Recovery of electric energy from formate by using a recombinant strain of Escherichia coli.

Abstract:

:Recombinant Escherichia coli cells were applied for the recovery of electric energy from formate. Initially, the fdh gene, which encodes formate dehydrogenase (FDH) of Mycobacterium vaccae, was introduced into E. coli cells to allow efficient degradation of formate. The constructed microbial fuel cell (MFC) with E. coli BW25113 cells carrying fdh gene showed appreciable generation of current density in the presence of formate as a substrate. Current density and polarization curves revealed that the performance of MFC under examined conditions was limited by the electron transfer from bulk liquid to the electrode surface; accordingly, agitation resulted in an increase in the current density and achieved a coulombic efficiency of 21.7 % on the basis of formate consumed. Thus, gene recombination enables E. coli cells to utilize formate as a fuel for MFC.

journal_name

Bioprocess Biosyst Eng

authors

Ojima Y,Kawata T,Matsuo N,Nishinoue Y,Taya M

doi

10.1007/s00449-014-1179-4

subject

Has Abstract

pub_date

2014-10-01 00:00:00

pages

2005-8

issue

10

eissn

1615-7591

issn

1615-7605

journal_volume

37

pub_type

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