Characterization of a commercial cellulase for hydrolysis of agroindustrial substrates.

abstract：:Safe and eco-friendly alternatives to currently used hazardous chemico-physical methods of silver nanoparticles (AgNPs) synthesis are need of time. Rapid, low cost, selective detection of toxic metals in environmental sample is important to take safety action. Toxicity assessment of engineered AgNPs is essential to av...

journal_title：Bioprocess and biosystems engineering

authors： Borase HP,Patil CD,Salunkhe RB,Suryawanshi RK,Salunke BK,Patil SV

更新日期：2014-11-01 00:00:00

abstract：:Salmonella is a major pathogen that causes acute foodborne outbreaks worldwide. Seafood, particularly shellfish, is a proven source of Salmonella spp. infection because many people prefer to eat it raw or lightly cooked. However, traditional identification methods are too time-consuming and complex to detect contamina...

journal_title：Bioprocess and biosystems engineering

authors： Gao W,Huang H,Zhu P,Yan X,Fan J,Jiang J,Xu J

更新日期：2018-05-01 00:00:00

abstract：:The hydrolase (Thermobifida fusca hydrolase; TfH) from T. fusca was produced in Escherichia coli as fusion protein using the OmpA leader sequence and a His(6) tag. Productivity could be raised more than 100-fold. Both batch and fed-batch cultivations yield comparable cell specific productivities whereas volumetric pro...

journal_title：Bioprocess and biosystems engineering

authors： Dresler K,van den Heuvel J,Müller RJ,Deckwer WD

更新日期：2006-08-01 00:00:00

abstract：:Bacillus sp. BC-001 was first reported as a potent thermotolerant and homofermentative strain for an industrial-scale L-lactate production. In a flask culture, this isolate fermented both glucose and sucrose to lactate with high yield (0.96 and 0.87 g/g) and productivity (2.8 and 2.6 g/L h), respectively. The higher l...

journal_title：Bioprocess and biosystems engineering

authors： Thitiprasert S,Kodama K,Tanasupawat S,Prasitchoke P,Rampai T,Prasirtsak B,Tolieng V,Piluk J,Assabumrungrat S,Thongchul N

更新日期：2017-12-01 00:00:00

abstract：:Lysozyme, a type of natural enzyme, has been widely used for bacteriostatic functionalization of various materials due to its efficient and selective antibacterial properties. Herein, we report the preparation and characterization of an eco-friendly antibacterial textile based on the immobilization of lysozyme from ch...

journal_title：Bioprocess and biosystems engineering

authors： Yang W,Zhang N,Wang Q,Wang P,Yu Y

更新日期：2020-09-01 00:00:00

abstract：:Chinese hamster ovary (CHO) cells are the main workhorse in the biopharmaceutical industry for the production of recombinant proteins, such as monoclonal antibodies. To date, a variety of metabolic engineering approaches have been used to improve the productivity of CHO cells. While genetic manipulations are potential...

journal_title：Bioprocess and biosystems engineering

authors： Fouladiha H,Marashi SA,Torkashvand F,Mahboudi F,Lewis NE,Vaziri B

更新日期：2020-08-01 00:00:00

abstract：:A carbon membrane-aerated biofilm reactor (CMABR) was developed to treat synthetic wastewater. Such membrane exhibited a high degree of adhesion and good permeability. Continuous experiments showed that COD and NH4(+)-N removal efficiency were 90 +/- 2 and 92 +/- 4% at removal rates of 35.6 +/- 3.8 g COD/m2 per day an...

journal_title：Bioprocess and biosystems engineering

authors： Liu H,Yang F,Wang T,Liu Q,Hu S

更新日期：2007-07-01 00:00:00

abstract：:Of various metal ions (Ca2+, Cr3+, Cu2+, Fe2+, Mg2+, Mn2+, Ni2+ and Zn2+) added to the culture medium of Agrobacterium tumefaciens at 1 mM, only Ca2+ increased Coenzyme Q10 (CoQ(10)) content in cells without the inhibition of cell growth. In a pH-stat fed-batch culture, supplementation with 40 mM of CaCO3 increased th...

journal_title：Bioprocess and biosystems engineering

authors： Ha SJ,Kim SY,Seo JH,Jeya M,Zhang YW,Ramu T,Kim IW,Lee JK

更新日期：2009-08-01 00:00:00

abstract：:A concept for the determination of concentrations in microchannels using FT-IR spectroscopy in transmission is presented. The fundamental idea of spatially resolved measurements along several measuring points was implemented in a single-channel microreactor. Compared to existing microreactor setups for the analysis of...

journal_title：Bioprocess and biosystems engineering

authors： Fagaschewski J,Sellin D,Wiedenhöfer C,Bohne S,Trieu HK,Hilterhaus L

更新日期：2015-07-01 00:00:00

abstract：:Commercial lipase from Thermomyces lanuginosus has been immobilized on glutaraldehyde-activated rice husk particles via covalent attachment. It was reached maximum immobilized protein concentration of 27.5 ± 1.8 mg g-1 of dry support using the initial protein loading of 40 mg g-1 of support. The immobilized biocatalys...

journal_title：Bioprocess and biosystems engineering

authors： Lima LCD,Peres DGC,Mendes AA

更新日期：2018-07-01 00:00:00

abstract：:Microalgal carotenoids are attractive health ingredients, but their production should be optimized to improve cost-effectiveness. Understanding cellular physiology centered on carotenoid synthesis is the prerequisite for this work. Therefore, systematic correlation analyses were conducted among chlorophyll, carotenoid...

journal_title：Bioprocess and biosystems engineering

authors： Song HG,Byeon SY,Chung GY,Jung SM,Choi JI,Shin HS

更新日期：2018-09-01 00:00:00

abstract：:Laccases are versatile oxidases that are capable of decolorizing various synthetic dyes. Recombinant Bacillus amyloliquefaciens laccase was immobilized as magnetic cross-linked enzyme aggregates (M-CLEAs) for application in dye decolorization. Several parameters influencing the activity recovery were evaluated during ...

journal_title：Bioprocess and biosystems engineering

authors： Wang H,Han S,Wang J,Yu S,Li X,Lu L

更新日期：2020-11-27 00:00:00

abstract：:Two laboratory-scale expanded granular sludge bed (EGSB) reactors were operated at 18 and 25 °C, respectively, for the treatment of synthetic wastewater composed of ethanol and 1-methoxy-2-propanol (M2P) in a mass ratio of 4:1. Reactors were operated first with continuous wastewater supply and after with discontinuous...

journal_title：Bioprocess and biosystems engineering

authors： Lafita C,Penya-roja JM,Gabaldón C

更新日期：2015-11-01 00:00:00

abstract：:A strain designated M866, producing kojic acid with a high yield, was obtained by combining induced mutation using ion beam implantation and ethyl methane sulfonate treatment of a wild type strain of Aspergillus oryzae B008. The amount of kojic acid produced by the strain M866 in a shaking flask was 40.2 g/L from 100 ...

journal_title：Bioprocess and biosystems engineering

authors： Yan S,Tang H,Wang S,Xu L,Liu H,Guo Y,Yao J

更新日期：2014-06-01 00:00:00

abstract：:Infection with Vibrio cholerae is a significant problem in many developing countries. Cultivation of V. cholerae is used in production of cholera toxin B subunit, which is a component in a cholera vaccine. Fed-batch cultivations with V. cholerae in defined media have been conducted and reproducible results were obtain...

journal_title：Bioprocess and biosystems engineering

authors： de Maré L,Andersson L,Hagander P

更新日期：2003-01-01 00:00:00

abstract：:L-Lysine is an essential amino acid that can be produced by chemical processes from fossil raw materials, as well as by microbial fermentation, the latter being a more efficient and environmentally friendly procedure. In this work, the production process of L-lysine-HCl is studied using a systematic approach based on ...

journal_title：Bioprocess and biosystems engineering

authors： Anaya-Reza O,Lopez-Arenas T

更新日期：2017-07-01 00:00:00

abstract：:The β-glucanase produced from Bacillus sp. CSB55 not only depicts the potent industrial characteristics but also relates as bio-industrial catalyst supporting the spontaneous formation of the products, high hydrolytic efficiency, and feasibility of the enzymatic reaction. A homogeneous β-glucanase (GluB55) was purifie...

journal_title：Bioprocess and biosystems engineering

authors： Regmi S,Choi YS,Kim YK,Khan MM,Lee SH,Choi YH,Cho SS,Jin YY,Yoo JC,Suh JW

更新日期：2020-02-01 00:00:00

abstract：:High amounts of outer membrane (OM) components were released in glucose-limited fed-batch (GLFB) cultures at 37 degrees C at specific growth rates approaching 0.05 h(-1). Endotoxin analyses from a 20 degrees C GLFB culture gave similar results. An alternative fermentation technique, the temperature-limited fed-batch (...

journal_title：Bioprocess and biosystems engineering

authors： Svensson M,Han L,Silfversparre G,Häggström L,Enfors SO

更新日期：2005-04-01 00:00:00

abstract：:Rice straw is a lignocellulosic biomass that constitutes a renewable organic substance and alternative source of energy; however, its structure confounds the liberation of monosaccharides. Pretreating rice straw using a TiO(2)/UV system facilitated its hydrolysis with Accellerase 1000(™), suggesting that hydroxyl radi...

journal_title：Bioprocess and biosystems engineering

authors： Kang HK,Kim D

更新日期：2012-01-01 00:00:00

abstract：:tert-Butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate [(3R,5S)-CDHH] is the key chiral intermediate to synthesize the side chain of the lipid-lowering drug rosuvastatin. Carbonyl reductases showed excellent activity for the biosynthesis of (3R,5S)-CDHH. The requirement of cofactor NADH/NADPH leads to high cost for the in...

journal_title：Bioprocess and biosystems engineering

authors： Zhang XJ,Wang WZ,Zhou R,Liu ZQ,Zheng YG

更新日期：2020-01-01 00:00:00

abstract：:Lactococcus lactis spp. lactis and Lactococcus lactis spp. cremoris are widely used in the manufacture of fermented milk. These strains were compared for production of Dipeptidyl Peptidase IV (DPP IV) enzyme in terms of enzyme activity, specific growth rates and productivity. Lactococcus lactis spp. lactis was produce...

journal_title：Bioprocess and biosystems engineering

authors： Ustün O,Ongen G

更新日期：2012-10-01 00:00:00

abstract：:Exogenous enzymes are extraneous enzymes that are not intrinsic to the subject. The exogenous enzyme industry has been rapidly developing recently. Successful application of recombinant DNA amplification, high-efficiency expression, and immobilization technology to genetically engineered bacteria provides a rich sourc...

journal_title：Bioprocess and biosystems engineering

authors： Yuan B,Zhou S,Liu C,Zhang S,Li J,Liu A

更新日期：2020-11-04 00:00:00

abstract：:Sucrose from sugarcane is produced in abundance in Brazil, which provides an opportunity to manufacture other high-value products. Gluconic acid (GA) can be produced by multi-enzyme conversion of sucrose using the enzymes invertase, glucose oxidase, and catalase. In this process, one of the byproducts is fructose, whi...

journal_title：Bioprocess and biosystems engineering

authors： Mafra AC,Furlan FF,Badino AC,Tardioli PW

更新日期：2015-04-01 00:00:00

abstract：:Erythromycin A is an important antibiotic. A chemically defined synthetic medium for erythromycin production was systematically optimized in this study. A high-throughput method was employed to reduce the number of components and optimize the concentration of each component. After two round single composition deletion...

journal_title：Bioprocess and biosystems engineering

authors： Hong M,Liao J,Chu J

更新日期：2018-10-01 00:00:00

abstract：:To obtain the soluble production of recombinant NovQ, it has been constructed into the pET28a system. Unfortunately, NovQ was mostly accumulated as inclusion bodies and existed in insoluble fractions of E. coli cell lysate. Four partners, namely His6, TrxA, GST and MBP, were investigated in fusion expression and co-ex...

journal_title：Bioprocess and biosystems engineering

authors： Ni W,Liu H,Wang P,Wang L,Sun X,Wang H,Zhao G,Zheng Z

更新日期：2019-03-01 00:00:00

abstract：:The paper describes a recombinant Schneider 2 (rS2) cell culture and protein expression in a bioreactor. S2 cells were transfected with a plasmid containing a fusion protein (human mu opioid receptor, hMOR, and green fluorescent protein, EGFP) under the control of inducible metallothionein promoter. A bioprocess in a ...

journal_title：Bioprocess and biosystems engineering

authors： Brillet K,da Conceição MM,Pattus F,Pereira CA

更新日期：2006-04-01 00:00:00

abstract：:A Candida rugosa lipase immobilized on polypropylene powder was employed as the biocatalyst for the enantioselective hydrolysis of (R, S)-suprofen 2,2,2-trifluorothioester in cyclohexane, in which trioctylamine was added as the catalyst to perform in situ racemization of the remaining (R)-thioester. A hollow-fiber mem...

journal_title：Bioprocess and biosystems engineering

authors： Wang LW,Cheng YC,Tsai SW

更新日期：2004-12-01 00:00:00

abstract：:Recently, several studies have been published on the application of plasmid DNA (pDNA) in gene therapy and vaccine production. The bioprocess to obtain pDNA involves the steps of fermentation, primary recovery, secondary recovery and final purification. The pDNA primary recovery, which is the key step to the rest of t...

journal_title：Bioprocess and biosystems engineering

authors： Padilla-Zamudio A,Guerrero-Germán P,Tejeda-Mansir A

更新日期：2015-06-01 00:00:00

abstract：:Harvesting technology has a significant influence on the microalgal biomass industry. This study develops a buoy-bead flotation method and analyzes the factors impacting flotation. Experimental results show that adding sodium borosilicate as an alternative microsphere material can result in 58.5% harvesting efficiency...

journal_title：Bioprocess and biosystems engineering

authors： Wen H,Zou X,Xu K,Shen Z,Ren X,Li Y

更新日期：2019-03-01 00:00:00

abstract：:Discontinuous airlift mixing was realized by injecting pressured air at time intervals with a frequency between 0.033 and 0.25 Hz (at 80 kPa; i.e., every 4-30 s; valve opening time 800 ms) into outdoor flat panel photobioreactors ([Formula: see text]). This caused a flow velocity between 2 and 20 cm s(-1) of the cultu...

journal_title：Bioprocess and biosystems engineering

authors： Leupold M,Hindersin S,Kerner M,Hanelt D

更新日期：2013-11-01 00:00:00