Abstract:
:The effect of a well-defined mild shear stress on hybridoma cell viability (HB-8852) in a serum-free culture medium has been analysed, and the role as shear protector of different concentrations of fetal bovine serum have been studied. Samples harvested from cultures in their late exponential growth phase, were subjected in a rheometer to a constant shear stress of 0.41 +/- 0.02 Pa, and the evolution of viable and total cell concentrations was determined and compared with static controls. A simple segregated kinetic model for the viable and dead cells was used to know the effect of serum concentration on the specific cell growth and death rate of the cells.
journal_name
Bioprocess Biosyst Engjournal_title
Bioprocess and biosystems engineeringauthors
Legazpi L,Laca A,Díaz Mdoi
10.1007/s00449-008-0295-4subject
Has Abstractpub_date
2009-10-01 00:00:00pages
717-22issue
6eissn
1615-7591issn
1615-7605journal_volume
32pub_type
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