Abstract：
:A whole-cell biocatalytic process for uridine 5'-monophosphate (UMP) production from orotic acid by Saccharomyces cerevisiae was developed. To rationally redistribute the metabolic flux between glycolysis and pentose phosphate pathway, statistical methods were employed first to find out the critical factors in the process. NaH(2)PO(4), MgCl(2) and pH were found to be the important factors affecting UMP production significantly. The levels of these three factors required for the maximum production of UMP were determined: NaH(2)PO(4) 22.1 g/L; MgCl(2) 2.55 g/L; pH 8.15. An enhancement of UMP production from 6.12 to 8.13 g/L was achieved. A significant redistribution of metabolic fluxes was observed and the underlying mechanism was discussed.
journal_name
Bioprocess Biosyst Engjournal_title
Bioprocess and biosystems engineeringauthors
Liu D,Chen Y,Li A,Xie J,Xiong J,Bai J,Chen X,Niu H,Zhou T,Ying Hdoi
10.1007/s00449-011-0653-5subject
Has Abstractpub_date
2012-06-01 00:00:00pages
729-37issue
5eissn
1615-7591issn
1615-7605journal_volume
35pub_type
杂志文章abstract：:The performance of down-flow hanging sponge (DHS) system treating UASB reactor effluent at different sponge volume of 38.2, 28.7, 19.1 and 9.6% was investigated. The hydraulic retention time (HRT) and flow rate were kept constant at 2.6 h and 0.46 m(3)/day, respectively. The results obtained indicated that reducing th...
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journal_title：Bioprocess and biosystems engineering
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journal_title：Bioprocess and biosystems engineering
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更新日期：2003-03-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
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journal_title：Bioprocess and biosystems engineering
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journal_title：Bioprocess and biosystems engineering
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journal_title：Bioprocess and biosystems engineering
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journal_title：Bioprocess and biosystems engineering
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-012-0684-6
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
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journal_title：Bioprocess and biosystems engineering
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