Optical device for parallel online measurement of dissolved oxygen and pH in shake flask cultures.

Abstract:

:We describe a new device with parallel optical measurement of dissolved oxygen (DO) and pH in up to nine shake flasks applicable in any conventional shaking incubator. Measurement ranges are 0-500% of air saturation for oxygen and 5.5-8.5 for pH. It was used to characterize growth profiles of different L-lysine producing strains of Corynebacterium glutamicum, of Saccharomyces cerevisiae and of Escherichia coli. Cultures in unbaffled flasks were highly reproducible. Oxygen limitation was indicated online which is particularly important when cultivating fast growing cells as E. coli. C. glutamicum strains showed distinct characteristic patterns of DO and pH indicating biological events. During the cultivation of S. cerevisiae on glucose, fructose and galactose, oxygen uptake rate was determined using the predetermined value of k(L)a. pH measurement was used to determine the minimum buffer requirement for a culture of C. glutamicum.

journal_name

Bioprocess Biosyst Eng

authors

Schneider K,Schütz V,John GT,Heinzle E

doi

10.1007/s00449-009-0367-0

subject

Has Abstract

pub_date

2010-06-01 00:00:00

pages

541-7

issue

5

eissn

1615-7591

issn

1615-7605

journal_volume

33

pub_type

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