Abstract：
:The characterization of anodic microbial communities is of great importance in the study of microbial fuel cells (MFCs). These kinds of devices mainly require a high abundance of anode respiring bacteria (ARB) in the anode chamber for optimal performance. This study evaluated the effect of different enrichments of environmental freshwater sediment samples used as inocula on microbial community structures in MFCs. Two enrichment media were compared: ferric citrate (FeC) enrichment, with the purpose of increasing the ARB percentage, and general enrichment (Gen). The microbial community dynamics were evaluated by polymerase chain reaction followed by denaturing gradient gel electrophoresis (PCR-DGGE) and real time polymerase chain reaction (qPCR). The enrichment effect was visible on the microbial community composition both during precultures and in anode MFCs. Both enrichment approaches affected microbial communities. Shannon diversity as well as β-Proteobacteria and γ-Proteobacteria percentages decreased during the enrichment steps, especially for FeC (p < 0.01). Our data suggest that FeC enrichment excessively reduced the diversity of the anode community, rather than promoting the proliferation of ARB, causing a condition that did not produce advantages in terms of system performance.
journal_name
Bioprocess Biosyst Engjournal_title
Bioprocess and biosystems engineeringauthors
Armato C,Ahmed D,Agostino V,Traversi D,Degan R,Tommasi T,Margaria V,Sacco A,Gilli G,Quaglio M,Saracco G,Schilirò Tdoi
10.1007/s00449-019-02074-0subject
Has Abstractpub_date
2019-05-01 00:00:00pages
697-709issue
5eissn
1615-7591issn
1615-7605pii
10.1007/s00449-019-02074-0journal_volume
42pub_type
杂志文章abstract：:Cheese whey fermentation to ethanol using immobilized Kluyveromyces marxianus cells was investigated in batch and continuous operation. In batch fermentation, the yeast cells were immobilized in carboxymethyl cellulose (CMC) polymer and also synthesized graft copolymer of CMC with N-vinyl-2-pyrrolidone, denoted as CMC...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-016-1610-0
更新日期：2016-09-01 00:00:00
abstract：:The sequence-characterized amplified region (SCAR) marker for simultaneous identification of Miscanthus sacchariflorus, Miscanthus sinensis, and Miscanthus x giganteus was developed. In this study, it was attempted for the first time to develop the SCAR marker for detecting the molecular phenotypes among Miscanthus sp...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-011-0592-1
更新日期：2012-01-01 00:00:00
abstract：:Long-term and excessive use of monocrotophos (MPs) pesticide leads to an accumulation of MPs residues in agricultural products. Electrochemical biosensor technology was developed as a simple and efficient method for detecting MPs. However, commercial acetylcholinesterase (AChE) sensors are not applied in practical MPs...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-019-02226-2
更新日期：2020-02-01 00:00:00
abstract：:Soluble microbial products (SMPs) are considered as the main organic components in wastewater treatment plant effluent from biological wastewater treatment systems. To investigate and explore SMP metabolism pathway for further treatment and control, two innovative mechanistically based activated sludge models were dev...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-011-0566-3
更新日期：2011-11-01 00:00:00
abstract：:Commercial lipase from Thermomyces lanuginosus has been immobilized on glutaraldehyde-activated rice husk particles via covalent attachment. It was reached maximum immobilized protein concentration of 27.5 ± 1.8 mg g-1 of dry support using the initial protein loading of 40 mg g-1 of support. The immobilized biocatalys...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-018-1929-9
更新日期：2018-07-01 00:00:00
abstract：:The kinetics of the enzymatic transesterification between a mixture of triglycerides (oils) and methanol for biodiesel production in a bis(2-ethylhexyl) sodium sulfosuccinate (AOT)/isooctane reversed micellar system, using recombinant cutinase from Fusarium solani pisi as a catalyst, was investigated. In order to desc...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-011-0564-5
更新日期：2011-11-01 00:00:00
abstract：:The β-glucanase produced from Bacillus sp. CSB55 not only depicts the potent industrial characteristics but also relates as bio-industrial catalyst supporting the spontaneous formation of the products, high hydrolytic efficiency, and feasibility of the enzymatic reaction. A homogeneous β-glucanase (GluB55) was purifie...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-019-02221-7
更新日期：2020-02-01 00:00:00
abstract：:Rice straw is one of the most abundant renewable energy sources available. Through anaerobic acidogenesis, the substance of rice straw can be converted to volatile fatty acids (VFAs). VFAs itself is of value and is a precursor to biofuels. Hence, it can be converted to mixed alcohols by addition of hydrogen, and biodi...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-015-1357-z
更新日期：2015-06-01 00:00:00
abstract：:Safe and eco-friendly alternatives to currently used hazardous chemico-physical methods of silver nanoparticles (AgNPs) synthesis are need of time. Rapid, low cost, selective detection of toxic metals in environmental sample is important to take safety action. Toxicity assessment of engineered AgNPs is essential to av...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-014-1200-y
更新日期：2014-11-01 00:00:00
abstract：:Gamma-aminobutyric acid (GABA) production by free and Ca-alginate encapsulated cells of Enterococcus faecium CFR 3003 was investigated. Mass transfer rates characterizing the GABA production process using encapsulated cells were investigated. Experiments were performed to investigate external film and internal pore di...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-014-1296-0
更新日期：2015-03-01 00:00:00
abstract：:Development of highly efficient anode is critical for enhancing the power output of microbial fuel cells (MFCs). The aim of this work is to investigate whether modification of carbon paper (CP) anode with graphene (GR) via layer-by-layer assembly technique is an effective approach to promote the electricity generation...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-014-1148-y
更新日期：2014-09-01 00:00:00
abstract：:In this study, the modulation of enzymatic biocatalysts were developed by the use of lipase B from Candida antarctica covalently immobilized on an eco-friendly support, cashew apple bagasse, activated with 10% glycidol-ethylenediamine-glutaraldehyde (GEG) under different immobilization strategies (5 mM or 100 mM ionic...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-020-02411-8
更新日期：2020-12-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-005-0010-7
更新日期：2005-11-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-013-0939-x
更新日期：2013-11-01 00:00:00
abstract：:The biological production of butanol has become an important research field and thanks to genome sequencing and annotation; genome-scale metabolic reconstructions have been developed for several Clostridium species. This work makes use of the iCAC490 model of Clostridium acetobutylicum ATCC 824 to analyze its metaboli...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-015-1513-5
更新日期：2016-02-01 00:00:00
abstract：:We study the adhesion limit of 3T6 fibroblasts, cultured in Dulbecco's modified Eagle's medium with 10% fetal bovine serum at 37 °C and 5% CO2, with a narrow-gap rotational rheometer in the parallel-disk configuration. Reducing the uncertainty in gap width to about 1 µm allows studying the cells at narrow gaps, which ...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-017-1868-x
更新日期：2018-03-01 00:00:00
abstract：:High amounts of outer membrane (OM) components were released in glucose-limited fed-batch (GLFB) cultures at 37 degrees C at specific growth rates approaching 0.05 h(-1). Endotoxin analyses from a 20 degrees C GLFB culture gave similar results. An alternative fermentation technique, the temperature-limited fed-batch (...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-004-0377-x
更新日期：2005-04-01 00:00:00
abstract：:Lipase (LP) was immobilized on electrospun and ethanol-dispersed polystyrene-poly(styrene-co-maleic anhydride) (PS-PSMA) nanofibers (EtOH-NF) in the form of enzyme precipitate coatings (EPCs). LP precipitate coatings (EPCs-LP) were prepared in a three-step process, consisting of covalent attachment, LP precipitation, ...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-011-0534-y
更新日期：2011-09-01 00:00:00
abstract：:Bio-catalytic in vitro multistep reactions can be combined in a single step in one pot by optimizing multistep reactions under identical reaction condition. Using this analogy, the process of making PEGylated insulin, IN-105, was simplified. Instead of taking the purified active insulin bulk powder as the starting mat...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-012-0722-4
更新日期：2012-10-01 00:00:00
abstract：:Recycled paper mills produce large quantities of fibrous rejects and fines which are usually sent to landfills as solid waste. These cellulosic materials can be enzymatically hydrolyzed into sugars for the production of biofuels and biomaterials. Paper mill wastes also contain large amounts of calcium carbonate which ...
journal_title：Bioprocess and biosystems engineering
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更新日期：2017-06-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
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更新日期：2018-06-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
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更新日期：2013-04-01 00:00:00
abstract：:The discovery of quorum sensing as a mechanism for regulating specific phenotypes in bacteria based on population density has conveyed attention to find molecules capable of interfering quorum sensing networks (QSN) in a process coined quorum quenching. Here, we examined the dynamics of Escherichia coli AI-2 and Pseud...
journal_title：Bioprocess and biosystems engineering
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更新日期：2009-06-01 00:00:00
abstract：:Oxygen supply is crucial in industrial application of microbial systems, such as Corynebacterium glutamicum, but oxygen transfer is often neglected in early strain characterizations, typically done under aerobic conditions. In this work, a new procedure for oxygen transfer screening is presented, assessing the impact ...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-014-1234-1
更新日期：2014-12-01 00:00:00
abstract：:tert-Butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate [(3R,5S)-CDHH] is the key chiral intermediate to synthesize the side chain of the lipid-lowering drug rosuvastatin. Carbonyl reductases showed excellent activity for the biosynthesis of (3R,5S)-CDHH. The requirement of cofactor NADH/NADPH leads to high cost for the in...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-019-02201-x
更新日期：2020-01-01 00:00:00
abstract：:In this study, citric acid production from extract of Jerusalem artichoke tubers by the genetically engineered yeast Yarrowia lipolytica strain 30 was investigated. After the compositions of the extract of Jerusalem artichoke tubers for citric acid production were optimized, the results showed that natural components ...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-013-0951-1
更新日期：2013-11-01 00:00:00
abstract：:Escherichia coli AFP111, a pflB, ldhA, ptsG triple mutant of E. coli W1485, can be recovered for additional succinate production in fresh medium after two-stage fermentation (an aerobic growth stage followed by an anaerobic production stage). However, the specific productivity is lower than that of two-stage fermentat...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-010-0484-9
更新日期：2011-05-01 00:00:00
abstract：:Recently, several studies have been published on the application of plasmid DNA (pDNA) in gene therapy and vaccine production. The bioprocess to obtain pDNA involves the steps of fermentation, primary recovery, secondary recovery and final purification. The pDNA primary recovery, which is the key step to the rest of t...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-015-1351-5
更新日期：2015-06-01 00:00:00
abstract：:Lignin-modifying enzymes have various promising applications such as biobleaching, biopulping, the functionalization of lignocellulosic materials, the modification of wood fibers, the remediation of contaminated soil and effluents, as well as improvement of the enzymatic hydrolysis of lignocellulosic substrates. In th...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-014-1243-0
更新日期：2015-01-01 00:00:00
abstract：:In this study, Escherichia coli cells overexpressing lysine decarboxylase (CadA) were used for cadaverine production. Barium alginate was selected as a matrix for immobilization of E. coli YH91. Free cells and immobilized cells (IC) were characterized for their physiochemical properties, and the optimum pH and tempera...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-015-1465-9
更新日期：2015-12-01 00:00:00
