Abstract：
:An extractive biocatalytic method of aqueous two-phase system was employed for stereoinversing (R)-1-phenyl-1,2-ethanediol into (S)-1-phenyl-1,2-ethanediol by Candida parapsilosis CCTCC M203011. It was observed that substrate and product inhibitions in microbial stereoinversion through one-pot oxidation and reduction were removed efficiently by extractive biocatalysis in aqueous two-phase system with PEG 4000/phosphate potassium system, and that the substrate concentration was enhanced from 15 to 30 g/L with product optical purity of 99.02% e.e. and yield of 90% after 60 h. Simultaneously, it was observed that change in cell morphology impedes the further enhancement of substrate concentration in this system but can be reversibly changed after stereoinversion or cultivation in systems without PEG.
journal_name
Bioprocess Biosyst Engjournal_title
Bioprocess and biosystems engineeringauthors
Xu MW,Mu XQ,Xu Ydoi
10.1007/s00449-009-0334-9subject
Has Abstractpub_date
2010-03-01 00:00:00pages
367-73issue
3eissn
1615-7591issn
1615-7605journal_volume
33pub_type
杂志文章abstract：:A serine alkaline protease from a newly isolated alkaliphilic Bacillus altitudinis GVC11 was purified and characterized. The enzyme was purified to homogeneity by acetone precipitation, DEAE-cellulose anion exchange chromatography with 7.03-fold increase in specific activity and 15.25% recovery. The molecular weight o...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-010-0483-x
更新日期：2011-05-01 00:00:00
abstract：:ε-Poly-L-lysine (ε-PL), one of the only two homo-poly amino acids known in nature, is used as a preservative. In this study, strategies of feeding precursor L-lysine into 5 L laboratory scale fermenters, including optimization of L-lysine concentration and time, was investigated to optimize the production of ε-PL by S...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-013-0958-7
更新日期：2013-12-01 00:00:00
abstract：:High amounts of outer membrane (OM) components were released in glucose-limited fed-batch (GLFB) cultures at 37 degrees C at specific growth rates approaching 0.05 h(-1). Endotoxin analyses from a 20 degrees C GLFB culture gave similar results. An alternative fermentation technique, the temperature-limited fed-batch (...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-004-0377-x
更新日期：2005-04-01 00:00:00
abstract：:The fungicide carbendazim is an ecotoxic agent affecting aquatic biota. Due to its suspected hormone-disrupting effects, it is considered a "priority hazard substance" by the Water Framework Directive of the European Commission, and its degradation is of major concern. In this work, a horizontal tubular biofilm reacto...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-016-1717-3
更新日期：2017-04-01 00:00:00
abstract：:An new cascade control system is presented that reproducibly keeps the cultivation part of recombinant protein production processes on its predetermined track. While the system directly controls carbon dioxide production mass and carbon dioxide production rates along their setpoint profiles in fed-batch cultivation, i...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-011-0571-6
更新日期：2012-03-01 00:00:00
abstract：:In the present investigation, growth kinetics of Kocuria marina DAGII during batch production of β-Cryptoxanthin (β-CRX) was studied by considering the effect of glucose and maltose as a single and binary substrate. The importance of mixed substrate over single substrate has been emphasised in the present study. Diffe...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-016-1678-6
更新日期：2017-01-01 00:00:00
abstract：:Light and bacteria can be used in combination to enhance secondary metabolite production during fermentation. Red yeast rice powder (RYRP) was inoculated with Bacillus subtilis (B2) isolated from freshwater seafood and incubated under light-emitting diodes (LEDs) of different colors (blue, green, red, white), fluoresc...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-018-2056-3
更新日期：2019-04-01 00:00:00
abstract：:tert-Butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate [(3R,5S)-CDHH] is the key chiral intermediate to synthesize the side chain of the lipid-lowering drug rosuvastatin. Carbonyl reductases showed excellent activity for the biosynthesis of (3R,5S)-CDHH. The requirement of cofactor NADH/NADPH leads to high cost for the in...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-019-02201-x
更新日期：2020-01-01 00:00:00
abstract：:UV photolysis was used to relieve inhibition of biomass growth by sulfadiazine (SD), a broad-spectrum anti-microbial. To investigate the effects of SD on biomass growth, three substrates-glucose alone (G), glucose plus sulfadiazine (G+SD), and glucose plus photolyzed SD (G+PSD)-were used to culture the bacteria acclim...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-014-1335-x
更新日期：2015-05-01 00:00:00
abstract：:The β-glucanase produced from Bacillus sp. CSB55 not only depicts the potent industrial characteristics but also relates as bio-industrial catalyst supporting the spontaneous formation of the products, high hydrolytic efficiency, and feasibility of the enzymatic reaction. A homogeneous β-glucanase (GluB55) was purifie...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-019-02221-7
更新日期：2020-02-01 00:00:00
abstract：:A two-phase dispersion technique, termed emulsification-internal gelation, is proposed for encapsulation of activated sludge in gellan gum microbeads. The influence of emulsion parameters on size distribution of microbeads was investigated. Mean diameter of microbeads varied within a range of 34-265 microm as a descen...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-004-0360-6
更新日期：2004-07-01 00:00:00
abstract：:The aim of this study is to investigate the effect of the N/P ratio on biomass growth with the simultaneous removal of nutrients from municipal wastewaters. An optical panel photobioreactor is employed for this investigation because it provides a uniform light distribution within the reactor, which enhances the effici...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-014-1317-z
更新日期：2015-04-01 00:00:00
abstract：:The aim of this study is to achieve high-level extracellular production of D-Psicose-3-epimerase (DPE) with recombinant Escherichia coli. High-level production of DPE is one of the key factors in D-Psicose production. In the present study, the gene AAL45544.1 from Agrobacterium tumefaciens str. C58 was modified by art...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-013-0952-0
更新日期：2013-11-01 00:00:00
abstract：:An alternating pumped sequencing batch biofilm reactor (APSBBR) system was developed to treat small-scale domestic wastewater. This laboratory system had two reactor tanks, Reactor 1 and Reactor 2, with two identical plastic biofilm modules in each reactor. Reactor 1 of the APSBBR had five operational phases--fill, an...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-005-0038-8
更新日期：2006-04-01 00:00:00
abstract：:Corrosion in bioprocessing applications is described for a 25-year-old bioprocessing pilot plant facility. Various available stainless steel alloys differ greatly in properties owing to the impact of specific alloying elements and their concentrations. The alloy property evaluated was corrosion resistance as a functio...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章,评审
doi：10.1007/s00449-008-0223-7
更新日期：2009-01-01 00:00:00
abstract：:This work proposes a committee of Cascade Correlation neural networks as an online smoother for random measurement noise. The goals of this paper are twofold: first it intends to explore the possibilities of using a constructive neural network algorithm to learn how to filter typical noisy data from a bioreactor, CO(2...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-007-0152-x
更新日期：2008-02-01 00:00:00
abstract：:Armillaria sp. F022 is a white-rot fungus isolated from a tropical rain forest in Indonesia that is capable of utilizing pyrene as a source of carbon and energy. Enzymes production during the degradation process by Armillaria sp. F022 was certainly related to the increase in biomass. In the first week after incubation...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-012-0803-4
更新日期：2013-04-01 00:00:00
abstract：:This study assessed the simultaneous nitrification and denitrification processes and remaining organic matter removal from anaerobic reactor effluent treating wastewater in a single reactor. A structured-bed reactor, with polyurethane foam as support media, was subjected to intermittent aeration and effluent recircula...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-018-1940-1
更新日期：2018-08-01 00:00:00
abstract：:The effect of several solvents present in a biphasic reaction system on cells of Rhodococcus erythropolis DCL14, Xanthobacter Py2, Arthrobacter simplex and Mycobacterium sp. NRRL B-3805 was evaluated. These four strains have been widely exploited, from bioremediation to the production of fine chemicals, in two-phase r...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-004-0381-1
更新日期：2004-12-01 00:00:00
abstract：:In this study, the microalga Chlorella saccharophila was subjected to ultraviolet (UV) mutagenesis, and mutant screening was conducted based on acidity tolerance to generate mutants with increased triacylglycerol (TAG) and polyunsaturated fatty acid (PUFA) contents. Two improved mutant strains (M1 and M5) were generat...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-019-02153-2
更新日期：2019-10-01 00:00:00
abstract：:Bacterial cellulose (BC) exhibits unique properties such as high purity compared to plant-based cellulose; however, commercial production of BC has remained a challenge, primarily due to the strain properties of cellulose-producing bacteria. Herein, we developed a functional and stable BC production system in genetica...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-017-1864-1
更新日期：2018-02-01 00:00:00
abstract：:A highly efficient β-1,4-mannanase-secreting strain, Pholiota adiposa SKU0714, was isolated and identified on the basis of its morphological features and sequence analysis of internal transcribed spacer rDNA. P. adiposa β-1,4-mannanase was purified to homogeneity from P. adiposa culture supernatants by one-step chroma...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-014-1156-y
更新日期：2014-09-01 00:00:00
abstract：:Ginsenoside compound K (CK) with a wide range of pharmacological activities has been widely used in the healthcare product industry. However, the application of CK is limited by low productivity and difficult separation. The purpose of this study is to convert ginsenoside Rb1 into CK by improving conversion efficiency...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-020-02314-8
更新日期：2020-07-01 00:00:00
abstract：:A CO2-added ammonia explosion pretreatment was performed for bioethanol production from rice straw. The pretreatment conditions, such as ammonia concentration, CO2 loading level, residence time, and temperature were optimized using response surface methodology. The response for optimization was defined as the glucose ...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-014-1165-x
更新日期：2014-09-01 00:00:00
abstract：:Laccases are very interesting biocatalysts of recognized importance for several industrial applications. Its production by Trametes versicolor, a white-rot fungus, was induced by a combination of cotton gin wastes (1%), a lignocellulosic waste, and vinasse (15%), an industrial by-product from sugarcane industry. The u...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-019-02245-z
更新日期：2020-03-01 00:00:00
abstract：:Gamma-aminobutyric acid (GABA) production by free and Ca-alginate encapsulated cells of Enterococcus faecium CFR 3003 was investigated. Mass transfer rates characterizing the GABA production process using encapsulated cells were investigated. Experiments were performed to investigate external film and internal pore di...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-014-1296-0
更新日期：2015-03-01 00:00:00
abstract：:Unfolded protein response (UPR) usually happens when expressing heterologous proteins in high level, which may help cells to facilitate protein processing. Here, we evaluated the effects of the UPR activator HAC1p on a raw-starch hydrolyzing α-amylase (Gs4j-amyA), so as to improve heterologous production of the enzyme...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-016-1701-y
更新日期：2017-03-01 00:00:00
abstract：:Tissue engineered constructs reported to date have been prepared primarily from poly(glycolic) acid or collagen scaffolds onto which cells are grown and matured. In this paper we report experimental data to demonstrate the use of a natural, human protein, as a tubular scaffold for vascular grafting. Using a manual and...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s004490100268
更新日期：2002-04-01 00:00:00
abstract：:Biochemical reactions occurring during anaerobic digestion have been modelled using reaction kinetic equations such as first-order, Contois and Monod which are then combined to form mechanistic models. This work considers models which include between one and three biochemical reactions to investigate if the choice of ...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-016-1577-x
更新日期：2016-06-01 00:00:00
abstract：:The phasing technique is a method for synchronizing cell populations in a bioreactor. Periodic changes of substrate supply and depletion can provoke a cell cycle phasing of originally stochastic scattered proliferation patterns. Synchronized cell populations characterized by changes in DNA content distribution can be ...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-007-0180-6
更新日期：2008-08-01 00:00:00
