当前位置： SCI文献检索 > BIOPROCESS AND BIOSYSTEMS ENGINEERING期刊下所有文献 > Asymmetric synthesis of tert-butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate using a self-sufficient biocatalyst based on carbonyl reductase and cofactor co-immobilization.

Asymmetric synthesis of tert-butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate using a self-sufficient biocatalyst based on carbonyl reductase and cofactor co-immobilization.

Abstract：

:tert-Butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate [(3R,5S)-CDHH] is the key chiral intermediate to synthesize the side chain of the lipid-lowering drug rosuvastatin. Carbonyl reductases showed excellent activity for the biosynthesis of (3R,5S)-CDHH. The requirement of cofactor NADH/NADPH leads to high cost for the industrial application of carbonyl reductases. In this study, a self-sufficient biocatalyst based on carbonyl reductase and NADP+ co-immobilization strategy was developed on an amino resin carrier LX-1000HAA (SCR-NADP+@LX-1000HAA). The self-sufficient biocatalyst achieved in situ cofactor regeneration and showed the activity recovery of 77.93% and the specific activity of 70.45 U/g. Asymmetric synthesis of (3R,5S)-CDHH using SCR-NADP+@LX-1000HAA showed high enantioselectivity (> 99% e.e.) and yield (98.54%). Batch reactions were performed for ten cycles without extra addition of NADP+, and the total yield of (3R,5S)-CDHH achieved at 10.56 g/g biocatalyst. The present work demonstrated the potential of the self-sufficient biocatalyst for the asymmetric biosynthesis of rosuvastatin intermediate.

journal_name

Bioprocess Biosyst Eng

journal_title

Bioprocess and biosystems engineering

authors

Zhang XJ,Wang WZ,Zhou R,Liu ZQ,Zheng YG

doi

10.1007/s00449-019-02201-x

subject

Has Abstract

pub_date

2020-01-01 00:00:00

pages

21-31

issue

1

eissn

1615-7591

issn

1615-7605

pii

10.1007/s00449-019-02201-x

journal_volume

43

pub_type

杂志文章

相关文献

BIOPROCESS AND BIOSYSTEMS ENGINEERING文献大全
  • Reaction rate constants and mean population percentage for nitrifiers in an alternating oxidation ditch system.

    abstract：:This paper presents a methodology for the determination of reaction rate constants for nitrifying bacteria and their mean population percentage in biomass in an alternating oxidation ditch system. The method used is based on the growth rate equations of the ASM1 model (IWA) (Henze et al. in Activated sludge models ASM...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-010-0446-2

    authors： Mantziaras ID,Katsiri A

    更新日期：2011-01-01 00:00:00

  • Investigation of the reversibility of freeze/thaw stress-induced protein instability using heat cycling as a function of different cryoprotectants.

    abstract：:Formulation conditions have a significant influence on the degree of freeze/thaw (FT) stress-induced protein instabilities. Adding cryoprotectants might stabilize the induced FT stress instabilities. However, a simple preservation of protein stability might be insufficient and further methods are necessary. This study...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-020-02327-3

    authors： Wöll AK,Hubbuch J

    更新日期：2020-07-01 00:00:00

  • Population heterogeneity in microbial bioprocesses: origin, analysis, mechanisms, and future perspectives.

    abstract：:Population heterogeneity is omnipresent in all bioprocesses even in homogenous environments. Its origin, however, is only so well understood that potential strategies like bet-hedging, noise in gene expression and division of labour that lead to population heterogeneity can be derived from experimental studies simulat...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章,评审

    doi：10.1007/s00449-018-1922-3

    authors： Heins AL,Weuster-Botz D

    更新日期：2018-07-01 00:00:00

  • Broad-spectrum electrochemical immunosensor based on one-step electrodeposition of AuNP-Abs and Prussian blue nanocomposite for organophosphorus pesticide detection.

    abstract：:Broad-spectrum antibodies can effectively recognize substances with similar structures and have broad application prospects in field rapid detection. In this study, broad-spectrum antibodies (Abs) against organophosphorus pesticides (OPs) were used as sensitive recognition elements, which could effectively recognize m...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-020-02472-9

    authors： Dong H,Zhao Q,Li J,Xiang Y,Liu H,Guo Y,Yang Q,Sun X

    更新日期：2020-11-08 00:00:00

  • In situ synthesis of polypyrrole on graphite felt as bio-anode to enhance the start-up performance of microbial fuel cells.

    abstract：:This study introduces an effective method to deposit polypyrrole (PPy) on graphite felt (GF) as anode to improve the start-up performance of microbial fuel cells (MFCs). The results of scanning electron microscope (SEM) and electrochemical testing reveal that polypyrrole is able to improve the electrical conductivity ...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-019-02238-y

    authors： Pu KB,Lu CX,Zhang K,Zhang H,Chen QY,Wang YH

    更新日期：2020-03-01 00:00:00

  • Innovative methods to generate clean sugar stream from biomass feedstocks for efficient fermentation.

    abstract：:Sorghum stalks were evaluated to generate clean sugars; enzymatic hydrolysis efficiency of acid pretreated biomass was significantly low (12.3 g sugar/g pretreated biomass) compared to alkali method (49.1 g sugar/g pretreated biomass). Inhibitory compounds derived from acid pretreatment, such as hydroxymethyl furfural...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章,评审

    doi：10.1007/s00449-016-1727-1

    authors： Lee JE,Guragain YN,Bastola KP,Vadlani PV

    更新日期：2017-04-01 00:00:00

  • Comprehensive assessment of the L-lysine production process from fermentation of sugarcane molasses.

    abstract：:L-Lysine is an essential amino acid that can be produced by chemical processes from fossil raw materials, as well as by microbial fermentation, the latter being a more efficient and environmentally friendly procedure. In this work, the production process of L-lysine-HCl is studied using a systematic approach based on ...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-017-1766-2

    authors： Anaya-Reza O,Lopez-Arenas T

    更新日期：2017-07-01 00:00:00

  • Enzymatic hydrolysis of cellulose in a membrane bioreactor: assessment of operating conditions.

    abstract：:The optimization of operating conditions for cellulose hydrolysis was systemically undertaken using an ultra-scaled down membrane bioreactor based on the parameter scanning ultrafiltration apparatus. The bioconversion of cellulose saccharification was carried out with freely suspended cellulase from Aspergillus niger ...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-010-0501-z

    authors： Liu J,Lu J,Cui Z

    更新日期：2011-06-01 00:00:00

  • Highly efficient synthesis of boldenone from androst-4-ene-3,17-dione by Arthrobacter simplex and Pichia pastoris ordered biotransformation.

    abstract：:Boldenone (BD) is an important steroid hormone drug which is the derivative of testosterone. In this study, an ordered biotransformation method was proposed employing Arthrobacter simplex and recombinant Pichia pastoris with 17β-hydroxysteroid dehydrogenase from Saccharomyces cerevisiae to produce BD from androst-4-en...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-019-02092-y

    authors： Tang R,Shen Y,Wang M,Zhou H,Zhao Y

    更新日期：2019-06-01 00:00:00

  • Effect of pH on the metabolic flux of Klebsiella oxytoca producing 2,3-butanediol in continuous cultures at different dilution rates.

    abstract：:The efficiency of the bioconversion process and the achievable end-product concentration decides the economic feasibility of microbial 2,3-butanediol (2,3-BDO) production. In 2,3-BDO production, optimization of culture condition is required for cell growth and metabolism. Also, the pH is an important factor that influ...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-013-0932-4

    authors： Park C,Lu M,Yun S,Park K,Lee J

    更新日期：2013-06-01 00:00:00

  • Construction of a high efficiency copper adsorption bacterial system via peptide display and its application on copper dye polluted wastewater.

    abstract：:For the construction of an efficient copper waste treatment system, a cell surface display strategy was employed. The copper adsorption ability of recombinant bacterial strains displaying three different copper binding peptides were evaluated in LB Luria-Bertani medium (LB), artificial wastewater, and copper phthalocy...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-015-1447-y

    authors： Maruthamuthu MK,Nadarajan SP,Ganesh I,Ravikumar S,Yun H,Yoo IK,Hong SH

    更新日期：2015-11-01 00:00:00

  • Preparation and synthetic dye decolorization ability of magnetic cross-linked enzyme aggregates of laccase from Bacillus amyloliquefaciens.

    abstract：:Laccases are versatile oxidases that are capable of decolorizing various synthetic dyes. Recombinant Bacillus amyloliquefaciens laccase was immobilized as magnetic cross-linked enzyme aggregates (M-CLEAs) for application in dye decolorization. Several parameters influencing the activity recovery were evaluated during ...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-020-02481-8

    authors： Wang H,Han S,Wang J,Yu S,Li X,Lu L

    更新日期：2020-11-27 00:00:00

  • A metabolic network-based approach for developing feeding strategies for CHO cells to increase monoclonal antibody production.

    abstract：:Chinese hamster ovary (CHO) cells are the main workhorse in the biopharmaceutical industry for the production of recombinant proteins, such as monoclonal antibodies. To date, a variety of metabolic engineering approaches have been used to improve the productivity of CHO cells. While genetic manipulations are potential...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-020-02332-6

    authors： Fouladiha H,Marashi SA,Torkashvand F,Mahboudi F,Lewis NE,Vaziri B

    更新日期：2020-08-01 00:00:00

  • Biotransformation of lysine into cadaverine using barium alginate-immobilized Escherichia coli overexpressing CadA.

    abstract：:In this study, Escherichia coli cells overexpressing lysine decarboxylase (CadA) were used for cadaverine production. Barium alginate was selected as a matrix for immobilization of E. coli YH91. Free cells and immobilized cells (IC) were characterized for their physiochemical properties, and the optimum pH and tempera...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-015-1465-9

    authors： Bhatia SK,Kim YH,Kim HJ,Seo HM,Kim JH,Song HS,Sathiyanarayanan G,Park SH,Park K,Yang YH

    更新日期：2015-12-01 00:00:00

  • Combining De Ley-Doudoroff and methylerythritol phosphate pathways for enhanced isoprene biosynthesis from D-galactose.

    abstract：:An engineered Escherichia coli strain was developed for enhanced isoprene production using D-galactose as substrate. Isoprene is a valuable compound that can be biosynthetically produced from pyruvate and glyceraldehyde-3-phosphate (G3P) through the methylerythritol phosphate pathway (MEP). The Leloir and De Ley-Doudo...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-014-1228-z

    authors： Ramos KR,Valdehuesa KN,Liu H,Nisola GM,Lee WK,Chung WJ

    更新日期：2014-12-01 00:00:00

  • Surfactant removal from wastewater using photo-cathode microbial fuel cell and laterite-based hybrid treatment system.

    abstract：:Sodium dodecyl sulfate (SDS) is a widely used anionic surfactant, which finds its way to the receiving water body due to the incapability of conventional wastewater treatment systems to completely remove it. A hybrid treatment system consisting of upflow microbial fuel cell (MFC) with titanium dioxide (TiO2) as a phot...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-020-02396-4

    authors： Sathe SM,Bhowmick GD,Dubey BK,Ghangrekar MM

    更新日期：2020-11-01 00:00:00

  • Whole slurry fermentation of maleic acid-pretreated oil palm empty fruit bunches for ethanol production not necessitating a detoxification process.

    abstract：:The yield of ethanol from oil palm empty fruit bunches (EFB) was increased on exploiting maleic acid pretreatment combined with fermentation of the pretreated whole slurry. The optimized conditions for pretreatment were to expose EFB to a high temperature (190 °C) with 1 % (w/v) maleic acid for a short time duration (...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-013-1035-y

    authors： Jung YH,Kim IJ,Kim HK,Kim KH

    更新日期：2014-04-01 00:00:00

  • Influence of photobioreactor configuration on microalgal biomass production.

    abstract：:Biodiesel production from microalgae depends on the biomass concentration and lipid content in microalgal cells. Photobioreactors (PBRs) facilitates cultivation of microalgae and renders better process control than open systems. However, reactor configuration and consequential hydrodynamics considerably influence biom...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-020-02342-4

    authors： Banerjee S,Dasgupta S,Das D,Atta A

    更新日期：2020-08-01 00:00:00

  • Magneto-controlled bioelectronics for the antigen-antibody interaction based on magnetic-core/gold-shell nanoparticles functionalized biomimetic interface.

    abstract：:A new protein assay system for the antigen-antibody interaction was developed by immobilization of carcinoembryonic antibody (anti-CEA) onto magnetic-core/gold-shell nanoparticles-functionalized biomimetic interface on multiporous polythionine modified magnetic carbon paste electrodes (MCPE). Differential pulse voltam...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-007-0145-9

    authors： Tang D,Yuan R,Chai Y

    更新日期：2008-02-01 00:00:00

  • Evaluation of sludge reduction by an environmentally friendly chemical uncoupler in a pilot-scale anaerobic/anoxic/oxic process.

    abstract：:An environmentally friendly chemical, tetrakis(hydroxymethyl)phosphonium sulfate (THPS), was used as a metabolic uncoupler to reduce sludge production in a pilot-scale anaerobic/anoxic/oxic process. The results show that the addition of THPS (1.08-1.86 mL/m(3) influent) in the sludge return section could reduce waste ...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-013-1022-3

    authors： Guo X,Yang J,Liang Y,Liu J,Xiao B

    更新日期：2014-03-01 00:00:00

  • Biomimetic oxidase sensor based on functionalized surface of carbon nanotubes and iron prophyrins for catechol detection.

    abstract：:A novel and highly stable biomimetic oxidase sensor system was designed for catehol detection. FePP used as biomimetic horseradish peroxidase (HRP) was immobilized onto modified multi-walled carbon nanotubes (MWCNTs). Functional groups such as -OH, -NH2 and -COOH were introduced onto the surface of MWCNTs to provide b...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-018-2032-y

    authors： Bin Z,Yanhong C,Jiaojiao X

    更新日期：2019-02-01 00:00:00

  • Effect of visible light on catalytic hydrolysis of p-nitrophenyl palmitate by the Pseudomonas cepacia lipase immobilized on sol-gel support.

    abstract：:This paper demonstrates Pseudomonas cepacia lipase catalyzed hydrolysis of p-nitrophenyl palmitate under irradiation of light with wavelengths of 250-750 nm. The reaction follows Michaelis-Menten Kinetics and the light irradiation increases the overall rate of hydrolysis. Using Lineweaver-Burk plot K M and V max value...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-014-1213-6

    authors： Ganasen P,Khan MR,Kalam MA,Mahmud MS

    更新日期：2014-11-01 00:00:00

  • Mass transfer characterization of gamma-aminobutyric acid production by Enterococcus faecium CFR 3003: encapsulation improves its survival under simulated gastro-intestinal conditions.

    abstract：:Gamma-aminobutyric acid (GABA) production by free and Ca-alginate encapsulated cells of Enterococcus faecium CFR 3003 was investigated. Mass transfer rates characterizing the GABA production process using encapsulated cells were investigated. Experiments were performed to investigate external film and internal pore di...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-014-1296-0

    authors： Divyashri G,Prapulla SG

    更新日期：2015-03-01 00:00:00

  • Probing control of glucose feeding in Vibrio cholerae cultivations.

    abstract：:Infection with Vibrio cholerae is a significant problem in many developing countries. Cultivation of V. cholerae is used in production of cholera toxin B subunit, which is a component in a cholera vaccine. Fed-batch cultivations with V. cholerae in defined media have been conducted and reproducible results were obtain...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-002-0304-y

    authors： de Maré L,Andersson L,Hagander P

    更新日期：2003-01-01 00:00:00

  • Covalent immobilization of Kluyveromyces fragilis β-galactosidase on magnetic nanosized epoxy support for synthesis of galacto-oligosaccharide.

    abstract：:The novel magnetic nanobeads with epoxy groups on the surface were prepared from glycidyl methacrylate (GMA), ethylene glycol dimethacrylate (EGDMA) and hydroxyethyl methacrylate (HEMA) via emulsifier-free emulsion polymerisation, and they were characterized by scanning electron microscopy and vibrating sample magneto...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-012-0716-2

    authors： Liu H,Liu J,Tan B,Zhou F,Qin Y,Yang R

    更新日期：2012-10-01 00:00:00

  • Influence of oxygen transfer on Pseudomonas putida effects on growth rate and biodesulfurization capacity.

    abstract：:The growth rate and desulfurization capacity accumulated by the cells during the growth of Pseudomonas putida KTH2 under different oxygen transfer conditions in a stirred and sparged tank bioreactor have been studied. Hydrodynamic conditions were changed using different agitation conditions. During the culture, severa...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-016-1536-6

    authors： Escobar S,Rodriguez A,Gomez E,Alcon A,Santos VE,Garcia-Ochoa F

    更新日期：2016-04-01 00:00:00

  • Prevalence of Nitrosomonas cluster 7 populations in the ammonia-oxidizing community of a submerged membrane bioreactor treating urban wastewater under different operation conditions.

    abstract：:A pilot-scale ultrafiltration membrane bioreactor (MBR) was used for the aerobic treatment of urban wastewater in four experimental stages influenced by seasonal temperature and different sets of operation conditions. The structure of the ammonia-oxidizing bacteria (AOB) community was profiled by temperature gradient ...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-012-0823-0

    authors： Cerrone F,Poyatos JM,Molina-Muñoz M,Cortés-Lorenzo C,González-López J,Rodelas B

    更新日期：2013-07-01 00:00:00

  • A new method for immobilization of acetylcholinesterase.

    abstract：:A new method for immobilization of acetylcholinesterase (AChE) to alginate gel beads by activating the carbonyl groups of alginate using carbodiimide coupling agent has been successfully developed. Maximum reaction rate (V (max)) and Michaelis-Menten constant (K (m)) were determined for the free and binary immobilized...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-006-0106-8

    authors： Tümtürk H,Sahin F,Demirel G

    更新日期：2007-03-01 00:00:00

  • Oxidases from mate tea leaves (Ilex paraguariensis): extraction optimization and stability at low and high temperatures.

    abstract：:Mate (Ilex paraguariensis) is an important natural product in the economic and cultural context of Brazil. Peroxidase and polyphenol oxidase have been responsible for quality deterioration and browning in mate. The objective of this work was to investigate a methodology of extraction and enzymatic activity determinati...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-007-0196-y

    authors： Ceni GC,Baldissera EM,Antunes OA,Vladimir Oliveira J,Dariva C,de Oliveira D

    更新日期：2008-10-01 00:00:00

  • Comparison of sulfuric and hydrochloric acids as catalysts in hydrolysis of Kappaphycus alvarezii (cottonii).

    abstract：:In this study, hydrolysis of marine algal biomass Kappaphhycus alvarezii using two different acid catalysts was examined with the goal of identifying optimal reaction conditions for the formation of sugars and by-products. K. alvarezii were hydrolyzed by autoclave using sulfuric acid or hydrochloric acid as catalyst w...

    journal_title：Bioprocess and biosystems engineering

    pub_type： 杂志文章

    doi：10.1007/s00449-011-0609-9

    authors： Meinita MD,Hong YK,Jeong GT

    更新日期：2012-01-01 00:00:00