Abstract：
:Economical production of xylanase and three cellulases, endo-β-1,4-glucanase (CMCase), exo-β-1,4-glucanase (FPase), β-glucosidase (BGL) was studied in submerged fermentation using cane molasses medium. A statistical optimization approach involving Plackett-Burman design and response surface methodology (RSM) resulted in the production of 72,410, 36,420, 32,420 and 5180 U/l of xylanase, CMCase, FPase and β-glucosidase, respectively. Optimization resulted in more than fourfold improvements in production of xylanolytic and cellulolytic enzymes. Scale up of enzymes production in shake flasks of varied volumes was sustainable, suggesting a good scope for large scale enzyme production. Addition of microparticles engineered fungal morphology and enhanced enzymes production. Xylanase of S. thermophile is a neutral xylanase displaying its optimal activity at 60 °C while all the cellulases are optimally active at pH 5.0 and 60 °C. The efficacy of enzyme cocktail in waste tea cup paper and rice straw hydrolysis showed that maximum sugar yield of 578.12 and 421.79 mg/g substrate for waste tea cup and rice straw, respectively, were achieved after 24 h. Therefore, concomitant production of cellulolytic and xylanolytic enzymes will be beneficial for the saccharification of lignocellulosics in generating both monomeric and oligomeric sugars for biofuels and other biotechnological applications.
journal_name
Bioprocess Biosyst Engjournal_title
Bioprocess and biosystems engineeringauthors
Bala A,Singh Bdoi
10.1007/s00449-015-1502-8subject
Has Abstractpub_date
2016-01-01 00:00:00pages
181-91issue
1eissn
1615-7591issn
1615-7605pii
10.1007/s00449-015-1502-8journal_volume
39pub_type
杂志文章abstract：:The hydrolase (Thermobifida fusca hydrolase; TfH) from T. fusca was produced in Escherichia coli as fusion protein using the OmpA leader sequence and a His(6) tag. Productivity could be raised more than 100-fold. Both batch and fed-batch cultivations yield comparable cell specific productivities whereas volumetric pro...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-006-0069-9
更新日期：2006-08-01 00:00:00
abstract：:Of various metal ions (Ca2+, Cr3+, Cu2+, Fe2+, Mg2+, Mn2+, Ni2+ and Zn2+) added to the culture medium of Agrobacterium tumefaciens at 1 mM, only Ca2+ increased Coenzyme Q10 (CoQ(10)) content in cells without the inhibition of cell growth. In a pH-stat fed-batch culture, supplementation with 40 mM of CaCO3 increased th...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-009-0318-9
更新日期：2009-08-01 00:00:00
abstract：:An environmentally friendly chemical, tetrakis(hydroxymethyl)phosphonium sulfate (THPS), was used as a metabolic uncoupler to reduce sludge production in a pilot-scale anaerobic/anoxic/oxic process. The results show that the addition of THPS (1.08-1.86 mL/m(3) influent) in the sludge return section could reduce waste ...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-013-1022-3
更新日期：2014-03-01 00:00:00
abstract：:Microbial lipid produced using yeast fermentation with inexpensive carbon sources such as lignocellulosic hydrolyzate can be an alternative feedstock for biodiesel production. Several inhibitors that can be generated during acid hydrolysis of lignocellulose were added solely or together into the culture medium to stud...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-012-0684-6
更新日期：2012-08-01 00:00:00
abstract：:A novel bacterial isolate, capable of producing extracellular highly thermostable, halo-alkali-stable and cellulase-free xylanase, was isolated from soil and identified as Bacillus halodurans TSPV1 by polyphasic approach. The Plackett-Burman design identified wheat bran, lactose, tryptone and NaCl as the factors that ...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-012-0811-4
更新日期：2013-05-01 00:00:00
abstract：:In this study, sodium gluconate was applied as a novel carbon source for the fuel ethanol production using an engineered Escherichia coli strain KO11 in batch fermentations. Ethanol and acetic acid were produced as two major products as well as small amount of lactic acid during the fermentation. Compared to the conve...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-013-0938-y
更新日期：2013-11-01 00:00:00
abstract：:A new online monitoring technique to measure the physiological parameters, dissolved oxygen (DO) and pH of microbial cultures in continuously shaken 24-well microtiter plates (MTP) is introduced. The new technology is based on immobilised fluorophores at the bottom of standard 24-well MTPs. The sensor MTP is installed...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-005-0010-7
更新日期：2005-11-01 00:00:00
abstract：:Novel strategies to facilitate the transition from batch to continuous simultaneous saccharification and fermentation were studied in this work. Implementing these strategies in bioethanol production plants to change production to a continuous mode will avoid large modifications in the process configuration. Therefore...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-013-0969-4
更新日期：2013-12-01 00:00:00
abstract：:The use of fed-batch extractive fermentation can overcome inhibitory effects caused by the substrate and ethanol to the yeast cells, since it allows regulate the substrate concentration and remove the product as it is produced. The present study describes the modelling and experimental validation of ethanol production...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-020-02414-5
更新日期：2020-12-01 00:00:00
abstract：:Microalgal carotenoids are attractive health ingredients, but their production should be optimized to improve cost-effectiveness. Understanding cellular physiology centered on carotenoid synthesis is the prerequisite for this work. Therefore, systematic correlation analyses were conducted among chlorophyll, carotenoid...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-018-1957-5
更新日期：2018-09-01 00:00:00
abstract：:The treatment of acidic (pH 6.5-3), sulfate- (2-3 g/L), Zn- and Cu- (total metal 0-500 mg/L) containing wastewater was studied in a four-stage anaerobic baffled reactor (ABR) at 35 °C for 250 days. Ethanol was supplemented (COD/SO4(2-) = 0.67) as carbon and electron source for sulfate reducing bacteria. Sulfate reduct...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-010-0423-9
更新日期：2010-10-01 00:00:00
abstract：:The effect of different process variables of reverse micelle extraction process like pH, addition of surfactant (AOT) concentration and potassium chloride (KCl) concentration on amylase recovery has been studied and analysed. Solid-state fermentation was used for the production of amylase enzyme. Response surface meth...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-007-0172-6
更新日期：2008-06-01 00:00:00
abstract：:Docosahexaenoic acid (DHA) percentage in total fatty acids (TFAs) is an important index in DHA microbial production. In this study, the change of DHA percentage in response to fermentation stages and the strategies to increase DHA percentage were investigated. Two kinds of conventional nitrogen sources, monosodium glu...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-013-1057-5
更新日期：2014-05-01 00:00:00
abstract：:Unfolded protein response (UPR) usually happens when expressing heterologous proteins in high level, which may help cells to facilitate protein processing. Here, we evaluated the effects of the UPR activator HAC1p on a raw-starch hydrolyzing α-amylase (Gs4j-amyA), so as to improve heterologous production of the enzyme...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-016-1701-y
更新日期：2017-03-01 00:00:00
abstract：:Maltose degrading enzyme was immobilized within agar-agar support via entrapment method due to its industrial utilization. The maximum immobilization efficiency (82.77%) was achieved using 4.0% agar-agar keeping the diameter of bead up to 3.0 mm. The matrix entrapment showed maximum catalytic activity at pH 7.0 and te...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-014-1302-6
更新日期：2015-04-01 00:00:00
abstract：:This study evaluated an anaerobic fluidized bed reactor to produce hydrogen from sugarcane molasses of 25 g-COD L-1. The reactor of 1.2 L working volume contained shredded tires as support material. The inoculum was sludge obtained in a UASB reactor of a sewage treatment plant. The AFBR was operated at hydraulic reten...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-020-02443-0
更新日期：2020-09-26 00:00:00
abstract：:In response to sudden decrease in osmotic pressure, halophilic microorganisms secrete their accumulated osmolytes. This specific stress response, combined with physiochemical responses to the altered environment, influence the membrane properties and integrity of cells, with consequent effects on growth and yields in ...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-013-0957-8
更新日期：2013-12-01 00:00:00
abstract：:Recycled paper mills produce large quantities of fibrous rejects and fines which are usually sent to landfills as solid waste. These cellulosic materials can be enzymatically hydrolyzed into sugars for the production of biofuels and biomaterials. Paper mill wastes also contain large amounts of calcium carbonate which ...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-017-1745-7
更新日期：2017-06-01 00:00:00
abstract：:A novel and efficient facultative anaerobic denitrifying bacterium was isolated and identified as Pseudomonas citronellolis WXP-4. The strain WXP-4 could achieve 100% nitrate and nitrite removal efficiency utilizing sodium succinate as a carbon source, C/N ratio 7, pH 7.0, and temperature 40 °C under both aerobic and ...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-019-02276-6
更新日期：2020-05-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-020-02327-3
更新日期：2020-07-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-011-0523-1
更新日期：2011-08-01 00:00:00
abstract：:This paper demonstrates Pseudomonas cepacia lipase catalyzed hydrolysis of p-nitrophenyl palmitate under irradiation of light with wavelengths of 250-750 nm. The reaction follows Michaelis-Menten Kinetics and the light irradiation increases the overall rate of hydrolysis. Using Lineweaver-Burk plot K M and V max value...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-014-1213-6
更新日期：2014-11-01 00:00:00
abstract：:Estimation of the energy capacity of a microbial cell mass on the basis of its lipid content and elemental composition can be used for the comparative evaluation of different microbial sources of biodiesel. Lipomyces starkeyi cell mass concentration reached 94.6 g/L with 37.4% of lipids in a fed-batch process using xy...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-015-1394-7
更新日期：2015-08-01 00:00:00
abstract：:Wild-type Corynebacterium glutamicum was metabolically engineered to convert glucose and mannose into guanosine 5'-diphosphate (GDP)-L-fucose, a precursor of fucosyl-oligosaccharides, which are involved in various biological and pathological functions. This was done by introducing the gmd and wcaG genes of Escherichia...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-013-0900-z
更新日期：2013-06-01 00:00:00
abstract：:In the present study, it was presented a strategy to maximize the cutinase production by solid-state fermentation from different microorganisms and substrates. The best results were observed using Fusarium verticillioides, rice bran being the main substrate. Maximum yield of cutinase obtained by the strain was 16.22 U...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-019-02086-w
更新日期：2019-05-01 00:00:00
abstract：:Bacterial cellulose (BC) exhibits unique properties such as high purity compared to plant-based cellulose; however, commercial production of BC has remained a challenge, primarily due to the strain properties of cellulose-producing bacteria. Herein, we developed a functional and stable BC production system in genetica...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-017-1864-1
更新日期：2018-02-01 00:00:00
abstract：:4''-O-isovalerylspiramycins are the major components of bitespiramycin complex consisting of a group of 4''-O-acylated spiramycins. The availability of isovaleryl group, usually in vivo derived from leucine, one of the branched-chain amino acids, affects the content of isovaleryispiramycin significantly. In this study...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-009-0320-2
更新日期：2010-02-01 00:00:00
abstract：:Lignin-modifying enzymes have various promising applications such as biobleaching, biopulping, the functionalization of lignocellulosic materials, the modification of wood fibers, the remediation of contaminated soil and effluents, as well as improvement of the enzymatic hydrolysis of lignocellulosic substrates. In th...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-014-1243-0
更新日期：2015-01-01 00:00:00
abstract：:This work proposes a committee of Cascade Correlation neural networks as an online smoother for random measurement noise. The goals of this paper are twofold: first it intends to explore the possibilities of using a constructive neural network algorithm to learn how to filter typical noisy data from a bioreactor, CO(2...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-007-0152-x
更新日期：2008-02-01 00:00:00
abstract：:Salmonella is a major pathogen that causes acute foodborne outbreaks worldwide. Seafood, particularly shellfish, is a proven source of Salmonella spp. infection because many people prefer to eat it raw or lightly cooked. However, traditional identification methods are too time-consuming and complex to detect contamina...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-018-1895-2
更新日期：2018-05-01 00:00:00
