Bioconversion of waste office paper to hydrogen using pretreated rumen fluid inoculum.

Abstract:

:In this study, a microbial consortium from an acid-treated rumen fluid was used to improve the yields of H2 production from paper residues in batch reactors. The anaerobic batch reactors, which contained paper and cellulose, were operated under three conditions: (1) 0.5 g paper/L, (2) 2 g paper/L, and (3) 4 g paper/L. Cellulase was added to promote the hydrolysis of paper to soluble sugars. The H2 yields were 5.51, 4.65, and 3.96 mmol H2/g COD, respectively, with substrate degradation ranging from 56 to 65.4 %. Butyric acid was the primary soluble metabolite in the three reactors, but pronounced solventogenesis was detected in the reactors incubated with increased paper concentrations (2.0 and 4.0 g/L). A substantial prevalence of Clostridium acetobutylicum (99 % similarity) was observed in the acid-treated rumen fluid, which has been recognized as an efficient H2-producing strain in addition to ethanol and n-butanol which were also detected in the reactors.

journal_name

Bioprocess Biosyst Eng

authors

Botta LS,Ratti RP,Sakamoto IK,Ramos LR,Silva EL,Varesche MB

doi

10.1007/s00449-016-1663-0

subject

Has Abstract

pub_date

2016-12-01 00:00:00

pages

1887-1897

issue

12

eissn

1615-7591

issn

1615-7605

pii

10.1007/s00449-016-1663-0

journal_volume

39

pub_type

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