Abstract：
:Bacillus sp. BC-001 was first reported as a potent thermotolerant and homofermentative strain for an industrial-scale L-lactate production. In a flask culture, this isolate fermented both glucose and sucrose to lactate with high yield (0.96 and 0.87 g/g) and productivity (2.8 and 2.6 g/L h), respectively. The higher lactate production performance was obtained in the simultaneous saccharification and fermentation of liquefied starch (150.1 g/L final titer, 0.98 g/g yield, 3.2 g/L h productivity) and the fed-batch glucose fermentation (139.9 g/L final titer, 0.96 g/g yield, 2.9 g/L h productivity). Significant increase in lactate productivity (5.5-6.1 g/L h) was obtained from the high/heavy-inoculum seed in the stirred tank fermentor. Both calcium bases and monovalent bases were successfully employed for pH control during lactate fermentation by this isolate resulting in a versatile and simple operation. By the two-phase fermentation using the high/heavy-inoculum seed of BC-001, the fermentation reproducibility was acquired toward the pilot-scale fermentors.
journal_name
Bioprocess Biosyst Engjournal_title
Bioprocess and biosystems engineeringauthors
Thitiprasert S,Kodama K,Tanasupawat S,Prasitchoke P,Rampai T,Prasirtsak B,Tolieng V,Piluk J,Assabumrungrat S,Thongchul Ndoi
10.1007/s00449-017-1833-8subject
Has Abstractpub_date
2017-12-01 00:00:00pages
1787-1799issue
12eissn
1615-7591issn
1615-7605pii
10.1007/s00449-017-1833-8journal_volume
40pub_type
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