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Membrane fluidity of halophilic ectoine-secreting bacteria related to osmotic and thermal treatment.

Abstract：

:In response to sudden decrease in osmotic pressure, halophilic microorganisms secrete their accumulated osmolytes. This specific stress response, combined with physiochemical responses to the altered environment, influence the membrane properties and integrity of cells, with consequent effects on growth and yields in bioprocesses, such as bacterial milking. The aim of this study was to investigate changes in membrane fluidity and integrity induced by environmental stress in ectoine-secreting organisms. The halophilic ectoine-producing strains Alkalibacillus haloalkaliphilus and Chromohalobacter salexigens were treated hypo- and hyper-osmotically at several temperatures. The steady-state anisotropy of fluorescently labeled cells was measured, and membrane integrity assessed by flow cytometry and ectoine distribution. Strong osmotic downshocks slightly increased the fluidity of the bacterial membranes. As the temperature increased, the increasing membrane fluidity encouraged more ectoine release under the same osmotic shock conditions. On the other hand, combined shock treatments increased the number of disintegrated cells. From the ectoine release and membrane integrity measurements under coupled thermal and osmotic shock conditions, we could optimize the secretion conditions for both bacteria.

journal_name

Bioprocess Biosyst Eng

journal_title

Bioprocess and biosystems engineering

authors

Bergmann S,David F,Clark W,Wittmann C,Krull R

doi

10.1007/s00449-013-0957-8

subject

Has Abstract

pub_date

2013-12-01 00:00:00

pages

1829-41

issue

12

eissn

1615-7591

issn

1615-7605

journal_volume

36

pub_type

杂志文章

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