Abstract：
:Chemical modification of lysine residues in Candida rugosa lipase (CRL) was carried out using five different functional ionic liquids, and about 15.4-25.0 % of the primary amino groups of lysine were modified. Enzymatic properties of the native and modified CRLs were investigated in olive oil hydrolysis reaction. Improved thermal stability, catalytic activity in organic solvents, and adaptability to temperature and pH changes were achieved compared with the native enzyme. CRL modified by [choline][H2PO4] showed the best results, bearing a maximum improvement of 16.7 % in terms of relative activity, 5.2-fold increase in thermostability (after incubation at 45 °C for 5 h), and 2.3-fold increase in activity in strong polar organic solvent (80 % dimethyl sulfoxide) compared with the native enzyme. The results of ultraviolet, circular dichroism and fluorescence spectroscopy suggested that the change of the secondary and tertiary structures of CRL caused by the chemical modification resulted in the enhancement of enzymatic performance. The modification of CRL with functional ionic liquids was proved to be a novel and efficient method for improving the enzymatic properties of CRL.
journal_name
Bioprocess Biosyst Engjournal_title
Bioprocess and biosystems engineeringauthors
Hu Y,Yang J,Jia R,Ding Y,Li S,Huang Hdoi
10.1007/s00449-014-1134-4subject
Has Abstractpub_date
2014-08-01 00:00:00pages
1617-26issue
8eissn
1615-7591issn
1615-7605journal_volume
37pub_type
杂志文章abstract：:Organic matters are directly converted to electricity by microorganisms in microbial fuel cells (MFC). Modeling the performance of MFC sheds light on the behavior of MFC in various operational conditions (e.g. pH and temperature). In the present research, three voltage losses were considered for modeling of the MFC po...
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