterase (AChE) sensors are not applied in practical MPs...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-019-02226-2
更新日期：2020-02-01 00:00:00
abstract：:The characterization of anodic microbial communities is of great importance in the study of microbial fuel cells (MFCs). These kinds of devices mainly require a high abundance of anode respiring bacteria (ARB) in the anode chamber for optimal performance. This study evaluated the effect of different enrichments of env...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-019-02074-0
更新日期：2019-05-01 00:00:00
abstract：:L-Lysine is an essential amino acid that can be produced by chemical processes from fossil raw materials, as well as by microbial fermentation, the latter being a more efficient and environmentally friendly procedure. In this work, the production process of L-lysine-HCl is studied using a systematic approach based on ...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-017-1766-2
更新日期：2017-07-01 00:00:00
abstract：:Bacillusfirmus strain 37 produces the cyclomaltodextrin glucanotransferase (CGTase) enzyme and CGTase produces cyclodextrins (CDs) through a starch cyclization reaction. The strategy for the cloning and expression of recombinant CGTase is a potentially viable alternative for the economically viable production of CGTas...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-018-02068-4
更新日期：2019-04-01 00:00:00
abstract：:The growth rate and desulfurization capacity accumulated by the cells during the growth of Pseudomonas putida KTH2 under different oxygen transfer conditions in a stirred and sparged tank bioreactor have been studied. Hydrodynamic conditions were changed using different agitation conditions. During the culture, severa...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-016-1536-6
更新日期：2016-04-01 00:00:00
abstract：:In response to sudden decrease in osmotic pressure, halophilic microorganisms secrete their accumulated osmolytes. This specific stress response, combined with physiochemical responses to the altered environment, influence the membrane properties and integrity of cells, with consequent effects on growth and yields in ...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-013-0957-8
更新日期：2013-12-01 00:00:00
abstract：:Broad-spectrum antibodies can effectively recognize substances with similar structures and have broad application prospects in field rapid detection. In this study, broad-spectrum antibodies (Abs) against organophosphorus pesticides (OPs) were used as sensitive recognition elements, which could effectively recognize m...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-020-02472-9
更新日期：2020-11-08 00:00:00
abstract：:The effect of a well-defined mild shear stress on hybridoma cell viability (HB-8852) in a serum-free culture medium has been analysed, and the role as shear protector of different concentrations of fetal bovine serum have been studied. Samples harvested from cultures in their late exponential growth phase, were subjec...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-008-0295-4
更新日期：2009-10-01 00:00:00
abstract：:The objective of this study was to evaluate ethanol production and bioadsorption with four red seaweeds, Gelidium amansii, Gracilaria verrucosa, Kappaphycus alvarezii and Eucheuma denticulatum. To produce ethanol, thermal acid hydrolysis, enzymatic saccharification and fermentation was carried out. After pretreatment,...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-016-1571-3
更新日期：2016-06-01 00:00:00
abstract：:Porphyridium cruentum is one of the most valued microalgae species able to produce both pigments and exopolysaccharides. Conventional liquid suspended cultivation in ponds and photobioreactors show its disadvantages in lower cultivation efficiency and higher stirring power consumption due to the high viscosity of the ...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-016-1676-8
更新日期：2017-01-01 00:00:00
abstract：:In this study, the silica nanoparticles surface was modified by carboxylic acid with different number of alkyl chain and two different lipases were immobilized on it to increase the glycerolysis activity of the immobilized lipases in organic solvent system. Silica nanoparticles of about 15 nm diameter were grafted wit...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-017-1852-5
更新日期：2018-01-01 00:00:00
abstract：:The paper describes a recombinant Schneider 2 (rS2) cell culture and protein expression in a bioreactor. S2 cells were transfected with a plasmid containing a fusion protein (human mu opioid receptor, hMOR, and green fluorescent protein, EGFP) under the control of inducible metallothionein promoter. A bioprocess in a ...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-005-0033-0
更新日期：2006-04-01 00:00:00
abstract：:The proteolysis of cheese whey with the aid of immobilized enzymes is an attractive alternative for this by-product of the dairy industry. Among some possible applications for whey protein hydrolysates, one may cite their use as protein source for individuals with reduced capacity of digestion, or with genetic metabol...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-008-0222-8
更新日期：2009-01-01 00:00:00
abstract：:In this paper we propose a methodology to determine the structure of the pseudo-stoichiometric coefficient matrix K in a mass balance-based model and to identify its coefficients from a set of available data. The first stage consists in estimating the number of reactions that must be taken into account to represent th...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-005-0407-3
更新日期：2005-08-01 00:00:00
abstract：:Exogenous enzymes are extraneous enzymes that are not intrinsic to the subject. The exogenous enzyme industry has been rapidly developing recently. Successful application of recombinant DNA amplification, high-efficiency expression, and immobilization technology to genetically engineered bacteria provides a rich sourc...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章,评审
doi：10.1007/s00449-020-02463-w
更新日期：2020-11-04 00:00:00
abstract：:The fungicide carbendazim is an ecotoxic agent affecting aquatic biota. Due to its suspected hormone-disrupting effects, it is considered a "priority hazard substance" by the Water Framework Directive of the European Commission, and its degradation is of major concern. In this work, a horizontal tubular biofilm reacto...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-016-1717-3
更新日期：2017-04-01 00:00:00
abstract：:The aim of this study was to investigate the efficacy of a hybrid biofilm pilot-scale treatment plant, designed with a novel configuration by the integration of a fixed-film system, to improve nitrogen removal. The pilot-scale system was established at a wastewater treatment plant in Istanbul and operated based on str...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-018-2042-9
更新日期：2019-03-01 00:00:00
abstract：:Bacillus subtilis TD6 was isolated from Takifugu rubripes, also known as puffer fish. Cellulase from this strain was partially purified by ammonium sulphate precipitation up to 80% saturation, entrapped in calcium alginate beads, and finally characterized using CMC as the substrate. For optimization, various parameter...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-011-0630-z
更新日期：2012-01-01 00:00:00
abstract：:Vitamin B12 is a crucial fine chemical that is widely used in the pharmaceutical, food and chemical industries, and its production solely dependents on microbial fermentation. We previously constructed an artificial vitamin B12 biosynthesis pathway in Escherichia coli, but the yield of the engineered strains was low. ...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-020-02355-z
更新日期：2020-10-01 00:00:00
abstract：:The use of fed-batch extractive fermentation can overcome inhibitory effects caused by the substrate and ethanol to the yeast cells, since it allows regulate the substrate concentration and remove the product as it is produced. The present study describes the modelling and experimental validation of ethanol production...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-020-02414-5
更新日期：2020-12-01 00:00:00
abstract：:The hydrolase (Thermobifida fusca hydrolase; TfH) from T. fusca was produced in Escherichia coli as fusion protein using the OmpA leader sequence and a His(6) tag. Productivity could be raised more than 100-fold. Both batch and fed-batch cultivations yield comparable cell specific productivities whereas volumetric pro...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-006-0069-9
更新日期：2006-08-01 00:00:00
abstract：:A serine alkaline protease from a newly isolated alkaliphilic Bacillus altitudinis GVC11 was purified and characterized. The enzyme was purified to homogeneity by acetone precipitation, DEAE-cellulose anion exchange chromatography with 7.03-fold increase in specific activity and 15.25% recovery. The molecular weight o...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-010-0483-x
更新日期：2011-05-01 00:00:00
abstract：:The aim of the present study was to investigate the feasibility of several food-processing wastes as support substrate for lipolytic enzymes production by the fungus Rhizopus oryzae under solid-state conditions. Different experiments were conducted to select the variables that allow obtaining high levels of lipolytic ...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-010-0416-8
更新日期：2010-10-01 00:00:00
abstract：:This work aimed to develop an optimal carbon source feeding strategy to achieve maximal production of heparosan as a precursor of bioengineered heparin by Escherichia coli K5. Glycerol gave higher heparosan titer and productivity compared to glucose. The maximum heparosan production (187 mg/L) and heparosan productivi...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-012-0708-2
更新日期：2012-09-01 00:00:00
abstract：:Lignocellulosic biomass can be considered as one of the largest sources for the production of renewable biofuels (bioethanol). It involves an enzymatic treatment capable of ensuring the depolymerization of cellulose into fermentable sugars, followed by the production of ethanol by appropriate bacteriological fermentat...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章,评审
doi：10.1007/s00449-020-02297-6
更新日期：2020-06-01 00:00:00
abstract：:Pretreatment method for rapeseed straw by sodium hydroxide was investigated for production of bioethanol and biobutanol. Various pretreatment parameters, including temperature, time, and sodium hydroxide concentration were optimized using a statistical method which is a central composite design of response surface met...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-011-0650-8
更新日期：2012-06-01 00:00:00
abstract：:Microalgae are considered as a promising biotechnological strategy to capture CO2 from biogas, producing biomass with valuable energetic compounds. This study has evaluated the biotechnological potential to fix CO2 from biogas and biomass production by Scenedesmus obliquus cultured in four different formulations of cu...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-017-1828-5
更新日期：2017-12-01 00:00:00
abstract：:Pullulanase was extracellularly produced with an engineered Escherichia coli with a combined strategy. When auto-induction instead of isopropyl β-D-1-thiogalactopyranoside (IPTG) induction method was implemented, we observed increased extracellular activity (4.2 U ml(-1)) and cell biomass (7.95 g DCW l(-1)). Subsequen...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-013-1026-z
更新日期：2014-04-01 00:00:00
