Highly selective resonance scattering detection of trace thrombin using aptamer-modified AuRe nanoprobe.

abstract：:The phasing technique is a method for synchronizing cell populations in a bioreactor. Periodic changes of substrate supply and depletion can provoke a cell cycle phasing of originally stochastic scattered proliferation patterns. Synchronized cell populations characterized by changes in DNA content distribution can be ...

journal_title：Bioprocess and biosystems engineering

authors： Noack S,Klöden W,Bley T

更新日期：2008-08-01 00:00:00

abstract：:In this study, hydrolysis of marine algal biomass Kappaphhycus alvarezii using two different acid catalysts was examined with the goal of identifying optimal reaction conditions for the formation of sugars and by-products. K. alvarezii were hydrolyzed by autoclave using sulfuric acid or hydrochloric acid as catalyst w...

journal_title：Bioprocess and biosystems engineering

authors： Meinita MD,Hong YK,Jeong GT

更新日期：2012-01-01 00:00:00

abstract：:This work aimed to develop an optimal carbon source feeding strategy to achieve maximal production of heparosan as a precursor of bioengineered heparin by Escherichia coli K5. Glycerol gave higher heparosan titer and productivity compared to glucose. The maximum heparosan production (187 mg/L) and heparosan productivi...

journal_title：Bioprocess and biosystems engineering

authors： Liu Y,Liu L,Chen J,Li J,Du G,Chen J

更新日期：2012-09-01 00:00:00

abstract：:Menaquinone homologs (MK-n), that is, MK-4, MK-5, and MK-6, can be produced by the fermentation of Flavobacterium. In this study, we proposed a simple and efficient method for the extraction of menaquinones from wet cells without the process of drying the biomass. Meanwhile, a rapid and effective solution for the sepa...

journal_title：Bioprocess and biosystems engineering

authors： Wei H,Zhao G,Liu H,Wang H,Ni W,Wang P,Zheng Z

更新日期：2018-01-01 00:00:00

abstract：:Pullulanase was extracellularly produced with an engineered Escherichia coli with a combined strategy. When auto-induction instead of isopropyl β-D-1-thiogalactopyranoside (IPTG) induction method was implemented, we observed increased extracellular activity (4.2 U ml(-1)) and cell biomass (7.95 g DCW l(-1)). Subsequen...

journal_title：Bioprocess and biosystems engineering

authors： Chen WB,Nie Y,Xu Y,Xiao R

更新日期：2014-04-01 00:00:00

abstract：:Broad-spectrum antibodies can effectively recognize substances with similar structures and have broad application prospects in field rapid detection. In this study, broad-spectrum antibodies (Abs) against organophosphorus pesticides (OPs) were used as sensitive recognition elements, which could effectively recognize m...

journal_title：Bioprocess and biosystems engineering

authors： Dong H,Zhao Q,Li J,Xiang Y,Liu H,Guo Y,Yang Q,Sun X

更新日期：2020-11-08 00:00:00

abstract：:An new cascade control system is presented that reproducibly keeps the cultivation part of recombinant protein production processes on its predetermined track. While the system directly controls carbon dioxide production mass and carbon dioxide production rates along their setpoint profiles in fed-batch cultivation, i...

journal_title：Bioprocess and biosystems engineering

authors： Kuprijanov A,Schaepe S,Aehle M,Simutis R,Lübbert A

更新日期：2012-03-01 00:00:00

abstract：:Horseradish peroxidase (HRP) was immobilized on the polyaniline (PANI) grafted polyacrylonitrile (PAN) films. The maximum HRP immobilization capacity of the PAN-g-PANI-3 film was 221 μg/cm(2). The HRP-immobilized PAN-g-PANI-3 film retained 79 % of the activity of the same quantity free enzyme. The HRP-immobilized PAN-...

journal_title：Bioprocess and biosystems engineering

authors： Bayramoglu G,Altintas B,Yakup Arica M

更新日期：2012-10-01 00:00:00

abstract：:The aim of this study is to achieve high-level extracellular production of D-Psicose-3-epimerase (DPE) with recombinant Escherichia coli. High-level production of DPE is one of the key factors in D-Psicose production. In the present study, the gene AAL45544.1 from Agrobacterium tumefaciens str. C58 was modified by art...

journal_title：Bioprocess and biosystems engineering

authors： Gu L,Zhang J,Liu B,Wu C,Du G,Chen J

更新日期：2013-11-01 00:00:00

abstract：:A Candida rugosa lipase immobilized on polypropylene powder was employed as the biocatalyst for the enantioselective hydrolysis of (R, S)-suprofen 2,2,2-trifluorothioester in cyclohexane, in which trioctylamine was added as the catalyst to perform in situ racemization of the remaining (R)-thioester. A hollow-fiber mem...

journal_title：Bioprocess and biosystems engineering

authors： Wang LW,Cheng YC,Tsai SW

更新日期：2004-12-01 00:00:00

abstract：:Bacteria from Patagonian Merino wool were isolated to assess their wool-keratinolytic activity and potential for felt-resist treatments. Strains from Bacillus, Exiguobacterium, Deinococcus, and Micrococcus produced wool-degrading enzymes. Bacillus sp. G51 showed the highest wool-keratinolytic activity. LC-MS/MS analys...

journal_title：Bioprocess and biosystems engineering

authors： Iglesias MS,Sequeiros C,García S,Olivera NL

更新日期：2017-06-01 00:00:00

abstract：:Lactococcus lactis spp. lactis and Lactococcus lactis spp. cremoris are widely used in the manufacture of fermented milk. These strains were compared for production of Dipeptidyl Peptidase IV (DPP IV) enzyme in terms of enzyme activity, specific growth rates and productivity. Lactococcus lactis spp. lactis was produce...

journal_title：Bioprocess and biosystems engineering

authors： Ustün O,Ongen G

更新日期：2012-10-01 00:00:00

abstract：:The bacteriostatic properties of carbon disulphide (CS₂) hamper its biodegradation in conventional biofilters. The response of four biofilters operating in downflow mode and reverse-flow mode was compared in a laboratory-scale plant treating CS₂ under sudden short-term changes in operating conditions. A process shutdo...

journal_title：Bioprocess and biosystems engineering

authors： Rojo N,Gallastegui G,Gurtubay L,Barona A,Elías A

更新日期：2013-04-01 00:00:00

abstract：:Chinese hamster ovary (CHO) cells are the main workhorse in the biopharmaceutical industry for the production of recombinant proteins, such as monoclonal antibodies. To date, a variety of metabolic engineering approaches have been used to improve the productivity of CHO cells. While genetic manipulations are potential...

journal_title：Bioprocess and biosystems engineering

authors： Fouladiha H,Marashi SA,Torkashvand F,Mahboudi F,Lewis NE,Vaziri B

更新日期：2020-08-01 00:00:00

abstract：:Broken rice, pretreated by enzymatic extrusion liquefaction, was used to produce Chinese rice wine by simultaneous saccharification and fermentation (SSF) process in this study. The study compared the novel process and traditional process for Chinese rice wine fermentation utilizing broken rice and head rice, respecti...

journal_title：Bioprocess and biosystems engineering

authors： Li H,Jiao A,Xu X,Wu C,Wei B,Hu X,Jin Z,Tian Y

更新日期：2013-08-01 00:00:00

abstract：:This work is focused on the characterization of a commercial cellulase in terms of optimum pH and temperature, stability to pH and temperature and affinity of this enzyme to several substrates, determining the Michaelis-Menten parameters. Maximum activity of cellulase was obtained for the temperature range from 40 to ...

journal_title：Bioprocess and biosystems engineering

authors： Balsan G,Astolfi V,Benazzi T,Meireles MA,Maugeri F,Di Luccio M,Dal Prá V,Mossi AJ,Treichel H,Mazutti MA

更新日期：2012-09-01 00:00:00

abstract：:The previous works on simulated broths are continued and developed for Propionibacterium shermanii broths. The obtained results indicated the considerable increase of kLa in presence of n-dodecane as oxygen-vector and the existence of a certain value of hydrocarbon concentration that corresponds to the maximum mass tr...

journal_title：Bioprocess and biosystems engineering

authors： Galaction AI,Cascaval D,Turnea M,Folescu E

更新日期：2005-07-01 00:00:00

abstract：:Fibrinolytic proteases are enzymes that degrade fibrin. They provide a promising alternative to existing drugs for thrombolytic therapy. A protease isolated from the filamentous fungus Mucor subtilissimus UCP 1262 was purified in three steps by ammonium sulfate fractionation, ion exchange, and molecular exclusion chro...

journal_title：Bioprocess and biosystems engineering

authors： Nascimento TP,Sales AE,Porto TS,Costa RMPB,Breydo L,Uversky VN,Porto ALF,Converti A

更新日期：2017-08-01 00:00:00

abstract：:The effect of a well-defined mild shear stress on hybridoma cell viability (HB-8852) in a serum-free culture medium has been analysed, and the role as shear protector of different concentrations of fetal bovine serum have been studied. Samples harvested from cultures in their late exponential growth phase, were subjec...

journal_title：Bioprocess and biosystems engineering

authors： Legazpi L,Laca A,Díaz M

更新日期：2009-10-01 00:00:00

abstract：:A novel and efficient facultative anaerobic denitrifying bacterium was isolated and identified as Pseudomonas citronellolis WXP-4. The strain WXP-4 could achieve 100% nitrate and nitrite removal efficiency utilizing sodium succinate as a carbon source, C/N ratio 7, pH 7.0, and temperature 40 °C under both aerobic and ...

journal_title：Bioprocess and biosystems engineering

authors： Wang X,Duo Y,He J,Yao J,Qian H,Hrynsphan D,Tatsiana S,Chen J

更新日期：2020-05-01 00:00:00

abstract：:We propose an adaptive control law that allows one to identify unstable steady states of the open-loop system in the single-species chemostat model without the knowledge of the growth function. We then show how one can use this control law to trace out (reconstruct) the whole graph of the growth function. The process ...

journal_title：Bioprocess and biosystems engineering

authors： Sieber J,Rapaport A,Rodrigues S,Desroches M

更新日期：2013-10-01 00:00:00

abstract：:The biological production of butanol has become an important research field and thanks to genome sequencing and annotation; genome-scale metabolic reconstructions have been developed for several Clostridium species. This work makes use of the iCAC490 model of Clostridium acetobutylicum ATCC 824 to analyze its metaboli...

journal_title：Bioprocess and biosystems engineering

authors： Gallardo R,Acevedo A,Quintero J,Paredes I,Conejeros R,Aroca G

更新日期：2016-02-01 00:00:00

abstract：:The aim of this study was to investigate the efficacy of a hybrid biofilm pilot-scale treatment plant, designed with a novel configuration by the integration of a fixed-film system, to improve nitrogen removal. The pilot-scale system was established at a wastewater treatment plant in Istanbul and operated based on str...

journal_title：Bioprocess and biosystems engineering

authors： Güneş G,Hallaç E,Özgan M,Ertürk A,Okutman Taş D,Çokgor E,Güven D,Takacs I,Erdinçler A,Insel G

更新日期：2019-03-01 00:00:00

abstract：:Colony development of the dimorphic yeasts Yarrowia lipolytica and Candida boidinii on solid agar substrates under glucose limitation served as a model system for mycelial development of higher filamentous fungi. Strong differences were observed in the behaviour of both yeasts: C. boidinii colonies reached a final col...

journal_title：Bioprocess and biosystems engineering

authors： Walther T,Reinsch H,Weber P,Ostermann K,Deutsch A,Bley T

更新日期：2011-01-01 00:00:00

abstract：:Of various metal ions (Ca2+, Cr3+, Cu2+, Fe2+, Mg2+, Mn2+, Ni2+ and Zn2+) added to the culture medium of Agrobacterium tumefaciens at 1 mM, only Ca2+ increased Coenzyme Q10 (CoQ(10)) content in cells without the inhibition of cell growth. In a pH-stat fed-batch culture, supplementation with 40 mM of CaCO3 increased th...

journal_title：Bioprocess and biosystems engineering

authors： Ha SJ,Kim SY,Seo JH,Jeya M,Zhang YW,Ramu T,Kim IW,Lee JK

更新日期：2009-08-01 00:00:00

abstract：:Two laboratory-scale expanded granular sludge bed (EGSB) reactors were operated at 18 and 25 °C, respectively, for the treatment of synthetic wastewater composed of ethanol and 1-methoxy-2-propanol (M2P) in a mass ratio of 4:1. Reactors were operated first with continuous wastewater supply and after with discontinuous...

journal_title：Bioprocess and biosystems engineering

authors： Lafita C,Penya-roja JM,Gabaldón C

更新日期：2015-11-01 00:00:00

abstract：:Unfolded protein response (UPR) usually happens when expressing heterologous proteins in high level, which may help cells to facilitate protein processing. Here, we evaluated the effects of the UPR activator HAC1p on a raw-starch hydrolyzing α-amylase (Gs4j-amyA), so as to improve heterologous production of the enzyme...

journal_title：Bioprocess and biosystems engineering

authors： Huang M,Gao Y,Zhou X,Zhang Y,Cai M

更新日期：2017-03-01 00:00:00

abstract：:The hydrolase (Thermobifida fusca hydrolase; TfH) from T. fusca was produced in Escherichia coli as fusion protein using the OmpA leader sequence and a His(6) tag. Productivity could be raised more than 100-fold. Both batch and fed-batch cultivations yield comparable cell specific productivities whereas volumetric pro...

journal_title：Bioprocess and biosystems engineering

authors： Dresler K,van den Heuvel J,Müller RJ,Deckwer WD

更新日期：2006-08-01 00:00:00

abstract：:Candida cylindracea NRRL Y-17506 was grown to produce extracellular lipase from oleic acid as a carbon source. Through flask cultures, it was found that the optimum initial oleic acid concentration for cell growth was 20 g l(-1). However, high initial concentrations of oleic acid up to 50 g l(-1) were not inhibitory. ...

journal_title：Bioprocess and biosystems engineering

authors： Kim BS,Hou CT

更新日期：2006-06-01 00:00:00

abstract：:Recently, several studies have been published on the application of plasmid DNA (pDNA) in gene therapy and vaccine production. The bioprocess to obtain pDNA involves the steps of fermentation, primary recovery, secondary recovery and final purification. The pDNA primary recovery, which is the key step to the rest of t...

journal_title：Bioprocess and biosystems engineering

authors： Padilla-Zamudio A,Guerrero-Germán P,Tejeda-Mansir A

更新日期：2015-06-01 00:00:00