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Effect of visible light on catalytic hydrolysis of p-nitrophenyl palmitate by the Pseudomonas cepacia lipase immobilized on sol-gel support.

Abstract：

:This paper demonstrates Pseudomonas cepacia lipase catalyzed hydrolysis of p-nitrophenyl palmitate under irradiation of light with wavelengths of 250-750 nm. The reaction follows Michaelis-Menten Kinetics and the light irradiation increases the overall rate of hydrolysis. Using Lineweaver-Burk plot K M and V max values for the reaction in presence of light are found to be 39.07 and 66.67 mM/min/g, respectively; while for the same reaction under dark condition, the values are 7.08 and 10.21 mM/min/g. The linear form of enzyme dependent rate of reaction confirms that no mass-transfer limitations are present and the reaction is a kinetically controlled enzymatic reaction.

journal_name

Bioprocess Biosyst Eng

journal_title

Bioprocess and biosystems engineering

authors

Ganasen P,Khan MR,Kalam MA,Mahmud MS

doi

10.1007/s00449-014-1213-6

subject

Has Abstract

pub_date

2014-11-01 00:00:00

pages

2353-9

issue

11

eissn

1615-7591

issn

1615-7605

journal_volume

37

pub_type

杂志文章

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