Abstract：
:Menaquinone homologs (MK-n), that is, MK-4, MK-5, and MK-6, can be produced by the fermentation of Flavobacterium. In this study, we proposed a simple and efficient method for the extraction of menaquinones from wet cells without the process of drying the biomass. Meanwhile, a rapid and effective solution for the separation of menaquinone homologs was developed using a single organic solvent, which was conducive to the recovery of the solvent. The results showed that the highest yield was obtained with pretreatment using absolute ethanol at a ratio of 6:1 (v/m) for 30 min and then two extractions of 30 min each using methanol at a ratio of 6:1 (v/m). The recovery efficiency of the menaquinones reached to 102.8% compared to the positive control. The menaquinone homologs were effectively separated using methanol as eluent at a flow rate of 0.52 mL/min by a glass reverse-phase C18 silica gel column with a height-to-diameter ratio of 5.5:1. The recovery of menaquinones achieved was 99.6%. In conclusion, the methods for extraction and separation of menaquinone homologs from wet Flavobacterium cells were simple and efficient, which makes them suitable not only on a laboratory scale but also for application on a large scale.
journal_name
Bioprocess Biosyst Engjournal_title
Bioprocess and biosystems engineeringauthors
Wei H,Zhao G,Liu H,Wang H,Ni W,Wang P,Zheng Zdoi
10.1007/s00449-017-1851-6subject
Has Abstractpub_date
2018-01-01 00:00:00pages
107-113issue
1eissn
1615-7591issn
1615-7605pii
10.1007/s00449-017-1851-6journal_volume
41pub_type
杂志文章abstract：:In this study, the silica nanoparticles surface was modified by carboxylic acid with different number of alkyl chain and two different lipases were immobilized on it to increase the glycerolysis activity of the immobilized lipases in organic solvent system. Silica nanoparticles of about 15 nm diameter were grafted wit...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
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pub_type： 杂志文章,评审
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journal_title：Bioprocess and biosystems engineering
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