Abstract：
:Mate (Ilex paraguariensis) is an important natural product in the economic and cultural context of Brazil. Peroxidase and polyphenol oxidase have been responsible for quality deterioration and browning in mate. The objective of this work was to investigate a methodology of extraction and enzymatic activity determination of oxidases present in mate tea leaves and to evaluate the oxidases stability. The effects of raw-material mass, buffer molar concentration, Triton X-100 addition, extraction pH, pH activity measurement, polyvinylpyrrolidone K90 addition, and centrifugation time were evaluated by the experimental planning methodology. The storage of the oxidases along 150 days at low temperatures showed that no significant difference was found at -4 and -80 degrees C but significant difference was observed when compared to 4 degrees C. The results showed that higher activities of oxidases are obtained at similar conditions. The exposition to high-temperatures and the variation of the time of exposition affected the enzymatic activity significantly.
journal_name
Bioprocess Biosyst Engjournal_title
Bioprocess and biosystems engineeringauthors
Ceni GC,Baldissera EM,Antunes OA,Vladimir Oliveira J,Dariva C,de Oliveira Ddoi
10.1007/s00449-007-0196-ysubject
Has Abstractpub_date
2008-10-01 00:00:00pages
541-50issue
6eissn
1615-7591issn
1615-7605journal_volume
31pub_type
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