Product formation kinetics in genetically modified E. coli bacteria: inclusion body formation.

abstract：:Long-term and excessive use of monocrotophos (MPs) pesticide leads to an accumulation of MPs residues in agricultural products. Electrochemical biosensor technology was developed as a simple and efficient method for detecting MPs. However, commercial acetylcholinesterase (AChE) sensors are not applied in practical MPs...

journal_title：Bioprocess and biosystems engineering

authors： Jiaojiao X,Bin Z,Pengyun W,Qing L,Xin S,Rui J

更新日期：2020-02-01 00:00:00

abstract：:D-Amino acid oxidase is an important biocatalyst used in a variety of fields, and its economically justified level recombinant expression in Escherichia coli has not been established. To accomplish this, after a single Phe54Tyr substitution, fusion proteins of D-amino acid oxidase from Trigonopsis variabilis (TvDAO) w...

journal_title：Bioprocess and biosystems engineering

authors： Deng S,Su E,Ma X,Yang S,Wei D

更新日期：2014-08-01 00:00:00

abstract：:Gas sparging performances of a flat sheet and tubular polymeric membranes were investigated in 3.1 m bubble column bioreactor operated in a semi batch mode. Air-water and air-CMC (Carboxymethyl cellulose) solutions of 0.5, 0.75 and 1.0 % w/w were used as interacting gas-liquid mediums. CMC solutions were employed in t...

journal_title：Bioprocess and biosystems engineering

authors： Tirunehe G,Norddahl B

更新日期：2016-04-01 00:00:00

abstract：:Tissue engineered constructs reported to date have been prepared primarily from poly(glycolic) acid or collagen scaffolds onto which cells are grown and matured. In this paper we report experimental data to demonstrate the use of a natural, human protein, as a tubular scaffold for vascular grafting. Using a manual and...

journal_title：Bioprocess and biosystems engineering

authors： Harding SI,Afoke A,Brown RA,MacLeod A,Shamlou PA,Dunnill P

更新日期：2002-04-01 00:00:00

abstract：:The yield of ethanol from oil palm empty fruit bunches (EFB) was increased on exploiting maleic acid pretreatment combined with fermentation of the pretreated whole slurry. The optimized conditions for pretreatment were to expose EFB to a high temperature (190 °C) with 1 % (w/v) maleic acid for a short time duration (...

journal_title：Bioprocess and biosystems engineering

authors： Jung YH,Kim IJ,Kim HK,Kim KH

更新日期：2014-04-01 00:00:00

abstract：:Freshwater sources are limited and access to clean water is an acute challenge in recent decades. The sustainable water treatments methods are need of time and water desalination is one of the most interesting technology. Most desalination technologies are required high energy input while Microbial Desalination Cells ...

journal_title：Bioprocess and biosystems engineering

authors： Liaquat R,Mehmood T,Khoja AH,Iqbal N,Ejaz H,Mumtaz S

更新日期：2021-01-01 00:00:00

abstract：:Naturally mevalonate-resistant acetogen Clostridium sp. MT1243 produced only 425 mM acetate during syngas fermentation. Using Clostridium sp. MT1243 we engineered biocatalyst selectively producing mevalonate from synthesis gas or CO₂/H₂ blend. Acetate production and spore formation were eliminated from Clostridium sp....

journal_title：Bioprocess and biosystems engineering

authors： Kiriukhin M,Tyurin M

更新日期：2014-02-01 00:00:00

abstract：:Chemical composition and physical structure of solid substrate have significantly impacts on fermentation performance. The aqueous ammonia was used to pretreat rice straw. Furthermore, the feasibility of pretreatment to improve laccase production was also evaluated in terms of the enzymatic digestibility, chemical str...

journal_title：Bioprocess and biosystems engineering

authors： Li G,Fu Y,Dang W,Hu R,Xue H

更新日期：2019-04-01 00:00:00

abstract：:Recombinant Escherichia coli cells were applied for the recovery of electric energy from formate. Initially, the fdh gene, which encodes formate dehydrogenase (FDH) of Mycobacterium vaccae, was introduced into E. coli cells to allow efficient degradation of formate. The constructed microbial fuel cell (MFC) with E. co...

journal_title：Bioprocess and biosystems engineering

authors： Ojima Y,Kawata T,Matsuo N,Nishinoue Y,Taya M

更新日期：2014-10-01 00:00:00

abstract：:The performance of down-flow hanging sponge (DHS) system treating UASB reactor effluent at different sponge volume of 38.2, 28.7, 19.1 and 9.6% was investigated. The hydraulic retention time (HRT) and flow rate were kept constant at 2.6 h and 0.46 m(3)/day, respectively. The results obtained indicated that reducing th...

journal_title：Bioprocess and biosystems engineering

authors： Tawfik A,Ohashi A,Harada H

更新日期：2010-09-01 00:00:00

abstract：:Formulation conditions have a significant influence on the degree of freeze/thaw (FT) stress-induced protein instabilities. Adding cryoprotectants might stabilize the induced FT stress instabilities. However, a simple preservation of protein stability might be insufficient and further methods are necessary. This study...

journal_title：Bioprocess and biosystems engineering

authors： Wöll AK,Hubbuch J

更新日期：2020-07-01 00:00:00

abstract：:This study introduces an effective method to deposit polypyrrole (PPy) on graphite felt (GF) as anode to improve the start-up performance of microbial fuel cells (MFCs). The results of scanning electron microscope (SEM) and electrochemical testing reveal that polypyrrole is able to improve the electrical conductivity ...

journal_title：Bioprocess and biosystems engineering

authors： Pu KB,Lu CX,Zhang K,Zhang H,Chen QY,Wang YH

更新日期：2020-03-01 00:00:00

abstract：:We describe a new device with parallel optical measurement of dissolved oxygen (DO) and pH in up to nine shake flasks applicable in any conventional shaking incubator. Measurement ranges are 0-500% of air saturation for oxygen and 5.5-8.5 for pH. It was used to characterize growth profiles of different L-lysine produc...

journal_title：Bioprocess and biosystems engineering

authors： Schneider K,Schütz V,John GT,Heinzle E

更新日期：2010-06-01 00:00:00

abstract：:Staphylococcal enterotoxin B (SEB) is one of many toxins produced by the Gram-positive bacterium Staphylococcal aureus. While SEB is known as the causative agent of certain food poisonings it is also considered abiological select agent. Thus, rapid and accurate identification of SEB during either surveillance or in re...

journal_title：Bioprocess and biosystems engineering

authors： Chen ZG

更新日期：2008-06-01 00:00:00

abstract：:Dissolved oxygen and pH are critical factors influencing cell growth and metabolism. In our previous work, we constructed the recombinant strain Mortierella alpina CCFM698, which has the ability to produce EPA at room temperature. However, our experiments showed that the dissolved oxygen produced by the aeration and a...

journal_title：Bioprocess and biosystems engineering

authors： Huang M,Chen H,Tang X,Lu H,Zhao J,Zhang H,Chen Y,Chen W

更新日期：2020-09-01 00:00:00

abstract：:The previous works on simulated broths are continued and developed for Propionibacterium shermanii broths. The obtained results indicated the considerable increase of kLa in presence of n-dodecane as oxygen-vector and the existence of a certain value of hydrocarbon concentration that corresponds to the maximum mass tr...

journal_title：Bioprocess and biosystems engineering

authors： Galaction AI,Cascaval D,Turnea M,Folescu E

更新日期：2005-07-01 00:00:00

abstract：:Many bacteria reduce inorganic sulfate to sulfide to satisfy their need for sulfur, one of the most important elements for biological life. But little is known about the metabolic pathways involving hydrogen sulfide (H₂S) in mesophilic bacteria. By genomic sequence analysis, a complete set of genes for the assimilator...

journal_title：Bioprocess and biosystems engineering

authors： Tan T,Liu C,Liu L,Zhang K,Zou S,Hong J,Zhang M

更新日期：2013-10-01 00:00:00

abstract：:This study aimed to assess the variability in respect of titer and properties of xylanase from Trichoderma reesei SAF3 under both solid-state and submerged fermentation. SSF was initially optimized with different agro-residues and among them wheat bran was found to be the best substrate that favored maximum xylanase p...

journal_title：Bioprocess and biosystems engineering

authors： Kar S,Sona Gauri S,Das A,Jana A,Maity C,Mandal A,Das Mohapatra PK,Pati BR,Mondal KC

更新日期：2013-01-01 00:00:00

abstract：:Ginsenoside compound K (CK) with a wide range of pharmacological activities has been widely used in the healthcare product industry. However, the application of CK is limited by low productivity and difficult separation. The purpose of this study is to convert ginsenoside Rb1 into CK by improving conversion efficiency...

journal_title：Bioprocess and biosystems engineering

authors： Ma Z,Mi Y,Han X,Li H,Tian M,Duan Z,Fan D,Ma P

更新日期：2020-07-01 00:00:00

abstract：:This study evaluated an anaerobic fluidized bed reactor to produce hydrogen from sugarcane molasses of 25 g-COD L-1. The reactor of 1.2 L working volume contained shredded tires as support material. The inoculum was sludge obtained in a UASB reactor of a sewage treatment plant. The AFBR was operated at hydraulic reten...

journal_title：Bioprocess and biosystems engineering

authors： Chaves TC,Gois GNSB,Peiter FS,Vich DV,de Amorim ELC

更新日期：2020-09-26 00:00:00

abstract：:The effects of light, pH and organic carbon sources were investigated on hydrogen production by algae. An optical fiber was examined as an internal light source. The optical fiber rendered prolonged lag time and total time of hydrogen production. The optimal pH to produce hydrogen for Chlorella sp. was 8.0. Glucose, f...

journal_title：Bioprocess and biosystems engineering

authors： Rashid N,Lee K,Han JI,Gross M

更新日期：2013-07-01 00:00:00

abstract：:Lysozyme, a type of natural enzyme, has been widely used for bacteriostatic functionalization of various materials due to its efficient and selective antibacterial properties. Herein, we report the preparation and characterization of an eco-friendly antibacterial textile based on the immobilization of lysozyme from ch...

journal_title：Bioprocess and biosystems engineering

authors： Yang W,Zhang N,Wang Q,Wang P,Yu Y

更新日期：2020-09-01 00:00:00

abstract：:Enzymatic modification of starch using long chain fatty acid makes it thermoplastic suitable for a myriad of industrial applications. An industrial lipase preparation produced by Burkholderia cepacia (lipase PS) was used for modification of cassava starch with two acyl donors, lauric acid and palmitic acid. Reactions ...

journal_title：Bioprocess and biosystems engineering

authors： Rajan A,Abraham TE

更新日期：2006-06-01 00:00:00

abstract：:Vitamin B12 is a crucial fine chemical that is widely used in the pharmaceutical, food and chemical industries, and its production solely dependents on microbial fermentation. We previously constructed an artificial vitamin B12 biosynthesis pathway in Escherichia coli, but the yield of the engineered strains was low. ...

journal_title：Bioprocess and biosystems engineering

authors： Li D,Fang H,Gai Y,Zhao J,Jiang P,Wang L,Wei Q,Yu D,Zhang D

更新日期：2020-10-01 00:00:00

abstract：:Wild-type Corynebacterium glutamicum was metabolically engineered to convert glucose and mannose into guanosine 5'-diphosphate (GDP)-L-fucose, a precursor of fucosyl-oligosaccharides, which are involved in various biological and pathological functions. This was done by introducing the gmd and wcaG genes of Escherichia...

journal_title：Bioprocess and biosystems engineering

authors： Chin YW,Park JB,Park YC,Kim KH,Seo JH

更新日期：2013-06-01 00:00:00

abstract：:The lack of sensors for some relevant state variables in fermentation processes can be coped by developing appropriate software sensors. In this work, NARX-ANN, NARMAX-ANN, NARX-SVM and NARMAX-SVM models are compared when acting as software sensors of biomass concentration for a solid substrate cultivation (SSC) proce...

journal_title：Bioprocess and biosystems engineering

authors： Acuña G,Ramirez C,Curilem M

更新日期：2014-01-01 00:00:00

abstract：:The aim of this study was to investigate the efficacy of a hybrid biofilm pilot-scale treatment plant, designed with a novel configuration by the integration of a fixed-film system, to improve nitrogen removal. The pilot-scale system was established at a wastewater treatment plant in Istanbul and operated based on str...

journal_title：Bioprocess and biosystems engineering

authors： Güneş G,Hallaç E,Özgan M,Ertürk A,Okutman Taş D,Çokgor E,Güven D,Takacs I,Erdinçler A,Insel G

更新日期：2019-03-01 00:00:00

abstract：:The performance and operational stability of a pilot-scale static granular bed reactor (SGBR) for the treatment of dairy processing wastewater were investigated under a wide range of organic and hydraulic loading rates and temperature conditions. The SGBR achieved average chemical oxygen demand (COD), biological oxyge...

journal_title：Bioprocess and biosystems engineering

authors： Oh JH,Park J,Ellis TG

更新日期：2015-02-01 00:00:00

abstract：:A novel suspended carrier with porous surface was firstly prepared by coating a sponge on the inside and outside of a hard polyethylene ring. Herein the effects of the sponge thickness (0, 2, 4, 6 mm) and pore size (17, 45, 85 pores per inch, ppi) on the performance of the start-up stage in moving bed biofilm reactor ...

journal_title：Bioprocess and biosystems engineering

authors： Chen X,Kong L,Wang X,Tian S,Xiong Y

更新日期：2015-02-01 00:00:00

abstract：:To obtain the soluble production of recombinant NovQ, it has been constructed into the pET28a system. Unfortunately, NovQ was mostly accumulated as inclusion bodies and existed in insoluble fractions of E. coli cell lysate. Four partners, namely His6, TrxA, GST and MBP, were investigated in fusion expression and co-ex...

journal_title：Bioprocess and biosystems engineering

authors： Ni W,Liu H,Wang P,Wang L,Sun X,Wang H,Zhao G,Zheng Z

更新日期：2019-03-01 00:00:00