Abstract：
:An optimized repeated-fed-batch fermentation process for the synthesis of dihydroxyacetone (DHA) from glycerol utilizing Gluconobacter oxydans is presented. Cleaning, sterilization, and inoculation procedures could be reduced significantly compared to the conventional fed-batch process. A stringent requirement was that the product concentration was kept below a critical threshold level at all times in order to avoid irreversible product inhibition of the cells. On the basis of experimentally validated model calculations, a threshold value of about 60 kg x m(-3) DHA was obtained. The innovative bioreactor system consisted of a stirred tank reactor combined with a packed trickle-bed column. In the packed column, active cells could be retained by in situ immobilization on a hydrophilized Ralu-ring carrier material. Within 17 days, the productivity of the process could be increased by 75% to about 2.8 kg x m(-3) h(-1). However, it was observed that the maximum achievable productivity had not been reached yet.
journal_name
Bioprocess Biosyst Engjournal_title
Bioprocess and biosystems engineeringauthors
Hekmat D,Bauer R,Fricke Jdoi
10.1007/s00449-003-0338-9subject
Has Abstractpub_date
2003-12-01 00:00:00pages
109-16issue
2eissn
1615-7591issn
1615-7605journal_volume
26pub_type
杂志文章abstract：:The present study aimed to determine the degradation and transformation of three-ring PAHs phenanthrene and anthracene by Cryptococcus sp. MR22 and Halomonas sp. BR04 under halophilic conditions. The growth progress of Cryptococcus sp. MR22 and Halomonas sp. BR04 on anthracene and phenanthrene was monitored by colony-...
journal_title：Bioprocess and biosystems engineering
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abstract：:Exogenous enzymes are extraneous enzymes that are not intrinsic to the subject. The exogenous enzyme industry has been rapidly developing recently. Successful application of recombinant DNA amplification, high-efficiency expression, and immobilization technology to genetically engineered bacteria provides a rich sourc...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章,评审
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abstract：:Laccases are versatile oxidases that are capable of decolorizing various synthetic dyes. Recombinant Bacillus amyloliquefaciens laccase was immobilized as magnetic cross-linked enzyme aggregates (M-CLEAs) for application in dye decolorization. Several parameters influencing the activity recovery were evaluated during ...
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doi：10.1007/s00449-020-02481-8
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abstract：:Lactococcus lactis spp. lactis and Lactococcus lactis spp. cremoris are widely used in the manufacture of fermented milk. These strains were compared for production of Dipeptidyl Peptidase IV (DPP IV) enzyme in terms of enzyme activity, specific growth rates and productivity. Lactococcus lactis spp. lactis was produce...
journal_title：Bioprocess and biosystems engineering
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abstract：:Salmonella is a major pathogen that causes acute foodborne outbreaks worldwide. Seafood, particularly shellfish, is a proven source of Salmonella spp. infection because many people prefer to eat it raw or lightly cooked. However, traditional identification methods are too time-consuming and complex to detect contamina...
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doi：10.1007/s00449-018-1895-2
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abstract：:The performance and operational stability of a pilot-scale static granular bed reactor (SGBR) for the treatment of dairy processing wastewater were investigated under a wide range of organic and hydraulic loading rates and temperature conditions. The SGBR achieved average chemical oxygen demand (COD), biological oxyge...
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abstract：:Long-term and excessive use of monocrotophos (MPs) pesticide leads to an accumulation of MPs residues in agricultural products. Electrochemical biosensor technology was developed as a simple and efficient method for detecting MPs. However, commercial acetylcholinesterase (AChE) sensors are not applied in practical MPs...
journal_title：Bioprocess and biosystems engineering
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journal_title：Bioprocess and biosystems engineering
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journal_title：Bioprocess and biosystems engineering
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更新日期：2013-12-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章,评审
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-011-0630-z
更新日期：2012-01-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-013-1081-5
更新日期：2014-06-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
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journal_title：Bioprocess and biosystems engineering
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journal_title：Bioprocess and biosystems engineering
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doi：10.1007/s00449-019-02238-y
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journal_title：Bioprocess and biosystems engineering
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journal_title：Bioprocess and biosystems engineering
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journal_title：Bioprocess and biosystems engineering
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