Effects of betaine supplementation on L-threonine fed-batch fermentation by Escherichia coli.

Abstract:

:Betaine can act as a stress protectant, methyl donor, or enzyme stabilizer in vitro for the biosynthesis of structurally complex compounds. The performances of betaine type and concentration on the metabolic processes of Escherichia coli JLTHR in a 5-L fermentor were investigated. The results showed that the maximum L-threonine production of 127.3 g/L and glucose conversion percentage of 58.12% was obtained fed with the glucose solution containing 2 g/L betaine hydrochloride, which increased by 14.5 and 6.87% more compared to that of the control, respectively. This study presents an analysis of the metabolic fluxes of E. coli JLTHR for the production of L-threonine with betaine supplementation. When betaine was fed into the fermentation culture medium, the metabolic flux entering into the pentose phosphate pathway (HMP) and biosynthesis route of L-threonine increased by 57.3 and 10.1%, respectively. In conclusion, exogenous addition of betaine was validated to be a feasible and efficacious approach to improve L-threonine production.

journal_name

Bioprocess Biosyst Eng

authors

Su Y,Guo QQ,Wang S,Zhang X,Wang J

doi

10.1007/s00449-018-1978-0

subject

Has Abstract

pub_date

2018-10-01 00:00:00

pages

1509-1518

issue

10

eissn

1615-7591

issn

1615-7605

pii

10.1007/s00449-018-1978-0

journal_volume

41

pub_type

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