A general inhibition kinetics model for ethanol production using a novel carbon source: sodium gluconate.

Abstract:

:In this study, sodium gluconate was applied as a novel carbon source for the fuel ethanol production using an engineered Escherichia coli strain KO11 in batch fermentations. Ethanol and acetic acid were produced as two major products as well as small amount of lactic acid during the fermentation. Compared to the conventional carbon source glucose, the bioconversion of sodium gluconate possessed two distinct advantages: faster utilization rate of sodium gluconate (1.66 g/L per h) compared to glucose (0.996 g/L per h) and no requirement for pH control during fermentation. A general inhibition model including both substrate and products inhibitory effects was proposed, which adequately simulated batch fermentation kinetics at various concentrations of sodium gluconate. All of the products showed inhibitory effects on cell growth. The order of the inhibitory strength of all products and substrate was for the first time clarified in this study. Acetic acid was the most inhibitory product mitigating the cell growth, followed by ethanol and lactic acid. Sodium gluconate stimulated cell growth when its concentration was below 16 g/L, while it inhibited the cell growth when the concentration was above this concentration. It completely inhibited the cell growth when the concentration was 325 g/L. The high value of both the coefficient of determination (R (2)) and the adjusted R (2) verified the good fit of the model. This paper provides key insights into further engineering these strains to improve ethanol production.

journal_name

Bioprocess Biosyst Eng

authors

Wu W,Fan Z

doi

10.1007/s00449-013-0938-y

subject

Has Abstract

pub_date

2013-11-01 00:00:00

pages

1631-40

issue

11

eissn

1615-7591

issn

1615-7605

journal_volume

36

pub_type

杂志文章
  • High-level extracellular production of D-Psicose-3-epimerase with recombinant Escherichia coli by a two-stage glycerol feeding approach.

    abstract::The aim of this study is to achieve high-level extracellular production of D-Psicose-3-epimerase (DPE) with recombinant Escherichia coli. High-level production of DPE is one of the key factors in D-Psicose production. In the present study, the gene AAL45544.1 from Agrobacterium tumefaciens str. C58 was modified by art...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-013-0952-0

    authors: Gu L,Zhang J,Liu B,Wu C,Du G,Chen J

    更新日期:2013-11-01 00:00:00

  • Status of the application of exogenous enzyme technology for the development of natural plant resources.

    abstract::Exogenous enzymes are extraneous enzymes that are not intrinsic to the subject. The exogenous enzyme industry has been rapidly developing recently. Successful application of recombinant DNA amplification, high-efficiency expression, and immobilization technology to genetically engineered bacteria provides a rich sourc...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章,评审

    doi:10.1007/s00449-020-02463-w

    authors: Yuan B,Zhou S,Liu C,Zhang S,Li J,Liu A

    更新日期:2020-11-04 00:00:00

  • Immobilization of horseradish peroxidase onto kaolin.

    abstract::Kaolin showed as a very perspective carrier for the enzyme immobilization and it was used for the adsorption of horseradish peroxidase (HRP). The effects of the enzyme concentration and pH on the immobilization efficiency were studied in the reaction with pyrogallol and anthraquinone dye C.I. Acid Violet 109 (AV 109)....

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-015-1529-x

    authors: Šekuljica NŽ,Prlainović NŽ,Jovanović JR,Stefanović AB,Djokić VR,Mijin DŽ,Knežević-Jugović ZD

    更新日期:2016-03-01 00:00:00

  • Broad-spectrum electrochemical immunosensor based on one-step electrodeposition of AuNP-Abs and Prussian blue nanocomposite for organophosphorus pesticide detection.

    abstract::Broad-spectrum antibodies can effectively recognize substances with similar structures and have broad application prospects in field rapid detection. In this study, broad-spectrum antibodies (Abs) against organophosphorus pesticides (OPs) were used as sensitive recognition elements, which could effectively recognize m...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-020-02472-9

    authors: Dong H,Zhao Q,Li J,Xiang Y,Liu H,Guo Y,Yang Q,Sun X

    更新日期:2020-11-08 00:00:00

  • Remote engineering for a cheese whey biorefinery: an Internet-based application for process design, economic analysis, monitoring, and control of multiple plant sites.

    abstract::The proteolysis of cheese whey with the aid of immobilized enzymes is an attractive alternative for this by-product of the dairy industry. Among some possible applications for whey protein hydrolysates, one may cite their use as protein source for individuals with reduced capacity of digestion, or with genetic metabol...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-008-0222-8

    authors: Pinto GA,Giordano RL,Giordano RC

    更新日期:2009-01-01 00:00:00

  • Production of a recombinant polyester-cleaving hydrolase from Thermobifida fusca in Escherichia coli.

    abstract::The hydrolase (Thermobifida fusca hydrolase; TfH) from T. fusca was produced in Escherichia coli as fusion protein using the OmpA leader sequence and a His(6) tag. Productivity could be raised more than 100-fold. Both batch and fed-batch cultivations yield comparable cell specific productivities whereas volumetric pro...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-006-0069-9

    authors: Dresler K,van den Heuvel J,Müller RJ,Deckwer WD

    更新日期:2006-08-01 00:00:00

  • Asymmetric synthesis of tert-butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate using a self-sufficient biocatalyst based on carbonyl reductase and cofactor co-immobilization.

    abstract::tert-Butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate [(3R,5S)-CDHH] is the key chiral intermediate to synthesize the side chain of the lipid-lowering drug rosuvastatin. Carbonyl reductases showed excellent activity for the biosynthesis of (3R,5S)-CDHH. The requirement of cofactor NADH/NADPH leads to high cost for the in...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-019-02201-x

    authors: Zhang XJ,Wang WZ,Zhou R,Liu ZQ,Zheng YG

    更新日期:2020-01-01 00:00:00

  • Acetic acid-catalyzed hydrothermal pretreatment of corn stover for the production of bioethanol at high-solids content.

    abstract::Corn stover (CS) was hydrothermally pretreated using CH3COOH (0.3 %, v/v), and subsequently its ability to be utilized for conversion to ethanol at high-solids content was investigated. Pretreatment conditions were optimized employing a response surface methodology (RSM) with temperature and duration as independent va...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-016-1618-5

    authors: Katsimpouras C,Christakopoulos P,Topakas E

    更新日期:2016-09-01 00:00:00

  • Sequential pretreatment strategies under mild conditions for efficient enzymatic hydrolysis of wheat straw.

    abstract::This work studies the sequential execution of dilute sulfuric acid (DAP) (0.1-0.75 %, v/v) and dilute sodium hydroxide (AKP) (0.25-3 %, w/v) [i.e., DAP followed by AKP (DAP+AKP) and vice versa (AKP+DAP)] at low temperatures (<121 °C) and short reaction times (5-60 min) for maximizing sugar recovery in the enzymatic hy...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-015-1355-1

    authors: Sanchez A,Gil JC,Rojas-Rejón OA,de Alba AP,Medina A,Flores R,Puente R

    更新日期:2015-06-01 00:00:00

  • Simple, effective protein extraction method and proteomics analysis from polyunsaturated fatty acids-producing micro-organisms.

    abstract::Polyunsaturated fatty acids (PUFAs) are valuable ingredients in the food and pharmaceutical products due to their beneficial influence on human health. Most studies paid attention on the production of PUFAs from oleaginous micro-organisms but seldom on the comparative proteomics of cells. In the study, three methods (...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-015-1467-7

    authors: Ling X,Guo J,Zheng C,Ye C,Lu Y,Pan X,Chen Z,Ng IS

    更新日期:2015-12-01 00:00:00

  • Chemical modification with functionalized ionic liquids: a novel method to improve the enzymatic properties of Candida rugosa lipase.

    abstract::Chemical modification of lysine residues in Candida rugosa lipase (CRL) was carried out using five different functional ionic liquids, and about 15.4-25.0 % of the primary amino groups of lysine were modified. Enzymatic properties of the native and modified CRLs were investigated in olive oil hydrolysis reaction. Impr...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-014-1134-4

    authors: Hu Y,Yang J,Jia R,Ding Y,Li S,Huang H

    更新日期:2014-08-01 00:00:00

  • A metabolic network-based approach for developing feeding strategies for CHO cells to increase monoclonal antibody production.

    abstract::Chinese hamster ovary (CHO) cells are the main workhorse in the biopharmaceutical industry for the production of recombinant proteins, such as monoclonal antibodies. To date, a variety of metabolic engineering approaches have been used to improve the productivity of CHO cells. While genetic manipulations are potential...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-020-02332-6

    authors: Fouladiha H,Marashi SA,Torkashvand F,Mahboudi F,Lewis NE,Vaziri B

    更新日期:2020-08-01 00:00:00

  • Mechanisms of the inhibition of enzymatic hydrolysis of waste pulp fibers by calcium carbonate and the influence of nonionic surfactant for mitigation.

    abstract::Recycled paper mills produce large quantities of fibrous rejects and fines which are usually sent to landfills as solid waste. These cellulosic materials can be enzymatically hydrolyzed into sugars for the production of biofuels and biomaterials. Paper mill wastes also contain large amounts of calcium carbonate which ...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-017-1745-7

    authors: Min BC,Ramarao BV

    更新日期:2017-06-01 00:00:00

  • Impact of operating conditions on performance of a novel gas double-dynamic solid-state fermentation bioreactor (GDSFB).

    abstract::A self-designed novel solid-state fermentation (SSF) bioreactor named "gas double-dynamic solid-state fermentation bioreactor (GDSFB)" showed great success in processes for the production of several valuable products. For the present study, a simple GDSFB (2 L in volume) was designed to investigate the impact of exhau...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-013-0950-2

    authors: Chen H,Li Y,Xu F

    更新日期:2013-11-01 00:00:00

  • Characterization of a commercial cellulase for hydrolysis of agroindustrial substrates.

    abstract::This work is focused on the characterization of a commercial cellulase in terms of optimum pH and temperature, stability to pH and temperature and affinity of this enzyme to several substrates, determining the Michaelis-Menten parameters. Maximum activity of cellulase was obtained for the temperature range from 40 to ...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-012-0710-8

    authors: Balsan G,Astolfi V,Benazzi T,Meireles MA,Maugeri F,Di Luccio M,Dal Prá V,Mossi AJ,Treichel H,Mazutti MA

    更新日期:2012-09-01 00:00:00

  • Effect of organic cosolvent on kinetic resolution of tert-leucine by penicillin G acylase from Kluyvera citrophila.

    abstract::Penicillin G acylase (PGA) from Kluyvera citrophila immobilized on Amberzyml was used for enantioselective hydrolysis of N-phenylacetylated-DL-tert-leucine (N-Phac-DL-Tle) to produce L-tert-leucine (L-Tle). The effects of various organic cosolvents on hydrolysis of N-Phac-DL-Tle have been investigated in aqueous-cosol...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-005-0027-y

    authors: Liu SL,Wei DZ,Song QX,Zhang YW,Wang XD

    更新日期:2006-04-01 00:00:00

  • Isolation and characterization of a serine protease-producing marine bacterium Marinomonas arctica PT-1.

    abstract::A serine protease-producing marine bacterial strain named as PT-1 was isolated and identified as a family of Marinomonas arctica, based on molecular characterization of 16S rRNA gene sequence, phylogenetic tree, and fatty acid composition analyses. Optimized culture conditions for growth of the bacterium PT-1 and prod...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-015-1514-4

    authors: Yoo AY,Park JK

    更新日期:2016-02-01 00:00:00

  • Kinetic and thermodynamic studies on the enzymatic synthesis of wax ester catalyzed by lipase immobilized on glutaraldehyde-activated rice husk particles.

    abstract::Commercial lipase from Thermomyces lanuginosus has been immobilized on glutaraldehyde-activated rice husk particles via covalent attachment. It was reached maximum immobilized protein concentration of 27.5 ± 1.8 mg g-1 of dry support using the initial protein loading of 40 mg g-1 of support. The immobilized biocatalys...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-018-1929-9

    authors: Lima LCD,Peres DGC,Mendes AA

    更新日期:2018-07-01 00:00:00

  • Accelerated start-up of moving bed biofilm reactor by using a novel suspended carrier with porous surface.

    abstract::A novel suspended carrier with porous surface was firstly prepared by coating a sponge on the inside and outside of a hard polyethylene ring. Herein the effects of the sponge thickness (0, 2, 4, 6 mm) and pore size (17, 45, 85 pores per inch, ppi) on the performance of the start-up stage in moving bed biofilm reactor ...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-014-1266-6

    authors: Chen X,Kong L,Wang X,Tian S,Xiong Y

    更新日期:2015-02-01 00:00:00

  • Modulation of lipase B from Candida antarctica properties via covalent immobilization on eco-friendly support for enzymatic kinetic resolution of rac-indanyl acetate.

    abstract::In this study, the modulation of enzymatic biocatalysts were developed by the use of lipase B from Candida antarctica covalently immobilized on an eco-friendly support, cashew apple bagasse, activated with 10% glycidol-ethylenediamine-glutaraldehyde (GEG) under different immobilization strategies (5 mM or 100 mM ionic...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-020-02411-8

    authors: de Souza TC,de Sousa Fonseca T,de Sousa Silva J,Lima PJM,Neto CACG,Monteiro RRC,Rocha MVP,de Mattos MC,Dos Santos JCS,Gonçalves LRB

    更新日期:2020-12-01 00:00:00

  • Solvent toxicity in organic-aqueous systems analysed by multivariate analysis.

    abstract::The effect of several solvents present in a biphasic reaction system on cells of Rhodococcus erythropolis DCL14, Xanthobacter Py2, Arthrobacter simplex and Mycobacterium sp. NRRL B-3805 was evaluated. These four strains have been widely exploited, from bioremediation to the production of fine chemicals, in two-phase r...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-004-0381-1

    authors: de Carvalho CC,da Fonseca MM

    更新日期:2004-12-01 00:00:00

  • Strain and plastic composite support (PCS) selection for vitamin K (Menaquinone-7) production in biofilm reactors.

    abstract::Menaquinone-7 (MK-7), a subtype of vitamin K, has received a significant attention due to its effect on improving bone and cardiovascular health. Current fermentation strategies, which involve static fermentation without aeration or agitation, are associated with low productivity and scale-up issues and hardly justify...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-017-1807-x

    authors: Mahdinia E,Demirci A,Berenjian A

    更新日期:2017-10-01 00:00:00

  • Influence of photobioreactor configuration on microalgal biomass production.

    abstract::Biodiesel production from microalgae depends on the biomass concentration and lipid content in microalgal cells. Photobioreactors (PBRs) facilitates cultivation of microalgae and renders better process control than open systems. However, reactor configuration and consequential hydrodynamics considerably influence biom...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-020-02342-4

    authors: Banerjee S,Dasgupta S,Das D,Atta A

    更新日期:2020-08-01 00:00:00

  • Esterification of polyglycerol with polycondensed ricinoleic acid catalysed by immobilised Rhizopus oryzae lipase.

    abstract::The enzymatic method for synthesising polyglycerol polyricinoleate (PGPR), a food additive named E-476, was successfully carried out in the presence of immobilised Rhizopus oryzae lipase in a solvent-free medium. The great advantage of using the commercial preparation of R. oryzae lipase is that it is ten times cheape...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-012-0874-2

    authors: Ortega S,Máximo MF,Montiel MC,Murcia MD,Arnold G,Bastida J

    更新日期:2013-09-01 00:00:00

  • Quantitative metabolic flux analysis revealed uneconomical utilization of ATP and NADPH in Acremonium chrysogenum fed with soybean oil.

    abstract::A metabolic network was constructed for the Acremonium chrysogenum cultivation fed with soybean oil. Metabolic flux analysis indicated that the shift from exponential growth to rapid cephalosporin C (CPC) formation was accompanied by 1.63- and 5-fold carbon flux enlargement in TCA cycle and glyoxylate by-pass, respect...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-010-0439-1

    authors: Li J,Yang Y,Chu J,Huang M,Li L,Zhang X,Wang Y,Zhuang Y,Zhang S

    更新日期:2010-11-01 00:00:00

  • Construction of a high efficiency copper adsorption bacterial system via peptide display and its application on copper dye polluted wastewater.

    abstract::For the construction of an efficient copper waste treatment system, a cell surface display strategy was employed. The copper adsorption ability of recombinant bacterial strains displaying three different copper binding peptides were evaluated in LB Luria-Bertani medium (LB), artificial wastewater, and copper phthalocy...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-015-1447-y

    authors: Maruthamuthu MK,Nadarajan SP,Ganesh I,Ravikumar S,Yun H,Yoo IK,Hong SH

    更新日期:2015-11-01 00:00:00

  • Biological nitrogen and phosphorus removal in membrane bioreactors: model development and parameter estimation.

    abstract::Membrane bioreactors (MBR) are being increasingly used for wastewater treatment. Mathematical modeling of MBR systems plays a key role in order to better explain their characteristics. Several MBR models have been presented in the literature focusing on different aspects: biological models, models which include solubl...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-012-0806-1

    authors: Cosenza A,Mannina G,Neumann MB,Viviani G,Vanrolleghem PA

    更新日期:2013-04-01 00:00:00

  • Layer-by-layer construction of graphene-based microbial fuel cell for improved power generation and methyl orange removal.

    abstract::Development of highly efficient anode is critical for enhancing the power output of microbial fuel cells (MFCs). The aim of this work is to investigate whether modification of carbon paper (CP) anode with graphene (GR) via layer-by-layer assembly technique is an effective approach to promote the electricity generation...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-014-1148-y

    authors: Guo W,Cui Y,Song H,Sun J

    更新日期:2014-09-01 00:00:00

  • Biomimetic oxidase sensor based on functionalized surface of carbon nanotubes and iron prophyrins for catechol detection.

    abstract::A novel and highly stable biomimetic oxidase sensor system was designed for catehol detection. FePP used as biomimetic horseradish peroxidase (HRP) was immobilized onto modified multi-walled carbon nanotubes (MWCNTs). Functional groups such as -OH, -NH2 and -COOH were introduced onto the surface of MWCNTs to provide b...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-018-2032-y

    authors: Bin Z,Yanhong C,Jiaojiao X

    更新日期:2019-02-01 00:00:00

  • Newly isolated Bacillus sp. G51 from Patagonian wool produces an enzyme combination suitable for felt-resist treatments of organic wool.

    abstract::Bacteria from Patagonian Merino wool were isolated to assess their wool-keratinolytic activity and potential for felt-resist treatments. Strains from Bacillus, Exiguobacterium, Deinococcus, and Micrococcus produced wool-degrading enzymes. Bacillus sp. G51 showed the highest wool-keratinolytic activity. LC-MS/MS analys...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-017-1748-4

    authors: Iglesias MS,Sequeiros C,García S,Olivera NL

    更新日期:2017-06-01 00:00:00