Covalent immobilization of Kluyveromyces fragilis β-galactosidase on magnetic nanosized epoxy support for synthesis of galacto-oligosaccharide.

Abstract:

:The novel magnetic nanobeads with epoxy groups on the surface were prepared from glycidyl methacrylate (GMA), ethylene glycol dimethacrylate (EGDMA) and hydroxyethyl methacrylate (HEMA) via emulsifier-free emulsion polymerisation, and they were characterized by scanning electron microscopy and vibrating sample magnetometer. The magnetic poly(GMA-EDGMA-HEMA) nanobeads were used as support for covalent immobilization of Kluyveromyces fragilis β-galactosidase, the maximum amount enzyme attached onto the support was 145.6 mg/g with activity recovery of 72.6%. The loading capacity of this novel support for K. fragilis β-galactosidase was improved 2.6-folds compared with Eupergit® C (commercial epoxy support). The immobilized K. fragilis β-galactosidase exhibited high catalytic activity for the reaction of galacto-oligosaccharide (GOS) synthesis, and a total of 2,240 g GOS were produced per gram of immobilized enzyme during consecutive batch reaction of 10 times. The immobilized biocatalyst retained 81.5% of its original activity after 10 reaction cycles.

journal_name

Bioprocess Biosyst Eng

authors

Liu H,Liu J,Tan B,Zhou F,Qin Y,Yang R

doi

10.1007/s00449-012-0716-2

subject

Has Abstract

pub_date

2012-10-01 00:00:00

pages

1287-95

issue

8

eissn

1615-7591

issn

1615-7605

journal_volume

35

pub_type

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