Enzyme precipitate coatings of lipase on polymer nanofibers.

Abstract:

:Lipase (LP) was immobilized on electrospun and ethanol-dispersed polystyrene-poly(styrene-co-maleic anhydride) (PS-PSMA) nanofibers (EtOH-NF) in the form of enzyme precipitate coatings (EPCs). LP precipitate coatings (EPCs-LP) were prepared in a three-step process, consisting of covalent attachment, LP precipitation, and crosslinking of precipitated LPs onto the covalently attached LPs via glutaraldehyde treatment. The LP precipitation was performed by adding various concentrations of ammonium sulfate (20-50%, w/v). EPCs-LP improved the LP activity and stability when compared to covalently attached LPs (CA-LP) and the enzyme coatings of LPs (EC-LP) without the LP precipitation. For example, the use of 40% (w/v) ammonium sulfate resulted in EPC40-LP with the highest activity, which was 4.0 and 3.6 times higher than those of CA-LP and EC-LP, respectively. After 165-day incubation under rigorous shaking at 200 rpm, the residual activities of EPC50-LP were 0.5 μM/min mg of EtOH-NF, representing 113 and 75 times higher than those of CA-LP and EC-LP, respectively. When LP was partially purified via a simple ammonium sulfate precipitation and dialysis, both activities and stabilities of EC-LP and EPC-LP could be marginally improved. It is anticipated that the improved LP activity and stability in the form of EPCs would allow for their potential applications in various bioconversion processes such as biodiesel production and ibuprofen resolution.

journal_name

Bioprocess Biosyst Eng

authors

An HJ,Lee HJ,Jun SH,Hwang SY,Kim BC,Kim K,Lee KM,Oh MK,Kim J

doi

10.1007/s00449-011-0534-y

subject

Has Abstract

pub_date

2011-09-01 00:00:00

pages

841-7

issue

7

eissn

1615-7591

issn

1615-7605

journal_volume

34

pub_type

杂志文章
  • Comprehensive assessment of the L-lysine production process from fermentation of sugarcane molasses.

    abstract::L-Lysine is an essential amino acid that can be produced by chemical processes from fossil raw materials, as well as by microbial fermentation, the latter being a more efficient and environmentally friendly procedure. In this work, the production process of L-lysine-HCl is studied using a systematic approach based on ...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-017-1766-2

    authors: Anaya-Reza O,Lopez-Arenas T

    更新日期:2017-07-01 00:00:00

  • Quantitative analysis of hydrogen peroxide with special emphasis on biosensors.

    abstract::Determination of hydrogen peroxide (H2O2) has become essential in pharmaceutical, biological, clinical and environmental studies. The conventional detection methods of H2O2 such as colourimetry, titration, chromatography, spectrophotometry, fluorimetry, chemiluminescence have limited success, due to their poor selecti...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章,评审

    doi:10.1007/s00449-017-1878-8

    authors: Pundir CS,Deswal R,Narwal V

    更新日期:2018-03-01 00:00:00

  • Exploring the potential of halotolerant bacteria for biodegradation of polycyclic aromatic hydrocarbon.

    abstract::The present study aimed to determine the degradation and transformation of three-ring PAHs phenanthrene and anthracene by Cryptococcus sp. MR22 and Halomonas sp. BR04 under halophilic conditions. The growth progress of Cryptococcus sp. MR22 and Halomonas sp. BR04 on anthracene and phenanthrene was monitored by colony-...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-020-02415-4

    authors: Al Farraj DA,Hadibarata T,Yuniarto A,Alkufeidy RM,Alshammari MK,Syafiuddin A

    更新日期:2020-12-01 00:00:00

  • Efficient production of (R)-(-)-2-hydroxy-4-phenylbutyric acid by recombinant Pichia pastoris expressing engineered D-lactate dehydrogenase from Lactobacillus plantarum with a single-site mutation.

    abstract::(R)-2-hydroxy-4-phenylbutyric acid (R-HPBA) is a valuable intermediate for the synthesis of angiotensin-converting enzyme inhibitors. The asymmetric reduction of 2-oxo-4-phenylbutyric acid (OPBA) by oxidoreductases is an efficient approach for its synthesis. Here, we report a novel biocatalytic approach for asymmetric...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-018-1965-5

    authors: Wang X,Yu Z,Tang J,Yi D,Chen S

    更新日期:2018-09-01 00:00:00

  • Biotreatment of acidic zinc- and copper-containing wastewater using ethanol-fed sulfidogenic anaerobic baffled reactor.

    abstract::The treatment of acidic (pH 6.5-3), sulfate- (2-3 g/L), Zn- and Cu- (total metal 0-500 mg/L) containing wastewater was studied in a four-stage anaerobic baffled reactor (ABR) at 35 °C for 250 days. Ethanol was supplemented (COD/SO4(2-) = 0.67) as carbon and electron source for sulfate reducing bacteria. Sulfate reduct...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-010-0423-9

    authors: Sahinkaya E,Yucesoy Z

    更新日期:2010-10-01 00:00:00

  • Biomimetic oxidase sensor based on functionalized surface of carbon nanotubes and iron prophyrins for catechol detection.

    abstract::A novel and highly stable biomimetic oxidase sensor system was designed for catehol detection. FePP used as biomimetic horseradish peroxidase (HRP) was immobilized onto modified multi-walled carbon nanotubes (MWCNTs). Functional groups such as -OH, -NH2 and -COOH were introduced onto the surface of MWCNTs to provide b...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-018-2032-y

    authors: Bin Z,Yanhong C,Jiaojiao X

    更新日期:2019-02-01 00:00:00

  • UV photolysis for relieved inhibition of sulfadiazine (SD) to biomass growth.

    abstract::UV photolysis was used to relieve inhibition of biomass growth by sulfadiazine (SD), a broad-spectrum anti-microbial. To investigate the effects of SD on biomass growth, three substrates-glucose alone (G), glucose plus sulfadiazine (G+SD), and glucose plus photolyzed SD (G+PSD)-were used to culture the bacteria acclim...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-014-1335-x

    authors: Pan S,Yan N,Zhang Y,Rittmann BE

    更新日期:2015-05-01 00:00:00

  • Plasmid DNA primary recovery from E. coli lysates by depth bed microfiltration.

    abstract::Recently, several studies have been published on the application of plasmid DNA (pDNA) in gene therapy and vaccine production. The bioprocess to obtain pDNA involves the steps of fermentation, primary recovery, secondary recovery and final purification. The pDNA primary recovery, which is the key step to the rest of t...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-015-1351-5

    authors: Padilla-Zamudio A,Guerrero-Germán P,Tejeda-Mansir A

    更新日期:2015-06-01 00:00:00

  • Surfactant removal from wastewater using photo-cathode microbial fuel cell and laterite-based hybrid treatment system.

    abstract::Sodium dodecyl sulfate (SDS) is a widely used anionic surfactant, which finds its way to the receiving water body due to the incapability of conventional wastewater treatment systems to completely remove it. A hybrid treatment system consisting of upflow microbial fuel cell (MFC) with titanium dioxide (TiO2) as a phot...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-020-02396-4

    authors: Sathe SM,Bhowmick GD,Dubey BK,Ghangrekar MM

    更新日期:2020-11-01 00:00:00

  • A metabolic network-based approach for developing feeding strategies for CHO cells to increase monoclonal antibody production.

    abstract::Chinese hamster ovary (CHO) cells are the main workhorse in the biopharmaceutical industry for the production of recombinant proteins, such as monoclonal antibodies. To date, a variety of metabolic engineering approaches have been used to improve the productivity of CHO cells. While genetic manipulations are potential...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-020-02332-6

    authors: Fouladiha H,Marashi SA,Torkashvand F,Mahboudi F,Lewis NE,Vaziri B

    更新日期:2020-08-01 00:00:00

  • Mathematical model of organic substrate degradation in solid waste windrow composting.

    abstract::Organic solid waste composting is a complex process that involves many coupled physical, chemical and biological mechanisms. To understand this complexity and to ease in planning, design and management of the composting plant, mathematical model for simulation is usually applied. The aim of this paper is to develop a ...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-015-1492-6

    authors: Seng B,Kristanti RA,Hadibarata T,Hirayama K,Katayama-Hirayama K,Kaneko H

    更新日期:2016-01-01 00:00:00

  • Continuous degradation of maltose: improvement in stability and catalytic properties of maltase (α-glucosidase) through immobilization using agar-agar gel as a support.

    abstract::Maltose degrading enzyme was immobilized within agar-agar support via entrapment method due to its industrial utilization. The maximum immobilization efficiency (82.77%) was achieved using 4.0% agar-agar keeping the diameter of bead up to 3.0 mm. The matrix entrapment showed maximum catalytic activity at pH 7.0 and te...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-014-1302-6

    authors: Nawaz MA,Karim A,Aman A,Marchetti R,Qader SA,Molinaro A

    更新日期:2015-04-01 00:00:00

  • Mercury sensing and toxicity studies of novel latex fabricated silver nanoparticles.

    abstract::Safe and eco-friendly alternatives to currently used hazardous chemico-physical methods of silver nanoparticles (AgNPs) synthesis are need of time. Rapid, low cost, selective detection of toxic metals in environmental sample is important to take safety action. Toxicity assessment of engineered AgNPs is essential to av...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-014-1200-y

    authors: Borase HP,Patil CD,Salunkhe RB,Suryawanshi RK,Salunke BK,Patil SV

    更新日期:2014-11-01 00:00:00

  • Effect of the N/P ratio on biomass productivity and nutrient removal from municipal wastewater.

    abstract::The aim of this study is to investigate the effect of the N/P ratio on biomass growth with the simultaneous removal of nutrients from municipal wastewaters. An optical panel photobioreactor is employed for this investigation because it provides a uniform light distribution within the reactor, which enhances the effici...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-014-1317-z

    authors: Choi HJ,Lee SM

    更新日期:2015-04-01 00:00:00

  • Improvement of ethanol production by extractive fed-batch fermentation in a drop column bioreactor.

    abstract::The use of fed-batch extractive fermentation can overcome inhibitory effects caused by the substrate and ethanol to the yeast cells, since it allows regulate the substrate concentration and remove the product as it is produced. The present study describes the modelling and experimental validation of ethanol production...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-020-02414-5

    authors: Lemos DA,Sonego JLS,Cruz AJG,Badino AC

    更新日期:2020-12-01 00:00:00

  • Highly thermo-halo-alkali-stable β-1,4-endoxylanase from a novel polyextremophilic strain of Bacillus halodurans.

    abstract::A novel bacterial isolate, capable of producing extracellular highly thermostable, halo-alkali-stable and cellulase-free xylanase, was isolated from soil and identified as Bacillus halodurans TSPV1 by polyphasic approach. The Plackett-Burman design identified wheat bran, lactose, tryptone and NaCl as the factors that ...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-012-0811-4

    authors: Kumar V,Syal P,Satyanarayana T

    更新日期:2013-05-01 00:00:00

  • Characterization of Pb2+ biosorption from aqueous solution by Rhodotorula glutinis.

    abstract::The yeast Rhodotorula glutinis was examined for its ability to remove Pb(2+) from aqueous solution. Within 10 min of contact, Pb(2+) sorption reached nearly 80% of the total Pb(2+) sorption. The optimum initial pH value for removal of Pb(2+ )was 4.5-5.0. The percentage sorption increased steeply with the biomass conce...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-002-0315-8

    authors: Cho DH,Kim EY

    更新日期:2003-03-01 00:00:00

  • Influence of oxygen transfer on Pseudomonas putida effects on growth rate and biodesulfurization capacity.

    abstract::The growth rate and desulfurization capacity accumulated by the cells during the growth of Pseudomonas putida KTH2 under different oxygen transfer conditions in a stirred and sparged tank bioreactor have been studied. Hydrodynamic conditions were changed using different agitation conditions. During the culture, severa...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-016-1536-6

    authors: Escobar S,Rodriguez A,Gomez E,Alcon A,Santos VE,Garcia-Ochoa F

    更新日期:2016-04-01 00:00:00

  • Anaerobic removal of 1-methoxy-2-propanol under ambient temperature in an EGSB reactor.

    abstract::Two laboratory-scale expanded granular sludge bed (EGSB) reactors were operated at 18 and 25 °C, respectively, for the treatment of synthetic wastewater composed of ethanol and 1-methoxy-2-propanol (M2P) in a mass ratio of 4:1. Reactors were operated first with continuous wastewater supply and after with discontinuous...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-015-1453-0

    authors: Lafita C,Penya-roja JM,Gabaldón C

    更新日期:2015-11-01 00:00:00

  • High-throughput optimization of the chemically defined synthetic medium for the production of erythromycin A.

    abstract::Erythromycin A is an important antibiotic. A chemically defined synthetic medium for erythromycin production was systematically optimized in this study. A high-throughput method was employed to reduce the number of components and optimize the concentration of each component. After two round single composition deletion...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-018-1980-6

    authors: Hong M,Liao J,Chu J

    更新日期:2018-10-01 00:00:00

  • Efficient lipase-catalyzed Knoevenagel condensation: utilization of biocatalytic promiscuity for synthesis of benzylidene-indolin-2-ones.

    abstract::Based on the screening of biocatalysts and reaction conditions including solvent, water content, temperature, enzyme loading, and reaction time, lipase from porcine pancreas (PPL) showed the prominent promiscuity for the Knoevenagel condensation between 1,3-dihydroindol-2-one heterocycle and aromatic aldehydes. Under ...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-015-1496-2

    authors: Ding Y,Xiang X,Gu M,Xu H,Huang H,Hu Y

    更新日期:2016-01-01 00:00:00

  • A general inhibition kinetics model for ethanol production using a novel carbon source: sodium gluconate.

    abstract::In this study, sodium gluconate was applied as a novel carbon source for the fuel ethanol production using an engineered Escherichia coli strain KO11 in batch fermentations. Ethanol and acetic acid were produced as two major products as well as small amount of lactic acid during the fermentation. Compared to the conve...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-013-0938-y

    authors: Wu W,Fan Z

    更新日期:2013-11-01 00:00:00

  • High-level soluble and functional expression of Trigonopsis variabilis D-amino acid oxidase in Escherichia coli.

    abstract::D-Amino acid oxidase is an important biocatalyst used in a variety of fields, and its economically justified level recombinant expression in Escherichia coli has not been established. To accomplish this, after a single Phe54Tyr substitution, fusion proteins of D-amino acid oxidase from Trigonopsis variabilis (TvDAO) w...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-013-1123-z

    authors: Deng S,Su E,Ma X,Yang S,Wei D

    更新日期:2014-08-01 00:00:00

  • Kinetics of carbendazim degradation in a horizontal tubular biofilm reactor.

    abstract::The fungicide carbendazim is an ecotoxic agent affecting aquatic biota. Due to its suspected hormone-disrupting effects, it is considered a "priority hazard substance" by the Water Framework Directive of the European Commission, and its degradation is of major concern. In this work, a horizontal tubular biofilm reacto...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-016-1717-3

    authors: Alvarado-Gutiérrez ML,Ruiz-Ordaz N,Galíndez-Mayer J,Santoyo-Tepole F,Curiel-Quesada E,García-Mena J,Ahuatzi-Chacón D

    更新日期:2017-04-01 00:00:00

  • Acetic acid-catalyzed hydrothermal pretreatment of corn stover for the production of bioethanol at high-solids content.

    abstract::Corn stover (CS) was hydrothermally pretreated using CH3COOH (0.3 %, v/v), and subsequently its ability to be utilized for conversion to ethanol at high-solids content was investigated. Pretreatment conditions were optimized employing a response surface methodology (RSM) with temperature and duration as independent va...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-016-1618-5

    authors: Katsimpouras C,Christakopoulos P,Topakas E

    更新日期:2016-09-01 00:00:00

  • Performance of on-site pilot static granular bed reactor (SGBR) for treating dairy processing wastewater and chemical oxygen demand balance modeling under different operational conditions.

    abstract::The performance and operational stability of a pilot-scale static granular bed reactor (SGBR) for the treatment of dairy processing wastewater were investigated under a wide range of organic and hydraulic loading rates and temperature conditions. The SGBR achieved average chemical oxygen demand (COD), biological oxyge...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-014-1275-5

    authors: Oh JH,Park J,Ellis TG

    更新日期:2015-02-01 00:00:00

  • Applying dimorphic yeasts as model organisms to study mycelial growth: part 1. Experimental investigation of the spatio-temporal development of filamentous yeast colonies.

    abstract::Colony development of the dimorphic yeasts Yarrowia lipolytica and Candida boidinii on solid agar substrates under glucose limitation served as a model system for mycelial development of higher filamentous fungi. Strong differences were observed in the behaviour of both yeasts: C. boidinii colonies reached a final col...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-010-0442-6

    authors: Walther T,Reinsch H,Weber P,Ostermann K,Deutsch A,Bley T

    更新日期:2011-01-01 00:00:00

  • High-level extracellular production of D-Psicose-3-epimerase with recombinant Escherichia coli by a two-stage glycerol feeding approach.

    abstract::The aim of this study is to achieve high-level extracellular production of D-Psicose-3-epimerase (DPE) with recombinant Escherichia coli. High-level production of DPE is one of the key factors in D-Psicose production. In the present study, the gene AAL45544.1 from Agrobacterium tumefaciens str. C58 was modified by art...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-013-0952-0

    authors: Gu L,Zhang J,Liu B,Wu C,Du G,Chen J

    更新日期:2013-11-01 00:00:00

  • Bioprocessing of wheat and paddy straw for their nutritional up-gradation.

    abstract::Solid-state bioprocessing of agricultural residues seems to be an emerging and effective method for the production of high quality animal feed. Seven strains of white-rot fungi were selected to degrade wheat and paddy straw (PS) under solid-state conditions. Degradation of different components, i.e., hemicellulose, ce...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-013-1116-y

    authors: Sharma RK,Arora DS

    更新日期:2014-07-01 00:00:00

  • Kinetic models of ribonucleic acid fermentation and continuous culture by Candida tropicalis no.121.

    abstract::During ribonucleic acid fermentation, the fermentative processes were researched at pH controlled at 4.0 and under natural conditions. Unstructured models in a 50-L airlift fermentor were established for batch RNA production at pH 4.0 using the Verhulst equation for microbial growth, the Luedeking-Piret equation for p...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-011-0580-5

    authors: Li B,Chen X,Ren H,Li L,Xiong J,Bai J,Chen Y,Wu J,Ying H

    更新日期:2012-03-01 00:00:00