Measuring the adhesion limit of fibronectin for fibroblasts with a narrow-gap rotational rheometer.

abstract：:Amano lipase AK from P. fluorescens was immobilized on different types of chitosan-containing supports. Chitosan lower molecular weight (2.5%), chitosan lower molecular weight/sodium alginate (2.5%/2.5%) and chitosan lower molecular weight/carrageenan (2.5%/2.5%) allowed the highest values of immobilization yields (IY...

journal_title：Bioprocess and biosystems engineering

authors： de Sousa Fonseca T,de Oliveira UMF,de Oliveira MDCF,de Lemos TLG,da Silva MR,Rios NS,Gonçalves LRB,de Mattos MC

更新日期：2021-01-03 00:00:00

abstract：:Industrial grade soluble corn starch was used directly and effectively as the fermentation substrate for microbial exopolysaccharides production. Bacillus subtilis mutant strain NJ308 grew with untreated starch raw material as the sole carbon source. The real-time PCR results demonstrated that up-regulated genes encod...

journal_title：Bioprocess and biosystems engineering

authors： He X,He F,Hang J,Li H,Chen Y,Wei P,Chen K,Li Y,OuYang P

更新日期：2018-06-01 00:00:00

abstract：:(R)-2-hydroxy-4-phenylbutyric acid (R-HPBA) is a valuable intermediate for the synthesis of angiotensin-converting enzyme inhibitors. The asymmetric reduction of 2-oxo-4-phenylbutyric acid (OPBA) by oxidoreductases is an efficient approach for its synthesis. Here, we report a novel biocatalytic approach for asymmetric...

journal_title：Bioprocess and biosystems engineering

authors： Wang X,Yu Z,Tang J,Yi D,Chen S

更新日期：2018-09-01 00:00:00

abstract：:Lysozyme, a type of natural enzyme, has been widely used for bacteriostatic functionalization of various materials due to its efficient and selective antibacterial properties. Herein, we report the preparation and characterization of an eco-friendly antibacterial textile based on the immobilization of lysozyme from ch...

journal_title：Bioprocess and biosystems engineering

authors： Yang W,Zhang N,Wang Q,Wang P,Yu Y

更新日期：2020-09-01 00:00:00

abstract：:Chinese hamster ovary (CHO) cells are the main workhorse in the biopharmaceutical industry for the production of recombinant proteins, such as monoclonal antibodies. To date, a variety of metabolic engineering approaches have been used to improve the productivity of CHO cells. While genetic manipulations are potential...

journal_title：Bioprocess and biosystems engineering

authors： Fouladiha H,Marashi SA,Torkashvand F,Mahboudi F,Lewis NE,Vaziri B

更新日期：2020-08-01 00:00:00

abstract：:Long-term and excessive use of monocrotophos (MPs) pesticide leads to an accumulation of MPs residues in agricultural products. Electrochemical biosensor technology was developed as a simple and efficient method for detecting MPs. However, commercial acetylcholinesterase (AChE) sensors are not applied in practical MPs...

journal_title：Bioprocess and biosystems engineering

authors： Jiaojiao X,Bin Z,Pengyun W,Qing L,Xin S,Rui J

更新日期：2020-02-01 00:00:00

abstract：:ε-Poly-L-lysine (ε-PL), one of the only two homo-poly amino acids known in nature, is used as a preservative. In this study, strategies of feeding precursor L-lysine into 5 L laboratory scale fermenters, including optimization of L-lysine concentration and time, was investigated to optimize the production of ε-PL by S...

journal_title：Bioprocess and biosystems engineering

authors： Chen XS,Ren XD,Zeng X,Zhao FL,Tang L,Zhang HJ,Zhang JH,Mao ZG

更新日期：2013-12-01 00:00:00

abstract：:In this study, Escherichia coli cells overexpressing lysine decarboxylase (CadA) were used for cadaverine production. Barium alginate was selected as a matrix for immobilization of E. coli YH91. Free cells and immobilized cells (IC) were characterized for their physiochemical properties, and the optimum pH and tempera...

journal_title：Bioprocess and biosystems engineering

authors： Bhatia SK,Kim YH,Kim HJ,Seo HM,Kim JH,Song HS,Sathiyanarayanan G,Park SH,Park K,Yang YH

更新日期：2015-12-01 00:00:00

abstract：:Fibrous poly(styrene-b-glycidylmethacrylate) brushes were grafted on poly(styrene-divinylbenzene) (P(S-DVB)) beads using surface-initiated atom transfer radical polymerization. Tetraethyldiethylenetriamine (TEDETA) ligand was incorporated on P(GMA) block. The ligand attached beads were used for reversible immobilizati...

journal_title：Bioprocess and biosystems engineering

authors： Bayramoglu G,Karagoz B,Altintas B,Arica MY,Bicak N

更新日期：2011-08-01 00:00:00

abstract：:The effect of several solvents present in a biphasic reaction system on cells of Rhodococcus erythropolis DCL14, Xanthobacter Py2, Arthrobacter simplex and Mycobacterium sp. NRRL B-3805 was evaluated. These four strains have been widely exploited, from bioremediation to the production of fine chemicals, in two-phase r...

journal_title：Bioprocess and biosystems engineering

authors： de Carvalho CC,da Fonseca MM

更新日期：2004-12-01 00:00:00

abstract：:In order to control suitable mycelium morphology to obtain high lipase productivity by Rhizopus chinensis in submerged fermentation, the effects of fungal morphology on the lipase production by this strain both in shake flask and fermentor were investigated. Different inoculum level and shear stress were used to devel...

journal_title：Bioprocess and biosystems engineering

authors： Teng Y,Xu Y,Wang D

更新日期：2009-04-01 00:00:00

abstract：:Pullulanase was extracellularly produced with an engineered Escherichia coli with a combined strategy. When auto-induction instead of isopropyl β-D-1-thiogalactopyranoside (IPTG) induction method was implemented, we observed increased extracellular activity (4.2 U ml(-1)) and cell biomass (7.95 g DCW l(-1)). Subsequen...

journal_title：Bioprocess and biosystems engineering

authors： Chen WB,Nie Y,Xu Y,Xiao R

更新日期：2014-04-01 00:00:00

abstract：:A strain designated M866, producing kojic acid with a high yield, was obtained by combining induced mutation using ion beam implantation and ethyl methane sulfonate treatment of a wild type strain of Aspergillus oryzae B008. The amount of kojic acid produced by the strain M866 in a shaking flask was 40.2 g/L from 100 ...

journal_title：Bioprocess and biosystems engineering

authors： Yan S,Tang H,Wang S,Xu L,Liu H,Guo Y,Yao J

更新日期：2014-06-01 00:00:00

abstract：:Estimation of the energy capacity of a microbial cell mass on the basis of its lipid content and elemental composition can be used for the comparative evaluation of different microbial sources of biodiesel. Lipomyces starkeyi cell mass concentration reached 94.6 g/L with 37.4% of lipids in a fed-batch process using xy...

journal_title：Bioprocess and biosystems engineering

authors： Anschau A,Franco TT

更新日期：2015-08-01 00:00:00

abstract：:An environmentally friendly chemical, tetrakis(hydroxymethyl)phosphonium sulfate (THPS), was used as a metabolic uncoupler to reduce sludge production in a pilot-scale anaerobic/anoxic/oxic process. The results show that the addition of THPS (1.08-1.86 mL/m(3) influent) in the sludge return section could reduce waste ...

journal_title：Bioprocess and biosystems engineering

authors： Guo X,Yang J,Liang Y,Liu J,Xiao B

更新日期：2014-03-01 00:00:00

abstract：:Biodiesel production from microalgae depends on the biomass concentration and lipid content in microalgal cells. Photobioreactors (PBRs) facilitates cultivation of microalgae and renders better process control than open systems. However, reactor configuration and consequential hydrodynamics considerably influence biom...

journal_title：Bioprocess and biosystems engineering

authors： Banerjee S,Dasgupta S,Das D,Atta A

更新日期：2020-08-01 00:00:00

abstract：:The stereoselective three-enzyme cascade for the one-pot synthesis of (1S,2S)-1-phenylpropane-1,2-diol ((1S,2S)-1-PPD) from inexpensive starting substrates, benzaldehyde and acetaldehyde, was explored. By coupling stereoselective carboligation catalyzed by benzoylformate decarboxylase (BFD), L-selective reduction of a...

journal_title：Bioprocess and biosystems engineering

authors： Presečki AV,Pintarić L,Švarc A,Vasić-Rački Đ

更新日期：2018-06-01 00:00:00

abstract：:Bio-catalytic in vitro multistep reactions can be combined in a single step in one pot by optimizing multistep reactions under identical reaction condition. Using this analogy, the process of making PEGylated insulin, IN-105, was simplified. Instead of taking the purified active insulin bulk powder as the starting mat...

journal_title：Bioprocess and biosystems engineering

authors： Hazra P,Chatterjee A,Shabandri Q,Adhikary L,Dave N,Buddha M

更新日期：2012-10-01 00:00:00

abstract：:Kaolin showed as a very perspective carrier for the enzyme immobilization and it was used for the adsorption of horseradish peroxidase (HRP). The effects of the enzyme concentration and pH on the immobilization efficiency were studied in the reaction with pyrogallol and anthraquinone dye C.I. Acid Violet 109 (AV 109)....

journal_title：Bioprocess and biosystems engineering

authors： Šekuljica NŽ,Prlainović NŽ,Jovanović JR,Stefanović AB,Djokić VR,Mijin DŽ,Knežević-Jugović ZD

更新日期：2016-03-01 00:00:00

abstract：:The mammalian pathogen Bordetella bronchiseptica was grown under controlled batch conditions with glutamate as the primary carbon and nitrogen source. First, a Box-Behnken statistical design quantified the effect of Mg, sulfate, and nicotinate on the antigen filamentous hemagglutinin (FHA) formation. Using lactic acid...

journal_title：Bioprocess and biosystems engineering

authors： Guetter SD,Eiteman MA

更新日期：2014-02-01 00:00:00

abstract：:Unfolded protein response (UPR) usually happens when expressing heterologous proteins in high level, which may help cells to facilitate protein processing. Here, we evaluated the effects of the UPR activator HAC1p on a raw-starch hydrolyzing α-amylase (Gs4j-amyA), so as to improve heterologous production of the enzyme...

journal_title：Bioprocess and biosystems engineering

authors： Huang M,Gao Y,Zhou X,Zhang Y,Cai M

更新日期：2017-03-01 00:00:00

abstract：:The production of chitinases and hydrophobins from Lecanicillium lecanii was influenced by the cultivation method and type of carbon source. Crude enzyme obtained from solid-substrate culture presented activities of exochitinases (32 and 51 kDa), endochitinases (26 kDa), β-N-acetylhexosaminidases (61, 80, 96 and 111 k...

journal_title：Bioprocess and biosystems engineering

authors： Rocha-Pino Z,Vigueras G,Shirai K

更新日期：2011-08-01 00:00:00

abstract：:Twenty-nine actinobacterial strains were isolated from marine sponge Spongia officinalis and screened for antagonistic activity against various bacterial and fungal pathogens. The active antibiotic producer MAPS15 was identified as Streptomyces sp. using 16S rRNA phylogenetic analysis. The critical control factors wer...

journal_title：Bioprocess and biosystems engineering

authors： Sathiyanarayanan G,Gandhimathi R,Sabarathnam B,Seghal Kiran G,Selvin J

更新日期：2014-03-01 00:00:00

abstract：:This paper presents a methodology for the determination of reaction rate constants for nitrifying bacteria and their mean population percentage in biomass in an alternating oxidation ditch system. The method used is based on the growth rate equations of the ASM1 model (IWA) (Henze et al. in Activated sludge models ASM...

journal_title：Bioprocess and biosystems engineering

authors： Mantziaras ID,Katsiri A

更新日期：2011-01-01 00:00:00

abstract：:Broad-spectrum antibodies can effectively recognize substances with similar structures and have broad application prospects in field rapid detection. In this study, broad-spectrum antibodies (Abs) against organophosphorus pesticides (OPs) were used as sensitive recognition elements, which could effectively recognize m...

journal_title：Bioprocess and biosystems engineering

authors： Dong H,Zhao Q,Li J,Xiang Y,Liu H,Guo Y,Yang Q,Sun X

更新日期：2020-11-08 00:00:00

abstract：:Laccases are very interesting biocatalysts of recognized importance for several industrial applications. Its production by Trametes versicolor, a white-rot fungus, was induced by a combination of cotton gin wastes (1%), a lignocellulosic waste, and vinasse (15%), an industrial by-product from sugarcane industry. The u...

journal_title：Bioprocess and biosystems engineering

authors： Pinheiro VE,Michelin M,Vici AC,de Almeida PZ,Teixeira de Moraes Polizeli ML

更新日期：2020-03-01 00:00:00

abstract：:Erythromycin A is an important antibiotic. A chemically defined synthetic medium for erythromycin production was systematically optimized in this study. A high-throughput method was employed to reduce the number of components and optimize the concentration of each component. After two round single composition deletion...

journal_title：Bioprocess and biosystems engineering

authors： Hong M,Liao J,Chu J

更新日期：2018-10-01 00:00:00

abstract：:UV photolysis was used to relieve inhibition of biomass growth by sulfadiazine (SD), a broad-spectrum anti-microbial. To investigate the effects of SD on biomass growth, three substrates-glucose alone (G), glucose plus sulfadiazine (G+SD), and glucose plus photolyzed SD (G+PSD)-were used to culture the bacteria acclim...

journal_title：Bioprocess and biosystems engineering

authors： Pan S,Yan N,Zhang Y,Rittmann BE

更新日期：2015-05-01 00:00:00

abstract：:High amounts of outer membrane (OM) components were released in glucose-limited fed-batch (GLFB) cultures at 37 degrees C at specific growth rates approaching 0.05 h(-1). Endotoxin analyses from a 20 degrees C GLFB culture gave similar results. An alternative fermentation technique, the temperature-limited fed-batch (...

journal_title：Bioprocess and biosystems engineering

authors： Svensson M,Han L,Silfversparre G,Häggström L,Enfors SO

更新日期：2005-04-01 00:00:00

abstract：:Fluxes of central carbon metabolism [glycolysis, pentose phosphate pathway (PPP), tricarboxylic acid cycle (TCA cycle), biomass formation] were determined for several Bacillus megaterium strains (DSM319, WH320, WH323, MS941) in C- and N-limited chemostat cultures by (13)C labelling experiments. The labelling patterns ...

journal_title：Bioprocess and biosystems engineering

authors： Fürch T,Hollmann R,Wittmann C,Wang W,Deckwer WD

更新日期：2007-01-01 00:00:00