Biotransformation of lysine into cadaverine using barium alginate-immobilized Escherichia coli overexpressing CadA.

Abstract:

:In this study, Escherichia coli cells overexpressing lysine decarboxylase (CadA) were used for cadaverine production. Barium alginate was selected as a matrix for immobilization of E. coli YH91. Free cells and immobilized cells (IC) were characterized for their physiochemical properties, and the optimum pH and temperature were determined as 6 and 37 °C, respectively. Immobilized cells were three times more thermally stable compared to free cells at the optimum temperature and had a half-life (t 1/2) of 131 h. The free cells lost most of lysine decarboxylase activity after nine cycles, but in contrast immobilized cells retained 56% of their residual activity even after the 18th cycle. The immobilized cells gave a maximum production of cadaverine (75.8 g/L) with 84% conversion.

journal_name

Bioprocess Biosyst Eng

authors

Bhatia SK,Kim YH,Kim HJ,Seo HM,Kim JH,Song HS,Sathiyanarayanan G,Park SH,Park K,Yang YH

doi

10.1007/s00449-015-1465-9

subject

Has Abstract

pub_date

2015-12-01 00:00:00

pages

2315-22

issue

12

eissn

1615-7591

issn

1615-7605

pii

10.1007/s00449-015-1465-9

journal_volume

38

pub_type

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