Abstract:
:Formulation conditions have a significant influence on the degree of freeze/thaw (FT) stress-induced protein instabilities. Adding cryoprotectants might stabilize the induced FT stress instabilities. However, a simple preservation of protein stability might be insufficient and further methods are necessary. This study aims to evaluate the addition of a heat cycle following FT application as a function of different cryoprotectants with lysozyme as exemplary protein. Sucrose and glycerol were shown to be the most effective cryoprotectants when compared to PEG200 and Tween20. In terms of heat-induced reversibility of aggregates, glycerol showed the best performance followed by sucrose, NaCl and Tween20 systems. The analysis was performed using a novel approach to visualize complex interplays by a clustering and data reduction scheme. In addition, solubility and structural integrity were measured and confirmed the obtained results.
journal_name
Bioprocess Biosyst Engjournal_title
Bioprocess and biosystems engineeringauthors
Wöll AK,Hubbuch Jdoi
10.1007/s00449-020-02327-3subject
Has Abstractpub_date
2020-07-01 00:00:00pages
1309-1327issue
7eissn
1615-7591issn
1615-7605pii
10.1007/s00449-020-02327-3journal_volume
43pub_type
杂志文章abstract::The hydrolase (Thermobifida fusca hydrolase; TfH) from T. fusca was produced in Escherichia coli as fusion protein using the OmpA leader sequence and a His(6) tag. Productivity could be raised more than 100-fold. Both batch and fed-batch cultivations yield comparable cell specific productivities whereas volumetric pro...
journal_title:Bioprocess and biosystems engineering
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doi:10.1007/s00449-006-0069-9
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journal_title:Bioprocess and biosystems engineering
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doi:10.1007/s00449-007-0161-9
更新日期:2008-01-01 00:00:00
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doi:10.1007/s00449-014-1261-y
更新日期:2015-02-01 00:00:00
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journal_title:Bioprocess and biosystems engineering
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doi:10.1007/s00449-009-0405-y
更新日期:2010-09-01 00:00:00
abstract::In this study, the modulation of enzymatic biocatalysts were developed by the use of lipase B from Candida antarctica covalently immobilized on an eco-friendly support, cashew apple bagasse, activated with 10% glycidol-ethylenediamine-glutaraldehyde (GEG) under different immobilization strategies (5 mM or 100 mM ionic...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
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更新日期:2020-12-01 00:00:00
abstract::In this study, aiming to improve the economic feasibility of acetone-butanol-ethanol (ABE) fermentation process, generate valuable products and extend the product chain, esterification catalyzed by Candida sp. 99-125 cells was hybrid with the ABE fermentation-gas-stripping integration system. The gas-stripping condens...
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pub_type: 杂志文章
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更新日期:2018-09-01 00:00:00
abstract::Enzymatic modification of starch using long chain fatty acid makes it thermoplastic suitable for a myriad of industrial applications. An industrial lipase preparation produced by Burkholderia cepacia (lipase PS) was used for modification of cassava starch with two acyl donors, lauric acid and palmitic acid. Reactions ...
journal_title:Bioprocess and biosystems engineering
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更新日期:2006-06-01 00:00:00
abstract::The use of fed-batch extractive fermentation can overcome inhibitory effects caused by the substrate and ethanol to the yeast cells, since it allows regulate the substrate concentration and remove the product as it is produced. The present study describes the modelling and experimental validation of ethanol production...
journal_title:Bioprocess and biosystems engineering
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abstract::A two-phase dispersion technique, termed emulsification-internal gelation, is proposed for encapsulation of activated sludge in gellan gum microbeads. The influence of emulsion parameters on size distribution of microbeads was investigated. Mean diameter of microbeads varied within a range of 34-265 microm as a descen...
journal_title:Bioprocess and biosystems engineering
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doi:10.1007/s00449-004-0360-6
更新日期:2004-07-01 00:00:00
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journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-014-1317-z
更新日期:2015-04-01 00:00:00
abstract::Polyunsaturated fatty acids (PUFAs) are valuable ingredients in the food and pharmaceutical products due to their beneficial influence on human health. Most studies paid attention on the production of PUFAs from oleaginous micro-organisms but seldom on the comparative proteomics of cells. In the study, three methods (...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-015-1467-7
更新日期:2015-12-01 00:00:00
abstract::An engineered Escherichia coli strain was developed for enhanced isoprene production using D-galactose as substrate. Isoprene is a valuable compound that can be biosynthetically produced from pyruvate and glyceraldehyde-3-phosphate (G3P) through the methylerythritol phosphate pathway (MEP). The Leloir and De Ley-Doudo...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-014-1228-z
更新日期:2014-12-01 00:00:00
abstract::The present study aimed to determine the degradation and transformation of three-ring PAHs phenanthrene and anthracene by Cryptococcus sp. MR22 and Halomonas sp. BR04 under halophilic conditions. The growth progress of Cryptococcus sp. MR22 and Halomonas sp. BR04 on anthracene and phenanthrene was monitored by colony-...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-020-02415-4
更新日期:2020-12-01 00:00:00
abstract::The influence of sugars in culture media on the kinetics of the mono- and disaccharide uptake and cell growth behavior was studied by mid-infrared spectroscopy using a Fourier transform infrared spectrometer (FT-IR) equipped with an attenuate total reflection accessory (ATR). We performed the plant cell cultivation wi...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-007-0142-z
更新日期:2007-11-01 00:00:00
abstract::This study assessed the simultaneous nitrification and denitrification processes and remaining organic matter removal from anaerobic reactor effluent treating wastewater in a single reactor. A structured-bed reactor, with polyurethane foam as support media, was subjected to intermittent aeration and effluent recircula...
journal_title:Bioprocess and biosystems engineering
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doi:10.1007/s00449-018-1940-1
更新日期:2018-08-01 00:00:00
abstract::Naturally mevalonate-resistant acetogen Clostridium sp. MT1243 produced only 425 mM acetate during syngas fermentation. Using Clostridium sp. MT1243 we engineered biocatalyst selectively producing mevalonate from synthesis gas or CO₂/H₂ blend. Acetate production and spore formation were eliminated from Clostridium sp....
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-013-0991-6
更新日期:2014-02-01 00:00:00
abstract::Bio-catalytic in vitro multistep reactions can be combined in a single step in one pot by optimizing multistep reactions under identical reaction condition. Using this analogy, the process of making PEGylated insulin, IN-105, was simplified. Instead of taking the purified active insulin bulk powder as the starting mat...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-012-0722-4
更新日期:2012-10-01 00:00:00
abstract::Calcitriol is an important drug used for treating osteoporosis, which can be produced from vitamin D3. The current method of producing calcitriol from vitamin D3 during cultivation of microbial cells results in low yields of calcitriol and high purification costs. Therefore, in this study, the steps of cell cultivatio...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-015-1368-9
更新日期:2015-07-01 00:00:00
abstract::Freshwater sources are limited and access to clean water is an acute challenge in recent decades. The sustainable water treatments methods are need of time and water desalination is one of the most interesting technology. Most desalination technologies are required high energy input while Microbial Desalination Cells ...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-020-02433-2
更新日期:2021-01-01 00:00:00
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journal_title:Bioprocess and biosystems engineering
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abstract::Exogenous enzymes are extraneous enzymes that are not intrinsic to the subject. The exogenous enzyme industry has been rapidly developing recently. Successful application of recombinant DNA amplification, high-efficiency expression, and immobilization technology to genetically engineered bacteria provides a rich sourc...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章,评审
doi:10.1007/s00449-020-02463-w
更新日期:2020-11-04 00:00:00
abstract::This study introduces an effective method to deposit polypyrrole (PPy) on graphite felt (GF) as anode to improve the start-up performance of microbial fuel cells (MFCs). The results of scanning electron microscope (SEM) and electrochemical testing reveal that polypyrrole is able to improve the electrical conductivity ...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-019-02238-y
更新日期:2020-03-01 00:00:00
abstract::To improve the performance of covalently immobilized penicillin acylase (PA), the immobilization was carried out in mesocellular silica foams (MCFs) using p-benzoquinone as cross linker. The characterizations of the immobilized enzyme were studied carefully. The results showed that the relative activity of the immobil...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-007-0189-x
更新日期:2008-08-01 00:00:00
abstract::Bacterial cellulose (BC) exhibits unique properties such as high purity compared to plant-based cellulose; however, commercial production of BC has remained a challenge, primarily due to the strain properties of cellulose-producing bacteria. Herein, we developed a functional and stable BC production system in genetica...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-017-1864-1
更新日期:2018-02-01 00:00:00
abstract::During ribonucleic acid fermentation, the fermentative processes were researched at pH controlled at 4.0 and under natural conditions. Unstructured models in a 50-L airlift fermentor were established for batch RNA production at pH 4.0 using the Verhulst equation for microbial growth, the Luedeking-Piret equation for p...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-011-0580-5
更新日期:2012-03-01 00:00:00
abstract::Polyhydroxybutyrate (PHB) is a biodegradable bioplastic that is comparable with many petroleum-based plastics in terms of mechanical properties and is highly biocompatible. Lignocellulosic biomass conversion into PHB can increase profit and add sustainability. Glucose, xylose and arabinose are the main monomer sugars ...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-020-02434-1
更新日期:2021-01-01 00:00:00
abstract::Commercial lipase from Thermomyces lanuginosus has been immobilized on glutaraldehyde-activated rice husk particles via covalent attachment. It was reached maximum immobilized protein concentration of 27.5 ± 1.8 mg g-1 of dry support using the initial protein loading of 40 mg g-1 of support. The immobilized biocatalys...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-018-1929-9
更新日期:2018-07-01 00:00:00
abstract::A new method for immobilization of acetylcholinesterase (AChE) to alginate gel beads by activating the carbonyl groups of alginate using carbodiimide coupling agent has been successfully developed. Maximum reaction rate (V (max)) and Michaelis-Menten constant (K (m)) were determined for the free and binary immobilized...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-006-0106-8
更新日期:2007-03-01 00:00:00
abstract::This paper presents a methodology for the determination of reaction rate constants for nitrifying bacteria and their mean population percentage in biomass in an alternating oxidation ditch system. The method used is based on the growth rate equations of the ASM1 model (IWA) (Henze et al. in Activated sludge models ASM...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-010-0446-2
更新日期:2011-01-01 00:00:00