Abstract:
:A novel β-1,3-1,4-glucanase gene was identified in Bacillus sp. SJ-10 (KCCM 90078) isolated from jeotgal, a traditional Korean fermented fish. We analysed the β-1,3-1,4-glucanase gene sequence and examined the recombinant enzyme. The open reading frame of the gene encoded 244 amino acids. The sequence was not identical to any β-glucanases deposited in GenBank. The gene was cloned into pET22b(+) and expressed in Escherichia coli BL21. Purification of recombinant β-1,3-1,4-glucanase was conducted by affinity chromatography using a Ni-NTA column. Enzyme specificity of β-1,3-1,4-glucanase was confirmed based on substrate specificity. The optimal temperature and pH of the purified enzyme towards barley β-glucan were 50 °C and pH 6, respectively. More than 80 % of activity was retained at temperatures of 30-70 °C and pH values of 4-9, which differed from all other bacterial β-1,3-1,4-glucanases. The degradation products of barley β-glucan by β-1,3-1,4-glucanase were analysed using thin-layer chromatography, and ultimately glucose was produced by treatment with cellobiase.
journal_name
Bioprocess Biosyst Engjournal_title
Bioprocess and biosystems engineeringauthors
Kim YR,Kim EY,Lee JM,Kim JK,Kong ISdoi
10.1007/s00449-013-0896-4subject
Has Abstractpub_date
2013-06-01 00:00:00pages
721-7issue
6eissn
1615-7591issn
1615-7605journal_volume
36pub_type
杂志文章abstract::An optimized repeated-fed-batch fermentation process for the synthesis of dihydroxyacetone (DHA) from glycerol utilizing Gluconobacter oxydans is presented. Cleaning, sterilization, and inoculation procedures could be reduced significantly compared to the conventional fed-batch process. A stringent requirement was tha...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-003-0338-9
更新日期:2003-12-01 00:00:00
abstract::In this study, a new-generation subsurface upflow wetland (SUW) system packed with the unique sorption media was introduced for nutrient removal. To explore the interface between hydraulic and environmental performance, a tracer study was carried out in concert with a transport model to collectively provide hydraulic ...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-011-0578-z
更新日期:2012-03-01 00:00:00
abstract::Microalgae are considered as a promising biotechnological strategy to capture CO2 from biogas, producing biomass with valuable energetic compounds. This study has evaluated the biotechnological potential to fix CO2 from biogas and biomass production by Scenedesmus obliquus cultured in four different formulations of cu...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-017-1828-5
更新日期:2017-12-01 00:00:00
abstract::Experiments carried out with the dissolved oxygen tension (DOT) maintained during fermentation at 0, 10, 50, 70 and 100% showed a direct effect of the dissolved oxygen levels on weissellin A production with no correlative increase on biomass. An estimate of the yield of weissellin A per gram biomass revealed the 50% D...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-012-0689-1
更新日期:2012-08-01 00:00:00
abstract::Armillaria sp. F022, a white-rot fungus isolated from decayed wood in tropical rain forest was used to biodegrade anthracene in cultured medium. The percentage of anthracene removal by Armillaria sp. F022 reached 13 % after 7 days and at the end of the experiment, anthracene removal level was at 87 %. The anthracene r...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-012-0850-x
更新日期:2013-09-01 00:00:00
abstract::The major nutrients, pH and temperature were evaluated for the exopolysaccharide (EPS) production by Daedalea dickinsii in submerged culture to derive an optimal medium composition and conditions as follows: 50 g/L maltose, 5 g/L soy peptone, 5 mM CaCl(2), at pH 6.0 and 28 °C. A purified EPS fraction was attained from...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-013-1111-3
更新日期:2014-07-01 00:00:00
abstract::Cheese whey fermentation to ethanol using immobilized Kluyveromyces marxianus cells was investigated in batch and continuous operation. In batch fermentation, the yeast cells were immobilized in carboxymethyl cellulose (CMC) polymer and also synthesized graft copolymer of CMC with N-vinyl-2-pyrrolidone, denoted as CMC...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-016-1610-0
更新日期:2016-09-01 00:00:00
abstract::The effects of light, pH and organic carbon sources were investigated on hydrogen production by algae. An optical fiber was examined as an internal light source. The optical fiber rendered prolonged lag time and total time of hydrogen production. The optimal pH to produce hydrogen for Chlorella sp. was 8.0. Glucose, f...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-012-0819-9
更新日期:2013-07-01 00:00:00
abstract::The effluent from the biogas process was tested as a nutrient source during cultivation of the protein-rich and edible microalgae Spirulina (Arthrospira platensis) and compared with conventional Spirulina medium. Equal biomass production was observed until late exponential phase and no significant differences could be...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-016-1726-2
更新日期:2017-04-01 00:00:00
abstract::To examine the effects of ammonium salts on tetramethylpyrazine (TTMP) production by Bacillus subtilis CCTCC M 208157, different ammonium salts were tested, and diammonium phosphate (DAP) was found to have a predominant effect on stimulating TTMP synthesis. The DAP requirements for TTMP production were then investigat...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-010-0419-5
更新日期:2010-10-01 00:00:00
abstract::Erythromycin A is an important antibiotic. A chemically defined synthetic medium for erythromycin production was systematically optimized in this study. A high-throughput method was employed to reduce the number of components and optimize the concentration of each component. After two round single composition deletion...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-018-1980-6
更新日期:2018-10-01 00:00:00
abstract::Organic solid waste composting is a complex process that involves many coupled physical, chemical and biological mechanisms. To understand this complexity and to ease in planning, design and management of the composting plant, mathematical model for simulation is usually applied. The aim of this paper is to develop a ...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-015-1492-6
更新日期:2016-01-01 00:00:00
abstract::The characterization of anodic microbial communities is of great importance in the study of microbial fuel cells (MFCs). These kinds of devices mainly require a high abundance of anode respiring bacteria (ARB) in the anode chamber for optimal performance. This study evaluated the effect of different enrichments of env...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-019-02074-0
更新日期:2019-05-01 00:00:00
abstract::This work studies the sequential execution of dilute sulfuric acid (DAP) (0.1-0.75 %, v/v) and dilute sodium hydroxide (AKP) (0.25-3 %, w/v) [i.e., DAP followed by AKP (DAP+AKP) and vice versa (AKP+DAP)] at low temperatures (<121 °C) and short reaction times (5-60 min) for maximizing sugar recovery in the enzymatic hy...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-015-1355-1
更新日期:2015-06-01 00:00:00
abstract::Twenty-nine actinobacterial strains were isolated from marine sponge Spongia officinalis and screened for antagonistic activity against various bacterial and fungal pathogens. The active antibiotic producer MAPS15 was identified as Streptomyces sp. using 16S rRNA phylogenetic analysis. The critical control factors wer...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-013-1023-2
更新日期:2014-03-01 00:00:00
abstract::Chemical composition and physical structure of solid substrate have significantly impacts on fermentation performance. The aqueous ammonia was used to pretreat rice straw. Furthermore, the feasibility of pretreatment to improve laccase production was also evaluated in terms of the enzymatic digestibility, chemical str...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-018-02060-y
更新日期:2019-04-01 00:00:00
abstract::Phosphatidylserine is widely used in food, health, chemical and pharmaceutical industries. The phospholipase D-mediated green synthesis of phosphatidylserine has attracted substantial attention in recent years. In this study, the phospholipase D was heterologously expressed in Bacillus subtilis, Pichia pastoris, and C...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-019-02116-7
更新日期:2019-07-01 00:00:00
abstract::Antrodia cinnamomea has recently become a well-known medicinal mushroom in Taiwan. Bioactive compounds found in A. cinnamomea include: polysaccharide, sesquiterpene lactone, steroids and triterpenoids. The aim of this study was to evaluate the feasibility of adding citrus peel extract to enhance the formation of bioac...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-012-0712-6
更新日期:2012-10-01 00:00:00
abstract::Many bacteria reduce inorganic sulfate to sulfide to satisfy their need for sulfur, one of the most important elements for biological life. But little is known about the metabolic pathways involving hydrogen sulfide (H₂S) in mesophilic bacteria. By genomic sequence analysis, a complete set of genes for the assimilator...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-012-0839-5
更新日期:2013-10-01 00:00:00
abstract::A two-phase dispersion technique, termed emulsification-internal gelation, is proposed for encapsulation of activated sludge in gellan gum microbeads. The influence of emulsion parameters on size distribution of microbeads was investigated. Mean diameter of microbeads varied within a range of 34-265 microm as a descen...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-004-0360-6
更新日期:2004-07-01 00:00:00
abstract::Cucurbitacin B (CuB) and its glycoside, cucurbitacin B 2-o-β-D-glucoside (CuBg), abundantly occur in the pedicels of Cucumis melo. Compared with CuB, CuBg is not efficiently extracted from the pedicels. Furthermore, the anticancer activity of CuBg is lower than that of the aglycone. A process for CuBg biotransformatio...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-016-1621-x
更新日期:2016-09-01 00:00:00
abstract::A serine protease-producing marine bacterial strain named as PT-1 was isolated and identified as a family of Marinomonas arctica, based on molecular characterization of 16S rRNA gene sequence, phylogenetic tree, and fatty acid composition analyses. Optimized culture conditions for growth of the bacterium PT-1 and prod...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-015-1514-4
更新日期:2016-02-01 00:00:00
abstract::Understanding process parameter interactions and their effects on mammalian cell cultivations is an essential requirement for robust process scale-up. Furthermore, knowledge of the relationship between the process parameters and the product critical quality attributes (CQAs) is necessary to satisfy quality by design g...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-016-1693-7
更新日期:2017-02-01 00:00:00
abstract::L-Lysine is an essential amino acid that can be produced by chemical processes from fossil raw materials, as well as by microbial fermentation, the latter being a more efficient and environmentally friendly procedure. In this work, the production process of L-lysine-HCl is studied using a systematic approach based on ...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-017-1766-2
更新日期:2017-07-01 00:00:00
abstract::We propose an adaptive control law that allows one to identify unstable steady states of the open-loop system in the single-species chemostat model without the knowledge of the growth function. We then show how one can use this control law to trace out (reconstruct) the whole graph of the growth function. The process ...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-013-0912-8
更新日期:2013-10-01 00:00:00
abstract::Novel strategies to facilitate the transition from batch to continuous simultaneous saccharification and fermentation were studied in this work. Implementing these strategies in bioethanol production plants to change production to a continuous mode will avoid large modifications in the process configuration. Therefore...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-013-0969-4
更新日期:2013-12-01 00:00:00
abstract::Changes in the microbial community of an enhanced biological phosphorus removal (EBPR) activated sludge system under different influent phosphorus/carbon (P/C) ratio conditions were investigated through evaluation of population respiratory quinone profiles. A total of 13 types of respiratory quinone homologs consistin...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-006-0065-0
更新日期:2006-08-01 00:00:00
abstract::Silver nanoparticles (Ag NPs) are being increasingly used in many consumer products owing to their excellent antimicrobial properties. The continuous use of Ag NPs in consumer products will lead to environmental release. The present study evaluated the toxic effects and the possible underlying mechanism of Ag NPs on P...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-015-1365-z
更新日期:2015-07-01 00:00:00
abstract::Freshwater sources are limited and access to clean water is an acute challenge in recent decades. The sustainable water treatments methods are need of time and water desalination is one of the most interesting technology. Most desalination technologies are required high energy input while Microbial Desalination Cells ...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-020-02433-2
更新日期:2021-01-01 00:00:00
abstract::Safe and eco-friendly alternatives to currently used hazardous chemico-physical methods of silver nanoparticles (AgNPs) synthesis are need of time. Rapid, low cost, selective detection of toxic metals in environmental sample is important to take safety action. Toxicity assessment of engineered AgNPs is essential to av...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-014-1200-y
更新日期:2014-11-01 00:00:00