Abstract：
:The hydrolase (Thermobifida fusca hydrolase; TfH) from T. fusca was produced in Escherichia coli as fusion protein using the OmpA leader sequence and a His(6) tag. Productivity could be raised more than 100-fold. Both batch and fed-batch cultivations yield comparable cell specific productivities whereas volumetric productivities differ largely. In the fed-batch cultivations final rTfH concentrations of 0.5 g L(-1) could be achieved. In batch cultivations the generated rTfH is translocated to the periplasm wherefrom it is completely released into the extracellular medium. In fed-batch runs most of the produced rTfH remains as soluble protein in the cytoplasm and only a fraction of about 35% is translocated to the periplasm. Migration of periplasmic proteins in the medium is obviously coupled with growth rate and this final transport step possibly plays an important role in product localization and efficacy of the Sec translocation process.
journal_name
Bioprocess Biosyst Engjournal_title
Bioprocess and biosystems engineeringauthors
Dresler K,van den Heuvel J,Müller RJ,Deckwer WDdoi
10.1007/s00449-006-0069-9subject
Has Abstractpub_date
2006-08-01 00:00:00pages
169-83issue
3eissn
1615-7591issn
1615-7605journal_volume
29pub_type
杂志文章abstract：:An environmentally friendly chemical, tetrakis(hydroxymethyl)phosphonium sulfate (THPS), was used as a metabolic uncoupler to reduce sludge production in a pilot-scale anaerobic/anoxic/oxic process. The results show that the addition of THPS (1.08-1.86 mL/m(3) influent) in the sludge return section could reduce waste ...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-013-1022-3
更新日期：2014-03-01 00:00:00
abstract：:Menaquinone homologs (MK-n), that is, MK-4, MK-5, and MK-6, can be produced by the fermentation of Flavobacterium. In this study, we proposed a simple and efficient method for the extraction of menaquinones from wet cells without the process of drying the biomass. Meanwhile, a rapid and effective solution for the sepa...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-017-1851-6
更新日期：2018-01-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-013-0951-1
更新日期：2013-11-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-017-1833-8
更新日期：2017-12-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-009-0311-3
更新日期：2009-10-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-016-1705-7
更新日期：2017-03-01 00:00:00
abstract：:The performance of down-flow hanging sponge (DHS) system treating UASB reactor effluent at different sponge volume of 38.2, 28.7, 19.1 and 9.6% was investigated. The hydraulic retention time (HRT) and flow rate were kept constant at 2.6 h and 0.46 m(3)/day, respectively. The results obtained indicated that reducing th...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-009-0399-5
更新日期：2010-09-01 00:00:00
abstract：:Penicillin G acylase (PGA) from Kluyvera citrophila immobilized on Amberzyml was used for enantioselective hydrolysis of N-phenylacetylated-DL-tert-leucine (N-Phac-DL-Tle) to produce L-tert-leucine (L-Tle). The effects of various organic cosolvents on hydrolysis of N-Phac-DL-Tle have been investigated in aqueous-cosol...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-005-0027-y
更新日期：2006-04-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-012-0874-2
更新日期：2013-09-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-010-0446-2
更新日期：2011-01-01 00:00:00
abstract：:The mammalian pathogen Bordetella bronchiseptica was grown under controlled batch conditions with glutamate as the primary carbon and nitrogen source. First, a Box-Behnken statistical design quantified the effect of Mg, sulfate, and nicotinate on the antigen filamentous hemagglutinin (FHA) formation. Using lactic acid...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-013-0977-4
更新日期：2014-02-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章,评审
doi：10.1007/s00449-008-0223-7
更新日期：2009-01-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-014-1133-5
更新日期：2014-08-01 00:00:00
abstract：:The gold-rhenium (AuRe) composite nanoparticle was prepared by NaBH(4) reduction procedure, and was modified by the aptamer to obtain an AuRe nanoprobe (AuRessDNA) for thrombin. In pH 7.0 Tris-HCl buffer solution and in the presence of salt, the nanoprobe specifically combined with thrombin to form AuRe-aptamer-thromb...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-010-0434-6
更新日期：2010-11-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-010-0439-1
更新日期：2010-11-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-013-0969-4
更新日期：2013-12-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-014-1275-5
更新日期：2015-02-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章,评审
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更新日期：2018-01-01 00:00:00
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doi：10.1007/s00449-015-1357-z
更新日期：2015-06-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00449-015-1365-z
更新日期：2015-07-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-020-02434-1
更新日期：2021-01-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-018-1895-2
更新日期：2018-05-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-015-1368-9
更新日期：2015-07-01 00:00:00
abstract：:In this study, sodium gluconate was applied as a novel carbon source for the fuel ethanol production using an engineered Escherichia coli strain KO11 in batch fermentations. Ethanol and acetic acid were produced as two major products as well as small amount of lactic acid during the fermentation. Compared to the conve...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-013-0938-y
更新日期：2013-11-01 00:00:00
abstract：:Sorghum stalks were evaluated to generate clean sugars; enzymatic hydrolysis efficiency of acid pretreated biomass was significantly low (12.3 g sugar/g pretreated biomass) compared to alkali method (49.1 g sugar/g pretreated biomass). Inhibitory compounds derived from acid pretreatment, such as hydroxymethyl furfural...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章,评审
doi：10.1007/s00449-016-1727-1
更新日期：2017-04-01 00:00:00
abstract：:This work is focused on the characterization of a commercial cellulase in terms of optimum pH and temperature, stability to pH and temperature and affinity of this enzyme to several substrates, determining the Michaelis-Menten parameters. Maximum activity of cellulase was obtained for the temperature range from 40 to ...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-012-0710-8
更新日期：2012-09-01 00:00:00
abstract：:Chemical composition and physical structure of solid substrate have significantly impacts on fermentation performance. The aqueous ammonia was used to pretreat rice straw. Furthermore, the feasibility of pretreatment to improve laccase production was also evaluated in terms of the enzymatic digestibility, chemical str...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-018-02060-y
更新日期：2019-04-01 00:00:00
abstract：:Vitamin B12 is a crucial fine chemical that is widely used in the pharmaceutical, food and chemical industries, and its production solely dependents on microbial fermentation. We previously constructed an artificial vitamin B12 biosynthesis pathway in Escherichia coli, but the yield of the engineered strains was low. ...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-020-02355-z
更新日期：2020-10-01 00:00:00
abstract：:Bacillusfirmus strain 37 produces the cyclomaltodextrin glucanotransferase (CGTase) enzyme and CGTase produces cyclodextrins (CDs) through a starch cyclization reaction. The strategy for the cloning and expression of recombinant CGTase is a potentially viable alternative for the economically viable production of CGTas...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-018-02068-4
更新日期：2019-04-01 00:00:00
abstract：:Large continuous flow bioreactors are often under the influence of noise in the feed stream(s). Prior removal of noise is done by filters based either on specific algorithms or on artificial intelligence. Neither method is perfect. Hybrid filters combine both methods and thereby capitalize on their strengths while min...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-007-0113-4
更新日期：2007-05-01 00:00:00
