Abstract：
:Oxygen supply is crucial in industrial application of microbial systems, such as Corynebacterium glutamicum, but oxygen transfer is often neglected in early strain characterizations, typically done under aerobic conditions. In this work, a new procedure for oxygen transfer screening is presented, assessing the impact of maximum oxygen transfer conditions (OTRmax) within microtiter plate-based cultivation for enhanced throughput. Oxygen-dependent growth and productivity were characterized for C. glutamicum ATCC13032 and C. glutamicum DM1933 (lysine producer). Biomass and lysine product yield are affected at OTRmax below 14 mmol L(-1) h(-1) in a standardized batch process, but not by further increase of OTRmax above this threshold value indicating a reasonable tradeoff between power input and oxygen transfer capacity OTRmax. The described oxygen transfer screening allows comparative determination of metabolic robustness against oxygen transfer limitation and serves identification of potential problems or opportunities later created during scale-up.
journal_name
Bioprocess Biosyst Engjournal_title
Bioprocess and biosystems engineeringauthors
Käß F,Prasad A,Tillack J,Moch M,Giese H,Büchs J,Wiechert W,Oldiges Mdoi
10.1007/s00449-014-1234-1subject
Has Abstractpub_date
2014-12-01 00:00:00pages
2567-77issue
12eissn
1615-7591issn
1615-7605journal_volume
37pub_type
杂志文章abstract：:Two laboratory-scale expanded granular sludge bed (EGSB) reactors were operated at 18 and 25 °C, respectively, for the treatment of synthetic wastewater composed of ethanol and 1-methoxy-2-propanol (M2P) in a mass ratio of 4:1. Reactors were operated first with continuous wastewater supply and after with discontinuous...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
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journal_title：Bioprocess and biosystems engineering
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doi：10.1007/s00449-006-0060-5
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更新日期：2011-09-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
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journal_title：Bioprocess and biosystems engineering
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journal_title：Bioprocess and biosystems engineering
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-010-0501-z
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更新日期：2003-07-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章,评审
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更新日期：2019-06-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
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doi：10.1007/s00449-015-1496-2
更新日期：2016-01-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
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更新日期：2015-02-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
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doi：10.1007/s00449-004-0381-1
更新日期：2004-12-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
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doi：10.1007/s00449-013-1091-3
更新日期：2014-06-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
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doi：10.1007/s00449-012-0874-2
更新日期：2013-09-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-015-1357-z
更新日期：2015-06-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
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journal_title：Bioprocess and biosystems engineering
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journal_title：Bioprocess and biosystems engineering
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journal_title：Bioprocess and biosystems engineering
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journal_title：Bioprocess and biosystems engineering
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更新日期：2013-06-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
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更新日期：2018-10-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
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journal_title：Bioprocess and biosystems engineering
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更新日期：2013-10-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
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