Abstract：
:In the present study, it was presented a strategy to maximize the cutinase production by solid-state fermentation from different microorganisms and substrates. The best results were observed using Fusarium verticillioides, rice bran being the main substrate. Maximum yield of cutinase obtained by the strain was 16.22 U/g. For concentration, ethanol precipitation was used, and the purification factor was 2.4. The optimum temperature and pH for enzyme activity were 35 °C and 6.5, respectively. The enzyme was stable at a wide range of temperature and at all pH values tested. The concentrated cutinase was used as an adjuvant in a formulation containing cutinase + bioherbicide. The use of enzyme increased the efficiency of bioherbicide, since cutinase was responsible to remove/degrade the cutin that recovery the weed leaves and difficult the bioherbicide absorption. Cutinase showed to be a promising product to be used in formulation of bioherbicides.
journal_name
Bioprocess Biosyst Engjournal_title
Bioprocess and biosystems engineeringauthors
de Oliveira CT,Alves EA,Todero I,Kuhn RC,de Oliveira D,Mazutti MAdoi
10.1007/s00449-019-02086-wsubject
Has Abstractpub_date
2019-05-01 00:00:00pages
829-838issue
5eissn
1615-7591issn
1615-7605pii
10.1007/s00449-019-02086-wjournal_volume
42pub_type
杂志文章abstract：:The effects of light, pH and organic carbon sources were investigated on hydrogen production by algae. An optical fiber was examined as an internal light source. The optical fiber rendered prolonged lag time and total time of hydrogen production. The optimal pH to produce hydrogen for Chlorella sp. was 8.0. Glucose, f...
journal_title：Bioprocess and biosystems engineering
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journal_title：Bioprocess and biosystems engineering
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