Abstract：
:Industrial grade soluble corn starch was used directly and effectively as the fermentation substrate for microbial exopolysaccharides production. Bacillus subtilis mutant strain NJ308 grew with untreated starch raw material as the sole carbon source. The real-time PCR results demonstrated that up-regulated genes encoding N-acetylglucosaminyltransferase, mannosyltransferase, and N-acetylglucosamine-1-phosphate uridyltransferase were the key elements of B. subtilis mutant strain NJ308 for exopolysaccharides production from industrial grade starch. Subsequently, the culture conditions for B. subtilis NJ308 were optimized using Plackett-Burman design and central composite design methods, and the related key genes in the synthesis pathway of exopolysaccharides from the starch raw material were analyzed by real-time PCR. The maximum exopolysaccharides titration (3.41 g/L) was obtained when the initial starch concentration was 45 g/L. This corresponds to volumetric productivity values of 71.04 mg/L h.
journal_name
Bioprocess Biosyst Engjournal_title
Bioprocess and biosystems engineeringauthors
He X,He F,Hang J,Li H,Chen Y,Wei P,Chen K,Li Y,OuYang Pdoi
10.1007/s00449-018-1915-2subject
Has Abstractpub_date
2018-06-01 00:00:00pages
811-817issue
6eissn
1615-7591issn
1615-7605pii
10.1007/s00449-018-1915-2journal_volume
41pub_type
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