当前位置： SCI文献检索 > BIOPROCESS AND BIOSYSTEMS ENGINEERING期刊下所有文献 > Recombinase polymerase amplification combined with lateral flow dipstick for equipment-free detection of Salmonella in shellfish.

Recombinase polymerase amplification combined with lateral flow dipstick for equipment-free detection of Salmonella in shellfish.

Abstract：

:Salmonella is a major pathogen that causes acute foodborne outbreaks worldwide. Seafood, particularly shellfish, is a proven source of Salmonella spp. infection because many people prefer to eat it raw or lightly cooked. However, traditional identification methods are too time-consuming and complex to detect contamination of bacteria in the food chain in a timely manner, and few studies have aimed to identify Salmonella in shellfish early in the supply chain. We herein developed a method for rapid detection of Salmonella in shellfish based on the method of recombinase polymerase amplification (RPA) combined with lateral flow dipstick (LFD), which targets the invasion gene A (invA). The RPA-LFD was able to function at 30-45 °C, and at the temperature of 40 °C, it only took 8 min of amplification to reach the test threshold of amplicons. The established method had both a good specificity and a sensitivity of 100 fg DNA per reaction (20 µL). Regarding practical performance, RPA-LFD performed better than real-time PCR. Another advantage of RPA-LFD is that it was capable of being performed without expensive equipments. Thus, RPA-LFD has potential for further development as a detection kit for Salmonella in shellfish and other foods under field conditions.

journal_name

Bioprocess Biosyst Eng

journal_title

Bioprocess and biosystems engineering

authors

Gao W,Huang H,Zhu P,Yan X,Fan J,Jiang J,Xu J

doi

10.1007/s00449-018-1895-2

subject

Has Abstract

pub_date

2018-05-01 00:00:00

pages

603-611

issue

5

eissn

1615-7591

issn

1615-7605

pii

10.1007/s00449-018-1895-2

journal_volume

41

pub_type

杂志文章

相关文献

BIOPROCESS AND BIOSYSTEMS ENGINEERING文献大全
  • Continuous degradation of maltose: improvement in stability and catalytic properties of maltase (α-glucosidase) through immobilization using agar-agar gel as a support.

    abstract：:Maltose degrading enzyme was immobilized within agar-agar support via entrapment method due to its industrial utilization. The maximum immobilization efficiency (82.77%) was achieved using 4.0% agar-agar keeping the diameter of bead up to 3.0 mm. The matrix entrapment showed maximum catalytic activity at pH 7.0 and te...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-014-1302-6

    authors： Nawaz MA,Karim A,Aman A,Marchetti R,Qader SA,Molinaro A

    更新日期：2015-04-01 00:00:00

  • Simultaneous saccharification and fermentation of broken rice: an enzymatic extrusion liquefaction pretreatment for Chinese rice wine production.

    abstract：:Broken rice, pretreated by enzymatic extrusion liquefaction, was used to produce Chinese rice wine by simultaneous saccharification and fermentation (SSF) process in this study. The study compared the novel process and traditional process for Chinese rice wine fermentation utilizing broken rice and head rice, respecti...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-012-0868-0

    authors： Li H,Jiao A,Xu X,Wu C,Wei B,Hu X,Jin Z,Tian Y

    更新日期：2013-08-01 00:00:00

  • Effects of fed-batch and continuous fermentations on human lysozyme production by Kluyveromyces lactis K7 in biofilm reactors.

    abstract：:Lysozyme is a lytic enzyme, which has antimicrobial activity. It has been used for food and pharmaceutical applications. This study was undertaken to evaluate fed-batch and continuous fermentations for the human lysozyme production in biofilm reactor. Results showed that addition of lactose the mid-log phase to make t...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-015-1483-7

    authors： Ercan D,Demirci A

    更新日期：2015-12-01 00:00:00

  • Ca2+ increases the specific coenzyme Q10 content in Agrobacterium tumefaciens.

    abstract：:Of various metal ions (Ca2+, Cr3+, Cu2+, Fe2+, Mg2+, Mn2+, Ni2+ and Zn2+) added to the culture medium of Agrobacterium tumefaciens at 1 mM, only Ca2+ increased Coenzyme Q10 (CoQ(10)) content in cells without the inhibition of cell growth. In a pH-stat fed-batch culture, supplementation with 40 mM of CaCO3 increased th...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-009-0318-9

    authors： Ha SJ,Kim SY,Seo JH,Jeya M,Zhang YW,Ramu T,Kim IW,Lee JK

    更新日期：2009-08-01 00:00:00

  • Whole cell bioconversion of vitamin D3 to calcitriol using Pseudonocardia sp. KCTC 1029BP.

    abstract：:Calcitriol is an important drug used for treating osteoporosis, which can be produced from vitamin D3. The current method of producing calcitriol from vitamin D3 during cultivation of microbial cells results in low yields of calcitriol and high purification costs. Therefore, in this study, the steps of cell cultivatio...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-015-1368-9

    authors： Kang DJ,Im JH,Kang JH,Kim KH

    更新日期：2015-07-01 00:00:00

  • Preparation and synthetic dye decolorization ability of magnetic cross-linked enzyme aggregates of laccase from Bacillus amyloliquefaciens.

    abstract：:Laccases are versatile oxidases that are capable of decolorizing various synthetic dyes. Recombinant Bacillus amyloliquefaciens laccase was immobilized as magnetic cross-linked enzyme aggregates (M-CLEAs) for application in dye decolorization. Several parameters influencing the activity recovery were evaluated during ...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-020-02481-8

    authors： Wang H,Han S,Wang J,Yu S,Li X,Lu L

    更新日期：2020-11-27 00:00:00

  • Different strategies for multi-enzyme cascade reaction for chiral vic-1,2-diol production.

    abstract：:The stereoselective three-enzyme cascade for the one-pot synthesis of (1S,2S)-1-phenylpropane-1,2-diol ((1S,2S)-1-PPD) from inexpensive starting substrates, benzaldehyde and acetaldehyde, was explored. By coupling stereoselective carboligation catalyzed by benzoylformate decarboxylase (BFD), L-selective reduction of a...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-018-1912-5

    authors： Presečki AV,Pintarić L,Švarc A,Vasić-Rački Đ

    更新日期：2018-06-01 00:00:00

  • Two-stage pH control combined with oxygen-enriched air strategies for the highly efficient production of EPA by Mortierella alpina CCFM698 with fed-batch fermentation.

    abstract：:Dissolved oxygen and pH are critical factors influencing cell growth and metabolism. In our previous work, we constructed the recombinant strain Mortierella alpina CCFM698, which has the ability to produce EPA at room temperature. However, our experiments showed that the dissolved oxygen produced by the aeration and a...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-020-02367-9

    authors： Huang M,Chen H,Tang X,Lu H,Zhao J,Zhang H,Chen Y,Chen W

    更新日期：2020-09-01 00:00:00

  • Enhancement of nutrient removal performance of activated sludge with a novel hybrid biofilm process.

    abstract：:The aim of this study was to investigate the efficacy of a hybrid biofilm pilot-scale treatment plant, designed with a novel configuration by the integration of a fixed-film system, to improve nitrogen removal. The pilot-scale system was established at a wastewater treatment plant in Istanbul and operated based on str...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-018-2042-9

    authors： Güneş G,Hallaç E,Özgan M,Ertürk A,Okutman Taş D,Çokgor E,Güven D,Takacs I,Erdinçler A,Insel G

    更新日期：2019-03-01 00:00:00

  • Analysis of kinetic uptake phenomena of monosaccharide and disaccharide by suspension TBY-2 cells using an FT-IR/ATR method.

    abstract：:The influence of sugars in culture media on the kinetics of the mono- and disaccharide uptake and cell growth behavior was studied by mid-infrared spectroscopy using a Fourier transform infrared spectrometer (FT-IR) equipped with an attenuate total reflection accessory (ATR). We performed the plant cell cultivation wi...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-007-0142-z

    authors： Yamanaka A,Hashimoto A,Matsuo T,Kanou M,Suehara K,Kameoka T

    更新日期：2007-11-01 00:00:00

  • Oxidases from mate tea leaves (Ilex paraguariensis): extraction optimization and stability at low and high temperatures.

    abstract：:Mate (Ilex paraguariensis) is an important natural product in the economic and cultural context of Brazil. Peroxidase and polyphenol oxidase have been responsible for quality deterioration and browning in mate. The objective of this work was to investigate a methodology of extraction and enzymatic activity determinati...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-007-0196-y

    authors： Ceni GC,Baldissera EM,Antunes OA,Vladimir Oliveira J,Dariva C,de Oliveira D

    更新日期：2008-10-01 00:00:00

  • A novel serine alkaline protease from Bacillus altitudinis GVC11 and its application as a dehairing agent.

    abstract：:A serine alkaline protease from a newly isolated alkaliphilic Bacillus altitudinis GVC11 was purified and characterized. The enzyme was purified to homogeneity by acetone precipitation, DEAE-cellulose anion exchange chromatography with 7.03-fold increase in specific activity and 15.25% recovery. The molecular weight o...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-010-0483-x

    authors： Vijay Kumar E,Srijana M,Kiran Kumar K,Harikrishna N,Reddy G

    更新日期：2011-05-01 00:00:00

  • Hydrogen sulfide formation as well as ethanol production in different media by cysND- and/or cysIJ-inactivated mutant strains of Zymomonas mobilis ZM4.

    abstract：:Many bacteria reduce inorganic sulfate to sulfide to satisfy their need for sulfur, one of the most important elements for biological life. But little is known about the metabolic pathways involving hydrogen sulfide (H₂S) in mesophilic bacteria. By genomic sequence analysis, a complete set of genes for the assimilator...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-012-0839-5

    authors： Tan T,Liu C,Liu L,Zhang K,Zou S,Hong J,Zhang M

    更新日期：2013-10-01 00:00:00

  • Trametes versicolor laccase production using agricultural wastes: a comparative study in Erlenmeyer flasks, bioreactor and tray.

    abstract：:Laccases are very interesting biocatalysts of recognized importance for several industrial applications. Its production by Trametes versicolor, a white-rot fungus, was induced by a combination of cotton gin wastes (1%), a lignocellulosic waste, and vinasse (15%), an industrial by-product from sugarcane industry. The u...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-019-02245-z

    authors： Pinheiro VE,Michelin M,Vici AC,de Almeida PZ,Teixeira de Moraes Polizeli ML

    更新日期：2020-03-01 00:00:00

  • Highly selective resonance scattering detection of trace thrombin using aptamer-modified AuRe nanoprobe.

    abstract：:The gold-rhenium (AuRe) composite nanoparticle was prepared by NaBH(4) reduction procedure, and was modified by the aptamer to obtain an AuRe nanoprobe (AuRessDNA) for thrombin. In pH 7.0 Tris-HCl buffer solution and in the presence of salt, the nanoprobe specifically combined with thrombin to form AuRe-aptamer-thromb...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-010-0434-6

    authors： Liang A,Li J,Jiang C,Jiang Z

    更新日期：2010-11-01 00:00:00

  • Biosynthesis of high-purity γ-MnS nanoparticle by newly isolated Clostridiaceae sp. and its properties characterization.

    abstract：:MnS is a p-type semiconductor with both antiferromagnetism and wide band gap, endowing it potential applications for short wavelength optoelectronic devices, solar cells and luminescent materials. Despite successful biosynthesis of nano CdS, PbS and ZnS with extremely low solubility product, there have been no reports...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-014-1261-y

    authors： Liu X,Wang J,Yue L,Xin B,Chen S,Dai J,Wang R,Wang Y

    更新日期：2015-02-01 00:00:00

  • Mercury sensing and toxicity studies of novel latex fabricated silver nanoparticles.

    abstract：:Safe and eco-friendly alternatives to currently used hazardous chemico-physical methods of silver nanoparticles (AgNPs) synthesis are need of time. Rapid, low cost, selective detection of toxic metals in environmental sample is important to take safety action. Toxicity assessment of engineered AgNPs is essential to av...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-014-1200-y

    authors： Borase HP,Patil CD,Salunkhe RB,Suryawanshi RK,Salunke BK,Patil SV

    更新日期：2014-11-01 00:00:00

  • A metabolic network-based approach for developing feeding strategies for CHO cells to increase monoclonal antibody production.

    abstract：:Chinese hamster ovary (CHO) cells are the main workhorse in the biopharmaceutical industry for the production of recombinant proteins, such as monoclonal antibodies. To date, a variety of metabolic engineering approaches have been used to improve the productivity of CHO cells. While genetic manipulations are potential...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-020-02332-6

    authors： Fouladiha H,Marashi SA,Torkashvand F,Mahboudi F,Lewis NE,Vaziri B

    更新日期：2020-08-01 00:00:00

  • Development of SCAR marker for simultaneous identification of Miscanthus sacchariflorus, M. sinensis and M. x giganteus.

    abstract：:The sequence-characterized amplified region (SCAR) marker for simultaneous identification of Miscanthus sacchariflorus, Miscanthus sinensis, and Miscanthus x giganteus was developed. In this study, it was attempted for the first time to develop the SCAR marker for detecting the molecular phenotypes among Miscanthus sp...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-011-0592-1

    authors： Kim JK,An GH,Ahn SH,Moon YH,Cha YL,Bark ST,Choi YH,Suh SJ,Seo SG,Kim SH,Koo BC

    更新日期：2012-01-01 00:00:00

  • Changes in respiratory quinone profiles of enhanced biological phosphorus removal activated sludge under different influent phosphorus/carbon ratio conditions.

    abstract：:Changes in the microbial community of an enhanced biological phosphorus removal (EBPR) activated sludge system under different influent phosphorus/carbon (P/C) ratio conditions were investigated through evaluation of population respiratory quinone profiles. A total of 13 types of respiratory quinone homologs consistin...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-006-0065-0

    authors： Ahn J,Lee M,Kwon H

    更新日期：2006-08-01 00:00:00

  • A tracer study for assessing the interactions between hydraulic retention time and transport processes in a wetland system for nutrient removal.

    abstract：:In this study, a new-generation subsurface upflow wetland (SUW) system packed with the unique sorption media was introduced for nutrient removal. To explore the interface between hydraulic and environmental performance, a tracer study was carried out in concert with a transport model to collectively provide hydraulic ...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-011-0578-z

    authors： Chang NB,Xuan Z,Wanielista MP

    更新日期：2012-03-01 00:00:00

  • Metabolic engineering of Corynebacterium glutamicum to produce GDP-L-fucose from glucose and mannose.

    abstract：:Wild-type Corynebacterium glutamicum was metabolically engineered to convert glucose and mannose into guanosine 5'-diphosphate (GDP)-L-fucose, a precursor of fucosyl-oligosaccharides, which are involved in various biological and pathological functions. This was done by introducing the gmd and wcaG genes of Escherichia...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-013-0900-z

    authors： Chin YW,Park JB,Park YC,Kim KH,Seo JH

    更新日期：2013-06-01 00:00:00

  • A simple and efficient method for the extraction and separation of menaquinone homologs from wet biomass of Flavobacterium.

    abstract：:Menaquinone homologs (MK-n), that is, MK-4, MK-5, and MK-6, can be produced by the fermentation of Flavobacterium. In this study, we proposed a simple and efficient method for the extraction of menaquinones from wet cells without the process of drying the biomass. Meanwhile, a rapid and effective solution for the sepa...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-017-1851-6

    authors： Wei H,Zhao G,Liu H,Wang H,Ni W,Wang P,Zheng Z

    更新日期：2018-01-01 00:00:00

  • A homofermentative Bacillus sp. BC-001 and its performance as a potential L-lactate industrial strain.

    abstract：:Bacillus sp. BC-001 was first reported as a potent thermotolerant and homofermentative strain for an industrial-scale L-lactate production. In a flask culture, this isolate fermented both glucose and sucrose to lactate with high yield (0.96 and 0.87 g/g) and productivity (2.8 and 2.6 g/L h), respectively. The higher l...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-017-1833-8

    authors： Thitiprasert S,Kodama K,Tanasupawat S,Prasitchoke P,Rampai T,Prasirtsak B,Tolieng V,Piluk J,Assabumrungrat S,Thongchul N

    更新日期：2017-12-01 00:00:00

  • Inhibition of pathogenic bacterial biofilm by biosurfactant produced by Lysinibacillus fusiformis S9.

    abstract：:A biosurfactant producing microbe isolated from a river bank was identified as Lysinibacillus fusiformis S9. It was identified with help of biochemical tests and 16S rRNA gene phylogenetic analysis. The biosurfactant S9BS produced was purified and characterized as glycolipid. The biosurfactant showed remarkable inhibi...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-013-0976-5

    authors： Pradhan AK,Pradhan N,Sukla LB,Panda PK,Mishra BK

    更新日期：2014-02-01 00:00:00

  • Plasmid DNA primary recovery from E. coli lysates by depth bed microfiltration.

    abstract：:Recently, several studies have been published on the application of plasmid DNA (pDNA) in gene therapy and vaccine production. The bioprocess to obtain pDNA involves the steps of fermentation, primary recovery, secondary recovery and final purification. The pDNA primary recovery, which is the key step to the rest of t...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-015-1351-5

    authors： Padilla-Zamudio A,Guerrero-Germán P,Tejeda-Mansir A

    更新日期：2015-06-01 00:00:00

  • Modulation of lipase B from Candida antarctica properties via covalent immobilization on eco-friendly support for enzymatic kinetic resolution of rac-indanyl acetate.

    abstract：:In this study, the modulation of enzymatic biocatalysts were developed by the use of lipase B from Candida antarctica covalently immobilized on an eco-friendly support, cashew apple bagasse, activated with 10% glycidol-ethylenediamine-glutaraldehyde (GEG) under different immobilization strategies (5 mM or 100 mM ionic...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-020-02411-8

    authors： de Souza TC,de Sousa Fonseca T,de Sousa Silva J,Lima PJM,Neto CACG,Monteiro RRC,Rocha MVP,de Mattos MC,Dos Santos JCS,Gonçalves LRB

    更新日期：2020-12-01 00:00:00

  • Enhanced succinic acid production under acidic conditions by introduction of glutamate decarboxylase system in E. coli AFP111.

    abstract：:Biological synthesis of succinic acid at low pH values was favored since it not only decreased investment cost but also simplified downstream purification process. In this study, the feasibility of using glutamate decarboxylase system to improve succinic acid production of Escherichia coli AFP111, a succinate-producin...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-016-1720-8

    authors： Wu M,Li X,Guo S,Lemma WD,Zhang W,Ma J,Jia H,Wu H,Jiang M,Ouyang P

    更新日期：2017-04-01 00:00:00

  • Toxic effect of environmentally relevant concentration of silver nanoparticles on environmentally beneficial bacterium Pseudomonas putida.

    abstract：:Silver nanoparticles (Ag NPs) are being increasingly used in many consumer products owing to their excellent antimicrobial properties. The continuous use of Ag NPs in consumer products will lead to environmental release. The present study evaluated the toxic effects and the possible underlying mechanism of Ag NPs on P...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-015-1365-z

    authors： Khan SS,Ghouse SS,Chandran P

    更新日期：2015-07-01 00:00:00

  • Optimization of the microbial synthesis of dihydroxyacetone from glycerol with Gluconobacter oxydans.

    abstract：:An optimized repeated-fed-batch fermentation process for the synthesis of dihydroxyacetone (DHA) from glycerol utilizing Gluconobacter oxydans is presented. Cleaning, sterilization, and inoculation procedures could be reduced significantly compared to the conventional fed-batch process. A stringent requirement was tha...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-003-0338-9

    authors： Hekmat D,Bauer R,Fricke J

    更新日期：2003-12-01 00:00:00