Abstract:
:Cheese whey fermentation to ethanol using immobilized Kluyveromyces marxianus cells was investigated in batch and continuous operation. In batch fermentation, the yeast cells were immobilized in carboxymethyl cellulose (CMC) polymer and also synthesized graft copolymer of CMC with N-vinyl-2-pyrrolidone, denoted as CMC-g-PVP, and the efficiency of the two developed cell entrapped beads for lactose fermentation to ethanol was examined. The yeast cells immobilized in CMC-g-PVP performed slightly better than CMC with ethanol production yields of 0.52 and 0.49 g ethanol/g lactose, respectively. The effect of supplementation of cheese whey with lactose (42, 70, 100 and 150 g/l) on fermentative performance of K. marxianus immobilized in CMC beads was considered and the results were used for kinetic studies. The first order reaction model was suitable to describe the kinetics of substrate utilization and modified Gompertz model was quite successful to predict the ethanol production. For continuous ethanol fermentation, a packed-bed immobilized cell reactor (ICR) was operated at several hydraulic retention times; HRTs of 11, 15 and 30 h. At the HRT of 30 h, the ethanol production yield using CMC beads was 0.49 g/g which implies that 91.07 % of the theoretical yield was achieved.
journal_name
Bioprocess Biosyst Engjournal_title
Bioprocess and biosystems engineeringauthors
Roohina F,Mohammadi M,Najafpour GDdoi
10.1007/s00449-016-1610-0subject
Has Abstractpub_date
2016-09-01 00:00:00pages
1341-9issue
9eissn
1615-7591issn
1615-7605pii
10.1007/s00449-016-1610-0journal_volume
39pub_type
杂志文章abstract::L-aspartate ammonia-lyase from Bacillus sp. YM55-1 (AspB, EC 4.3.1.1) catalyzes the reversible conversion of L-aspartate (Asp) into fumarate and ammonia with a high specific activity toward the substrate. AspB was expressed in Escherichia coli and partially purified by heat precipitation and saturation with ammonium s...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-012-0732-2
更新日期:2012-10-01 00:00:00
abstract::The paper describes a recombinant Schneider 2 (rS2) cell culture and protein expression in a bioreactor. S2 cells were transfected with a plasmid containing a fusion protein (human mu opioid receptor, hMOR, and green fluorescent protein, EGFP) under the control of inducible metallothionein promoter. A bioprocess in a ...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-005-0033-0
更新日期:2006-04-01 00:00:00
abstract::High amounts of outer membrane (OM) components were released in glucose-limited fed-batch (GLFB) cultures at 37 degrees C at specific growth rates approaching 0.05 h(-1). Endotoxin analyses from a 20 degrees C GLFB culture gave similar results. An alternative fermentation technique, the temperature-limited fed-batch (...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-004-0377-x
更新日期:2005-04-01 00:00:00
abstract::Biodiesel production from microalgae depends on the biomass concentration and lipid content in microalgal cells. Photobioreactors (PBRs) facilitates cultivation of microalgae and renders better process control than open systems. However, reactor configuration and consequential hydrodynamics considerably influence biom...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-020-02342-4
更新日期:2020-08-01 00:00:00
abstract::Industrial grade soluble corn starch was used directly and effectively as the fermentation substrate for microbial exopolysaccharides production. Bacillus subtilis mutant strain NJ308 grew with untreated starch raw material as the sole carbon source. The real-time PCR results demonstrated that up-regulated genes encod...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-018-1915-2
更新日期:2018-06-01 00:00:00
abstract::Vitamin B12 is a crucial fine chemical that is widely used in the pharmaceutical, food and chemical industries, and its production solely dependents on microbial fermentation. We previously constructed an artificial vitamin B12 biosynthesis pathway in Escherichia coli, but the yield of the engineered strains was low. ...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-020-02355-z
更新日期:2020-10-01 00:00:00
abstract::Recycled paper mills produce large quantities of fibrous rejects and fines which are usually sent to landfills as solid waste. These cellulosic materials can be enzymatically hydrolyzed into sugars for the production of biofuels and biomaterials. Paper mill wastes also contain large amounts of calcium carbonate which ...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-017-1745-7
更新日期:2017-06-01 00:00:00
abstract::Tissue engineered constructs reported to date have been prepared primarily from poly(glycolic) acid or collagen scaffolds onto which cells are grown and matured. In this paper we report experimental data to demonstrate the use of a natural, human protein, as a tubular scaffold for vascular grafting. Using a manual and...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s004490100268
更新日期:2002-04-01 00:00:00
abstract::The development of a safe and eco-friendly method for metal nanoparticle synthesis has an increasing demand, due to emerging environmental and biological harms of hazardous chemicals used in existing nanosynthesis methods. The present investigation reports a rapid one-step, eco-friendly and green approach for the form...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-018-1905-4
更新日期:2018-05-01 00:00:00
abstract::Antrodia cinnamomea has recently become a well-known medicinal mushroom in Taiwan. Bioactive compounds found in A. cinnamomea include: polysaccharide, sesquiterpene lactone, steroids and triterpenoids. The aim of this study was to evaluate the feasibility of adding citrus peel extract to enhance the formation of bioac...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-012-0712-6
更新日期:2012-10-01 00:00:00
abstract::This work aimed to develop an optimal carbon source feeding strategy to achieve maximal production of heparosan as a precursor of bioengineered heparin by Escherichia coli K5. Glycerol gave higher heparosan titer and productivity compared to glucose. The maximum heparosan production (187 mg/L) and heparosan productivi...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-012-0708-2
更新日期:2012-09-01 00:00:00
abstract::L-arabinose isomerase (L-AI) (EC 5. 3. 1. 4. L-AI) that mediates the isomerization of D-galactose to D-tagatose was isolated from Lactobacillus brevis (MF 465792), and was further purified and characterized. Pure enzyme with molecular weight of 60.1 kDa was successfully obtained after the purification using Native-PAG...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-018-2018-9
更新日期:2019-01-01 00:00:00
abstract::In this study, the silica nanoparticles surface was modified by carboxylic acid with different number of alkyl chain and two different lipases were immobilized on it to increase the glycerolysis activity of the immobilized lipases in organic solvent system. Silica nanoparticles of about 15 nm diameter were grafted wit...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-017-1852-5
更新日期:2018-01-01 00:00:00
abstract::The hydrolase (Thermobifida fusca hydrolase; TfH) from T. fusca was produced in Escherichia coli as fusion protein using the OmpA leader sequence and a His(6) tag. Productivity could be raised more than 100-fold. Both batch and fed-batch cultivations yield comparable cell specific productivities whereas volumetric pro...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-006-0069-9
更新日期:2006-08-01 00:00:00
abstract::Betaine can act as a stress protectant, methyl donor, or enzyme stabilizer in vitro for the biosynthesis of structurally complex compounds. The performances of betaine type and concentration on the metabolic processes of Escherichia coli JLTHR in a 5-L fermentor were investigated. The results showed that the maximum L...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-018-1978-0
更新日期:2018-10-01 00:00:00
abstract::Experiments carried out with the dissolved oxygen tension (DOT) maintained during fermentation at 0, 10, 50, 70 and 100% showed a direct effect of the dissolved oxygen levels on weissellin A production with no correlative increase on biomass. An estimate of the yield of weissellin A per gram biomass revealed the 50% D...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-012-0689-1
更新日期:2012-08-01 00:00:00
abstract::Lantana camara, an abundantly available non-edible lignocellulosic biomass has been found to be a potential feedstock for ethanol production. The substrate was first pretreated with laccase followed by simultaneous saccharification and fermentation using cellulase and Saccharomyces cerevisiae, respectively. Laccase wa...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-014-1172-y
更新日期:2014-10-01 00:00:00
abstract::The major nutrients, pH and temperature were evaluated for the exopolysaccharide (EPS) production by Daedalea dickinsii in submerged culture to derive an optimal medium composition and conditions as follows: 50 g/L maltose, 5 g/L soy peptone, 5 mM CaCl(2), at pH 6.0 and 28 °C. A purified EPS fraction was attained from...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-013-1111-3
更新日期:2014-07-01 00:00:00
abstract::In this study, serine protease (subtilisin Carlsberg) was immobilized on pentynyl dextran (PyD, O-alkynyl ether of dextran, 1) and used for the transesterification of N-acetyl-L-phenylalanine ethyl ester (2) with different aliphatic (1-propanol, 1-butanol, 1-pentanol, 1-hexanol) and aromatic (benzyl alcohol, 2-phenyl ...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-013-1038-8
更新日期:2014-04-01 00:00:00
abstract::Membrane bioreactors (MBR) are being increasingly used for wastewater treatment. Mathematical modeling of MBR systems plays a key role in order to better explain their characteristics. Several MBR models have been presented in the literature focusing on different aspects: biological models, models which include solubl...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-012-0806-1
更新日期:2013-04-01 00:00:00
abstract::Economical production of xylanase and three cellulases, endo-β-1,4-glucanase (CMCase), exo-β-1,4-glucanase (FPase), β-glucosidase (BGL) was studied in submerged fermentation using cane molasses medium. A statistical optimization approach involving Plackett-Burman design and response surface methodology (RSM) resulted ...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-015-1502-8
更新日期:2016-01-01 00:00:00
abstract::Amano lipase AK from P. fluorescens was immobilized on different types of chitosan-containing supports. Chitosan lower molecular weight (2.5%), chitosan lower molecular weight/sodium alginate (2.5%/2.5%) and chitosan lower molecular weight/carrageenan (2.5%/2.5%) allowed the highest values of immobilization yields (IY...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-020-02487-2
更新日期:2021-01-03 00:00:00
abstract::Lipase-catalyzed caffeic acid phenethyl ester (CAPE) synthesis in ionic liquid, 1-ethyl-3-methylimidazolium bis[(trifluoromethyl)sulfonyl]imide ([Emim][Tf(2)N]), was investigated in this study. The effects of several reaction conditions, including reaction time, reaction temperature, substrate molar ratio of phenethyl...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-013-0906-6
更新日期:2013-06-01 00:00:00
abstract::Sorghum stalks were evaluated to generate clean sugars; enzymatic hydrolysis efficiency of acid pretreated biomass was significantly low (12.3 g sugar/g pretreated biomass) compared to alkali method (49.1 g sugar/g pretreated biomass). Inhibitory compounds derived from acid pretreatment, such as hydroxymethyl furfural...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章,评审
doi:10.1007/s00449-016-1727-1
更新日期:2017-04-01 00:00:00
abstract::Sodium dodecyl sulfate (SDS) is a widely used anionic surfactant, which finds its way to the receiving water body due to the incapability of conventional wastewater treatment systems to completely remove it. A hybrid treatment system consisting of upflow microbial fuel cell (MFC) with titanium dioxide (TiO2) as a phot...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-020-02396-4
更新日期:2020-11-01 00:00:00
abstract::A kinetic model for glucose and xylose co-substrate uptake in Saccharomyces cerevisiae is presented. The model couples the enzyme kinetics with the glucose-dependent genetic expression of the individual transport proteins. This novel approach implies several options for optimizing the co-substrate utilization. Interes...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-007-0169-1
更新日期:2008-06-01 00:00:00
abstract::Laccases are versatile oxidases that are capable of decolorizing various synthetic dyes. Recombinant Bacillus amyloliquefaciens laccase was immobilized as magnetic cross-linked enzyme aggregates (M-CLEAs) for application in dye decolorization. Several parameters influencing the activity recovery were evaluated during ...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-020-02481-8
更新日期:2020-11-27 00:00:00
abstract::To improve the performance of covalently immobilized penicillin acylase (PA), the immobilization was carried out in mesocellular silica foams (MCFs) using p-benzoquinone as cross linker. The characterizations of the immobilized enzyme were studied carefully. The results showed that the relative activity of the immobil...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-007-0189-x
更新日期:2008-08-01 00:00:00
abstract::Organic solid waste composting is a complex process that involves many coupled physical, chemical and biological mechanisms. To understand this complexity and to ease in planning, design and management of the composting plant, mathematical model for simulation is usually applied. The aim of this paper is to develop a ...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-015-1492-6
更新日期:2016-01-01 00:00:00
abstract::The fungicide carbendazim is an ecotoxic agent affecting aquatic biota. Due to its suspected hormone-disrupting effects, it is considered a "priority hazard substance" by the Water Framework Directive of the European Commission, and its degradation is of major concern. In this work, a horizontal tubular biofilm reacto...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-016-1717-3
更新日期:2017-04-01 00:00:00