Dielectrophoretic separation of monocytes from cancer cells in a microfluidic chip using electrode pitch optimization.

Abstract:

:This study proposes a microfluidic device capable of separating monocytes from a type of cancer cell that is called T-cell acute lymphoblastic leukemia (RPMI-8402) in a continuous flow using negative and positive dielectrophoretic forces. The use of both the hydrodynamic and dielectrophoretic forces allows the separation of RPMI-8402 from monocytes based on differences in their intrinsic electrical properties and sizes. The specific crossover frequencies of monocytes and RPMI-8402 cells have been obtained experimentally. The optimum ranges of electrode pitch-to-channel height ratio at the cross sections with different electrode widths have been generally calculated by numerical simulations of the gradients of the electric field intensities and calculation their effective values (root-mean-square). In the device, the cell sorting has been conducted empirically, and then, the separation performance has been evaluated by analyzing the images before and after dielectrophoretic forces applied to the cells. In this work, the design of a chip with 77 μm gold-titanium electrode pitch was investigated to achieve high purity of monocytes of 95.2%. The proposed device can be used with relatively low applied voltages, as low as 16.5 V (peak to peak). Thus, the design can be used in biomedical diagnosis and chemical analysis applications as a lab-on-chip platform. Also, it can be used for the separation of biological cells such as bacteria, RNA, DNA, and blood cells.

journal_name

Bioprocess Biosyst Eng

authors

Zahedi Siani O,Zabetian Targhi M,Sojoodi M,Movahedin M

doi

10.1007/s00449-020-02349-x

subject

Has Abstract

pub_date

2020-09-01 00:00:00

pages

1573-1586

issue

9

eissn

1615-7591

issn

1615-7605

pii

10.1007/s00449-020-02349-x

journal_volume

43

pub_type

杂志文章
  • Immobilization of Amano lipase AK from Pseudomonas fluorescens on different types of chitosan-containing supports: use in the kinetic resolution of rac-indanol.

    abstract::Amano lipase AK from P. fluorescens was immobilized on different types of chitosan-containing supports. Chitosan lower molecular weight (2.5%), chitosan lower molecular weight/sodium alginate (2.5%/2.5%) and chitosan lower molecular weight/carrageenan (2.5%/2.5%) allowed the highest values of immobilization yields (IY...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-020-02487-2

    authors: de Sousa Fonseca T,de Oliveira UMF,de Oliveira MDCF,de Lemos TLG,da Silva MR,Rios NS,Gonçalves LRB,de Mattos MC

    更新日期:2021-01-03 00:00:00

  • Evaluation of ethanol production and bioadsorption of heavy metals by various red seaweeds.

    abstract::The objective of this study was to evaluate ethanol production and bioadsorption with four red seaweeds, Gelidium amansii, Gracilaria verrucosa, Kappaphycus alvarezii and Eucheuma denticulatum. To produce ethanol, thermal acid hydrolysis, enzymatic saccharification and fermentation was carried out. After pretreatment,...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-016-1571-3

    authors: Sunwoo IY,Ra CH,Jeong GT,Kim SK

    更新日期:2016-06-01 00:00:00

  • Use of the effluent from biogas production for cultivation of Spirulina.

    abstract::The effluent from the biogas process was tested as a nutrient source during cultivation of the protein-rich and edible microalgae Spirulina (Arthrospira platensis) and compared with conventional Spirulina medium. Equal biomass production was observed until late exponential phase and no significant differences could be...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-016-1726-2

    authors: Hultberg M,Lind O,Birgersson G,Asp H

    更新日期:2017-04-01 00:00:00

  • Biosynthesis of high-purity γ-MnS nanoparticle by newly isolated Clostridiaceae sp. and its properties characterization.

    abstract::MnS is a p-type semiconductor with both antiferromagnetism and wide band gap, endowing it potential applications for short wavelength optoelectronic devices, solar cells and luminescent materials. Despite successful biosynthesis of nano CdS, PbS and ZnS with extremely low solubility product, there have been no reports...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-014-1261-y

    authors: Liu X,Wang J,Yue L,Xin B,Chen S,Dai J,Wang R,Wang Y

    更新日期:2015-02-01 00:00:00

  • Mechanisms of the inhibition of enzymatic hydrolysis of waste pulp fibers by calcium carbonate and the influence of nonionic surfactant for mitigation.

    abstract::Recycled paper mills produce large quantities of fibrous rejects and fines which are usually sent to landfills as solid waste. These cellulosic materials can be enzymatically hydrolyzed into sugars for the production of biofuels and biomaterials. Paper mill wastes also contain large amounts of calcium carbonate which ...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-017-1745-7

    authors: Min BC,Ramarao BV

    更新日期:2017-06-01 00:00:00

  • Towards efficient enzymatic conversion of D-galactose to D-tagatose: purification and characterization of L-arabinose isomerase from Lactobacillus brevis.

    abstract::L-arabinose isomerase (L-AI) (EC 5. 3. 1. 4. L-AI) that mediates the isomerization of D-galactose to D-tagatose was isolated from Lactobacillus brevis (MF 465792), and was further purified and characterized. Pure enzyme with molecular weight of 60.1 kDa was successfully obtained after the purification using Native-PAG...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-018-2018-9

    authors: Du M,Zhao D,Cheng S,Sun D,Chen M,Gao Z,Zhang C

    更新日期:2019-01-01 00:00:00

  • Sequential pretreatment strategies under mild conditions for efficient enzymatic hydrolysis of wheat straw.

    abstract::This work studies the sequential execution of dilute sulfuric acid (DAP) (0.1-0.75 %, v/v) and dilute sodium hydroxide (AKP) (0.25-3 %, w/v) [i.e., DAP followed by AKP (DAP+AKP) and vice versa (AKP+DAP)] at low temperatures (<121 °C) and short reaction times (5-60 min) for maximizing sugar recovery in the enzymatic hy...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-015-1355-1

    authors: Sanchez A,Gil JC,Rojas-Rejón OA,de Alba AP,Medina A,Flores R,Puente R

    更新日期:2015-06-01 00:00:00

  • Feasibility of attached cultivation for polysaccharides production by Porphyridium cruentum.

    abstract::Porphyridium cruentum is one of the most valued microalgae species able to produce both pigments and exopolysaccharides. Conventional liquid suspended cultivation in ponds and photobioreactors show its disadvantages in lower cultivation efficiency and higher stirring power consumption due to the high viscosity of the ...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-016-1676-8

    authors: Lutzu GA,Zhang L,Zhang Z,Liu T

    更新日期:2017-01-01 00:00:00

  • Measuring the adhesion limit of fibronectin for fibroblasts with a narrow-gap rotational rheometer.

    abstract::We study the adhesion limit of 3T6 fibroblasts, cultured in Dulbecco's modified Eagle's medium with 10% fetal bovine serum at 37 °C and 5% CO2, with a narrow-gap rotational rheometer in the parallel-disk configuration. Reducing the uncertainty in gap width to about 1 µm allows studying the cells at narrow gaps, which ...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-017-1868-x

    authors: Dakhil H,Do H,Hübner H,Wierschem A

    更新日期:2018-03-01 00:00:00

  • Cauliflower waste utilization for sustainable biobutanol production: revelation of drying kinetics and bioprocess development.

    abstract::Efficient yet economic production of biofuel(s) using varied second-generation feedstock needs to be explored in the current scenario to cope up with global fuel demand. Hence, the present study was performed to reveal the use of cauliflower waste for acetone-butanol-ethanol (ABE) production using Clostridium acetobut...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-017-1806-y

    authors: Khedkar MA,Nimbalkar PR,Chavan PV,Chendake YJ,Bankar SB

    更新日期:2017-10-01 00:00:00

  • Efficient utilization of Eucheuma denticulatum hydrolysates using an activated carbon adsorption process for ethanol production in a 5-L fermentor.

    abstract::A total monosaccharide concentration of 37.8 g/L and 85.9% conversion from total fermentable monosaccharides of 44.0 g/L from 110 g dw/L Eucheuma denticulatum slurry were obtained by thermal acid hydrolysis and enzymatic saccharification. Subsequent adsorption treatment to remove 5-hydroxymethylfurfural (5-HMF) using ...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-016-1705-7

    authors: Ra CH,Kim MJ,Jeong GT,Kim SK

    更新日期:2017-03-01 00:00:00

  • Poly(styrene-divinylbenzene) beads surface functionalized with di-block polymer grafting and multi-modal ligand attachment: performance of reversibly immobilized lipase in ester synthesis.

    abstract::Fibrous poly(styrene-b-glycidylmethacrylate) brushes were grafted on poly(styrene-divinylbenzene) (P(S-DVB)) beads using surface-initiated atom transfer radical polymerization. Tetraethyldiethylenetriamine (TEDETA) ligand was incorporated on P(GMA) block. The ligand attached beads were used for reversible immobilizati...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-011-0523-1

    authors: Bayramoglu G,Karagoz B,Altintas B,Arica MY,Bicak N

    更新日期:2011-08-01 00:00:00

  • Characterization of Pb2+ biosorption from aqueous solution by Rhodotorula glutinis.

    abstract::The yeast Rhodotorula glutinis was examined for its ability to remove Pb(2+) from aqueous solution. Within 10 min of contact, Pb(2+) sorption reached nearly 80% of the total Pb(2+) sorption. The optimum initial pH value for removal of Pb(2+ )was 4.5-5.0. The percentage sorption increased steeply with the biomass conce...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-002-0315-8

    authors: Cho DH,Kim EY

    更新日期:2003-03-01 00:00:00

  • Enzymatic modification of cassava starch by bacterial lipase.

    abstract::Enzymatic modification of starch using long chain fatty acid makes it thermoplastic suitable for a myriad of industrial applications. An industrial lipase preparation produced by Burkholderia cepacia (lipase PS) was used for modification of cassava starch with two acyl donors, lauric acid and palmitic acid. Reactions ...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-006-0060-5

    authors: Rajan A,Abraham TE

    更新日期:2006-06-01 00:00:00

  • Oxidases from mate tea leaves (Ilex paraguariensis): extraction optimization and stability at low and high temperatures.

    abstract::Mate (Ilex paraguariensis) is an important natural product in the economic and cultural context of Brazil. Peroxidase and polyphenol oxidase have been responsible for quality deterioration and browning in mate. The objective of this work was to investigate a methodology of extraction and enzymatic activity determinati...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-007-0196-y

    authors: Ceni GC,Baldissera EM,Antunes OA,Vladimir Oliveira J,Dariva C,de Oliveira D

    更新日期:2008-10-01 00:00:00

  • Production of a recombinant polyester-cleaving hydrolase from Thermobifida fusca in Escherichia coli.

    abstract::The hydrolase (Thermobifida fusca hydrolase; TfH) from T. fusca was produced in Escherichia coli as fusion protein using the OmpA leader sequence and a His(6) tag. Productivity could be raised more than 100-fold. Both batch and fed-batch cultivations yield comparable cell specific productivities whereas volumetric pro...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-006-0069-9

    authors: Dresler K,van den Heuvel J,Müller RJ,Deckwer WD

    更新日期:2006-08-01 00:00:00

  • The study of neural network-based controller for controlling dissolved oxygen concentration in a sequencing batch reactor.

    abstract::The design and development of the neural network (NN)-based controller performance for the activated sludge process in sequencing batch reactor (SBR) is presented in this paper. Here we give a comparative study of various neural network (NN)-based controllers such as the direct inverse control, internal model control ...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-005-0031-2

    authors: Azwar,Hussain MA,Ramachandran KB

    更新日期:2006-03-01 00:00:00

  • Simultaneous acetone-butanol-ethanol fermentation, gas stripping, and full-cell-catalyzed esterification for effective production of butyl oleate.

    abstract::In this study, aiming to improve the economic feasibility of acetone-butanol-ethanol (ABE) fermentation process, generate valuable products and extend the product chain, esterification catalyzed by Candida sp. 99-125 cells was hybrid with the ABE fermentation-gas-stripping integration system. The gas-stripping condens...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-018-1960-x

    authors: Chen C,Cai D,Chen H,Cai J,Sun G,Qin P,Chen B,Zhen Y,Tan T

    更新日期:2018-09-01 00:00:00

  • Enhanced succinic acid production under acidic conditions by introduction of glutamate decarboxylase system in E. coli AFP111.

    abstract::Biological synthesis of succinic acid at low pH values was favored since it not only decreased investment cost but also simplified downstream purification process. In this study, the feasibility of using glutamate decarboxylase system to improve succinic acid production of Escherichia coli AFP111, a succinate-producin...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-016-1720-8

    authors: Wu M,Li X,Guo S,Lemma WD,Zhang W,Ma J,Jia H,Wu H,Jiang M,Ouyang P

    更新日期:2017-04-01 00:00:00

  • Factors affecting simultaneous nitrification and denitrification (SND) in a moving bed sequencing batch reactor (MBSBR) system as revealed by microbial community structures.

    abstract::The effects of biological factors including dissolved oxygen (DO), pH, carbon/nitrogen (C/N) and hydraulic retention times (HRT) on the performance of simultaneous nitrification and denitrification (SND) in a moving bed sequencing batch reactor (MBSBR) were investigated. A low DO was found to be advantageous to the SN...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-020-02374-w

    authors: Wang J,Rong H,Cao Y,Zhang C

    更新日期:2020-10-01 00:00:00

  • A tracer study for assessing the interactions between hydraulic retention time and transport processes in a wetland system for nutrient removal.

    abstract::In this study, a new-generation subsurface upflow wetland (SUW) system packed with the unique sorption media was introduced for nutrient removal. To explore the interface between hydraulic and environmental performance, a tracer study was carried out in concert with a transport model to collectively provide hydraulic ...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-011-0578-z

    authors: Chang NB,Xuan Z,Wanielista MP

    更新日期:2012-03-01 00:00:00

  • Microbial transformation and sorption of anthracene in liquid culture.

    abstract::Armillaria sp. F022, a white-rot fungus isolated from decayed wood in tropical rain forest was used to biodegrade anthracene in cultured medium. The percentage of anthracene removal by Armillaria sp. F022 reached 13 % after 7 days and at the end of the experiment, anthracene removal level was at 87 %. The anthracene r...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-012-0850-x

    authors: Hadibarata T,Zubir MM,Rubiyatno,Chuang TZ

    更新日期:2013-09-01 00:00:00

  • Changes in respiratory quinone profiles of enhanced biological phosphorus removal activated sludge under different influent phosphorus/carbon ratio conditions.

    abstract::Changes in the microbial community of an enhanced biological phosphorus removal (EBPR) activated sludge system under different influent phosphorus/carbon (P/C) ratio conditions were investigated through evaluation of population respiratory quinone profiles. A total of 13 types of respiratory quinone homologs consistin...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-006-0065-0

    authors: Ahn J,Lee M,Kwon H

    更新日期:2006-08-01 00:00:00

  • Highly efficient synthesis of boldenone from androst-4-ene-3,17-dione by Arthrobacter simplex and Pichia pastoris ordered biotransformation.

    abstract::Boldenone (BD) is an important steroid hormone drug which is the derivative of testosterone. In this study, an ordered biotransformation method was proposed employing Arthrobacter simplex and recombinant Pichia pastoris with 17β-hydroxysteroid dehydrogenase from Saccharomyces cerevisiae to produce BD from androst-4-en...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-019-02092-y

    authors: Tang R,Shen Y,Wang M,Zhou H,Zhao Y

    更新日期:2019-06-01 00:00:00

  • Medium modification to enhance the formation of bioactive metabolites in shake flask cultures of Antrodia cinnamomea by adding citrus peel extract.

    abstract::Antrodia cinnamomea has recently become a well-known medicinal mushroom in Taiwan. Bioactive compounds found in A. cinnamomea include: polysaccharide, sesquiterpene lactone, steroids and triterpenoids. The aim of this study was to evaluate the feasibility of adding citrus peel extract to enhance the formation of bioac...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-012-0712-6

    authors: Yang FC,Ma TW,Chuang YT

    更新日期:2012-10-01 00:00:00

  • Mathematical model of organic substrate degradation in solid waste windrow composting.

    abstract::Organic solid waste composting is a complex process that involves many coupled physical, chemical and biological mechanisms. To understand this complexity and to ease in planning, design and management of the composting plant, mathematical model for simulation is usually applied. The aim of this paper is to develop a ...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-015-1492-6

    authors: Seng B,Kristanti RA,Hadibarata T,Hirayama K,Katayama-Hirayama K,Kaneko H

    更新日期:2016-01-01 00:00:00

  • Continuous degradation of maltose: improvement in stability and catalytic properties of maltase (α-glucosidase) through immobilization using agar-agar gel as a support.

    abstract::Maltose degrading enzyme was immobilized within agar-agar support via entrapment method due to its industrial utilization. The maximum immobilization efficiency (82.77%) was achieved using 4.0% agar-agar keeping the diameter of bead up to 3.0 mm. The matrix entrapment showed maximum catalytic activity at pH 7.0 and te...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-014-1302-6

    authors: Nawaz MA,Karim A,Aman A,Marchetti R,Qader SA,Molinaro A

    更新日期:2015-04-01 00:00:00

  • Evaluation of multiple fused partners on enhancing soluble level of prenyltransferase NovQ in Escherichia coli.

    abstract::To obtain the soluble production of recombinant NovQ, it has been constructed into the pET28a system. Unfortunately, NovQ was mostly accumulated as inclusion bodies and existed in insoluble fractions of E. coli cell lysate. Four partners, namely His6, TrxA, GST and MBP, were investigated in fusion expression and co-ex...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-018-2050-9

    authors: Ni W,Liu H,Wang P,Wang L,Sun X,Wang H,Zhao G,Zheng Z

    更新日期:2019-03-01 00:00:00

  • Membrane fluidity of halophilic ectoine-secreting bacteria related to osmotic and thermal treatment.

    abstract::In response to sudden decrease in osmotic pressure, halophilic microorganisms secrete their accumulated osmolytes. This specific stress response, combined with physiochemical responses to the altered environment, influence the membrane properties and integrity of cells, with consequent effects on growth and yields in ...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-013-0957-8

    authors: Bergmann S,David F,Clark W,Wittmann C,Krull R

    更新日期:2013-12-01 00:00:00

  • Investigating the potential of locally sourced wastewater as a feedstock of microbial desalination cell (MDC) for bioenergy production.

    abstract::Freshwater sources are limited and access to clean water is an acute challenge in recent decades. The sustainable water treatments methods are need of time and water desalination is one of the most interesting technology. Most desalination technologies are required high energy input while Microbial Desalination Cells ...

    journal_title:Bioprocess and biosystems engineering

    pub_type: 杂志文章

    doi:10.1007/s00449-020-02433-2

    authors: Liaquat R,Mehmood T,Khoja AH,Iqbal N,Ejaz H,Mumtaz S

    更新日期:2021-01-01 00:00:00