Abstract：
:Amano lipase AK from P. fluorescens was immobilized on different types of chitosan-containing supports. Chitosan lower molecular weight (2.5%), chitosan lower molecular weight/sodium alginate (2.5%/2.5%) and chitosan lower molecular weight/carrageenan (2.5%/2.5%) allowed the highest values of immobilization yields (IY) of 81, 81 and 83%, respectively. Best activity results were achieved using chitosan average molecular weight (5%) and chitosan lower molecular weight/sodium alginate (2.5%/2.5%) as support, with values of 1.40 and 1.30 UpNPB/ggel and with recovery activities of 45.75 and 35.6%, respectively. These derivatives were evaluated in the kinetic resolution of rac-indanol to obtain a key intermediate in the synthesis of a drug used in the treatment of Parkinson's disease. The most efficient derivatives in the kinetic resolution were lipase immobilized on chitosan average molecular weight (5.0%) and chitosan low molecular weight/sodium alginate, the latter leading to obtaining both (S)-indanol and (R)-indanyl acetate with > 99% ee and 50% conversion.
journal_name
Bioprocess Biosyst Engjournal_title
Bioprocess and biosystems engineeringauthors
de Sousa Fonseca T,de Oliveira UMF,de Oliveira MDCF,de Lemos TLG,da Silva MR,Rios NS,Gonçalves LRB,de Mattos MCdoi
10.1007/s00449-020-02487-2subject
Has Abstractpub_date
2021-01-03 00:00:00eissn
1615-7591issn
1615-7605pii
10.1007/s00449-020-02487-2pub_type
杂志文章abstract：:Commercial lipase from Thermomyces lanuginosus has been immobilized on glutaraldehyde-activated rice husk particles via covalent attachment. It was reached maximum immobilized protein concentration of 27.5 ± 1.8 mg g-1 of dry support using the initial protein loading of 40 mg g-1 of support. The immobilized biocatalys...
journal_title：Bioprocess and biosystems engineering
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journal_title：Bioprocess and biosystems engineering
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journal_title：Bioprocess and biosystems engineering
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journal_title：Bioprocess and biosystems engineering
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doi：10.1007/s00449-007-0142-z
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abstract：:Phosphatidylserine is widely used in food, health, chemical and pharmaceutical industries. The phospholipase D-mediated green synthesis of phosphatidylserine has attracted substantial attention in recent years. In this study, the phospholipase D was heterologously expressed in Bacillus subtilis, Pichia pastoris, and C...
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更新日期：2004-12-01 00:00:00
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更新日期：2004-07-01 00:00:00
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更新日期：2013-11-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章,评审
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更新日期：2017-04-01 00:00:00
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更新日期：2015-02-01 00:00:00
abstract：:The aim of the present study was to investigate the feasibility of several food-processing wastes as support substrate for lipolytic enzymes production by the fungus Rhizopus oryzae under solid-state conditions. Different experiments were conducted to select the variables that allow obtaining high levels of lipolytic ...
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abstract：:Recently, several studies have been published on the application of plasmid DNA (pDNA) in gene therapy and vaccine production. The bioprocess to obtain pDNA involves the steps of fermentation, primary recovery, secondary recovery and final purification. The pDNA primary recovery, which is the key step to the rest of t...
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更新日期：2016-09-01 00:00:00
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更新日期：2012-01-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
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abstract：:Gamma-aminobutyric acid (GABA) production by free and Ca-alginate encapsulated cells of Enterococcus faecium CFR 3003 was investigated. Mass transfer rates characterizing the GABA production process using encapsulated cells were investigated. Experiments were performed to investigate external film and internal pore di...
journal_title：Bioprocess and biosystems engineering
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