Abstract:
:Porphyridium cruentum is one of the most valued microalgae species able to produce both pigments and exopolysaccharides. Conventional liquid suspended cultivation in ponds and photobioreactors show its disadvantages in lower cultivation efficiency and higher stirring power consumption due to the high viscosity of the medium by the accumulation of polysaccharides. In this work, a new method of culture (called attached cultivation) based on the growth of microalgae using a supporting surface was successfully applied to the cultivation of P. cruentum and the effect of the main influential parameters on its growth rate and polysaccharides production has been investigated. Higher values of these factors resulted in a faster growth rate and, in particular, optimum values of 6.98 g m-2 for initial biomass density, 100 µmol m-2 s-1 for light intensity, continuous illumination, 2.0 % for CO2 concentration, and 0.1 v v-1 min-1 for aeration rate produced the best polysaccharide production of 42 % dry weight. The nutrition profile of P. cruentum obtained in attached and suspended cultivations was similar. Overall these results demonstrate that the attached cultivation is a promising technique which greatly improves the growth rate of P. cruentum as well as its production of polysaccharides and, therefore, it is worth enhancing to be exploited for commercial application.
journal_name
Bioprocess Biosyst Engjournal_title
Bioprocess and biosystems engineeringauthors
Lutzu GA,Zhang L,Zhang Z,Liu Tdoi
10.1007/s00449-016-1676-8subject
Has Abstractpub_date
2017-01-01 00:00:00pages
73-83issue
1eissn
1615-7591issn
1615-7605pii
10.1007/s00449-016-1676-8journal_volume
40pub_type
杂志文章abstract::In the present investigation, growth kinetics of Kocuria marina DAGII during batch production of β-Cryptoxanthin (β-CRX) was studied by considering the effect of glucose and maltose as a single and binary substrate. The importance of mixed substrate over single substrate has been emphasised in the present study. Diffe...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-016-1678-6
更新日期:2017-01-01 00:00:00
abstract::This work aimed to develop an optimal carbon source feeding strategy to achieve maximal production of heparosan as a precursor of bioengineered heparin by Escherichia coli K5. Glycerol gave higher heparosan titer and productivity compared to glucose. The maximum heparosan production (187 mg/L) and heparosan productivi...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-012-0708-2
更新日期:2012-09-01 00:00:00
abstract::Fibrous poly(styrene-b-glycidylmethacrylate) brushes were grafted on poly(styrene-divinylbenzene) (P(S-DVB)) beads using surface-initiated atom transfer radical polymerization. Tetraethyldiethylenetriamine (TEDETA) ligand was incorporated on P(GMA) block. The ligand attached beads were used for reversible immobilizati...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-011-0523-1
更新日期:2011-08-01 00:00:00
abstract::Exogenous enzymes are extraneous enzymes that are not intrinsic to the subject. The exogenous enzyme industry has been rapidly developing recently. Successful application of recombinant DNA amplification, high-efficiency expression, and immobilization technology to genetically engineered bacteria provides a rich sourc...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章,评审
doi:10.1007/s00449-020-02463-w
更新日期:2020-11-04 00:00:00
abstract::During the production of bio-based bulk chemicals, such as lactic acid (LA), organic impurities have to be removed to produce a ready-to-market product. A capillary electrophoresis method for the simultaneous detection of LA and organic impurities in less than 10 min was developed. LA and organic impurities were detec...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-017-1761-7
更新日期:2017-07-01 00:00:00
abstract::We generated a genetically engineered Klebsiella pneumoniae strain (AK-VOT) to eliminate by-product formation during production of 1,3-propanediol (1,3-PD) from glycerol. In the present study, the glycerol-metabolizing properties of the recombinant strain were examined during fermentation in a 5 L bioreactor. As expec...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-011-0603-2
更新日期:2012-01-01 00:00:00
abstract::Determination of hydrogen peroxide (H2O2) has become essential in pharmaceutical, biological, clinical and environmental studies. The conventional detection methods of H2O2 such as colourimetry, titration, chromatography, spectrophotometry, fluorimetry, chemiluminescence have limited success, due to their poor selecti...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章,评审
doi:10.1007/s00449-017-1878-8
更新日期:2018-03-01 00:00:00
abstract::In this study, a newly isolated ascomycete fungus Trichoderma lixii F21 was explored to bioremediate the polar [Alizarin Red S (ARS)] and non-polar [Quinizarine Green SS (QGSS)] anthraquinone dyes. The bioremediation of ARS and QGSS by T. lixii F21 was found to be 77.78 and 98.31 %, respectively, via biosorption and e...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-016-1677-7
更新日期:2017-01-01 00:00:00
abstract::A Candida rugosa lipase immobilized on polypropylene powder was employed as the biocatalyst for the enantioselective hydrolysis of (R, S)-suprofen 2,2,2-trifluorothioester in cyclohexane, in which trioctylamine was added as the catalyst to perform in situ racemization of the remaining (R)-thioester. A hollow-fiber mem...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-004-0379-8
更新日期:2004-12-01 00:00:00
abstract::Candida cylindracea NRRL Y-17506 was grown to produce extracellular lipase from oleic acid as a carbon source. Through flask cultures, it was found that the optimum initial oleic acid concentration for cell growth was 20 g l(-1). However, high initial concentrations of oleic acid up to 50 g l(-1) were not inhibitory. ...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-006-0058-z
更新日期:2006-06-01 00:00:00
abstract::This study aimed to assess the variability in respect of titer and properties of xylanase from Trichoderma reesei SAF3 under both solid-state and submerged fermentation. SSF was initially optimized with different agro-residues and among them wheat bran was found to be the best substrate that favored maximum xylanase p...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-012-0761-x
更新日期:2013-01-01 00:00:00
abstract::A carbon membrane-aerated biofilm reactor (CMABR) was developed to treat synthetic wastewater. Such membrane exhibited a high degree of adhesion and good permeability. Continuous experiments showed that COD and NH4(+)-N removal efficiency were 90 +/- 2 and 92 +/- 4% at removal rates of 35.6 +/- 3.8 g COD/m2 per day an...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-007-0116-1
更新日期:2007-07-01 00:00:00
abstract::In this study, sodium gluconate was applied as a novel carbon source for the fuel ethanol production using an engineered Escherichia coli strain KO11 in batch fermentations. Ethanol and acetic acid were produced as two major products as well as small amount of lactic acid during the fermentation. Compared to the conve...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-013-0938-y
更新日期:2013-11-01 00:00:00
abstract::Enzymatic modification of starch using long chain fatty acid makes it thermoplastic suitable for a myriad of industrial applications. An industrial lipase preparation produced by Burkholderia cepacia (lipase PS) was used for modification of cassava starch with two acyl donors, lauric acid and palmitic acid. Reactions ...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-006-0060-5
更新日期:2006-06-01 00:00:00
abstract::The discovery of quorum sensing as a mechanism for regulating specific phenotypes in bacteria based on population density has conveyed attention to find molecules capable of interfering quorum sensing networks (QSN) in a process coined quorum quenching. Here, we examined the dynamics of Escherichia coli AI-2 and Pseud...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-008-0276-7
更新日期:2009-06-01 00:00:00
abstract::A highly efficient β-1,4-mannanase-secreting strain, Pholiota adiposa SKU0714, was isolated and identified on the basis of its morphological features and sequence analysis of internal transcribed spacer rDNA. P. adiposa β-1,4-mannanase was purified to homogeneity from P. adiposa culture supernatants by one-step chroma...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-014-1156-y
更新日期:2014-09-01 00:00:00
abstract::In this study, aiming to improve the economic feasibility of acetone-butanol-ethanol (ABE) fermentation process, generate valuable products and extend the product chain, esterification catalyzed by Candida sp. 99-125 cells was hybrid with the ABE fermentation-gas-stripping integration system. The gas-stripping condens...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-018-1960-x
更新日期:2018-09-01 00:00:00
abstract::The effluent from the biogas process was tested as a nutrient source during cultivation of the protein-rich and edible microalgae Spirulina (Arthrospira platensis) and compared with conventional Spirulina medium. Equal biomass production was observed until late exponential phase and no significant differences could be...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-016-1726-2
更新日期:2017-04-01 00:00:00
abstract::Complementary DNA (cDNA) encoding the new versatile peroxidase from the ligninolytic basidiomycete Pleurotus eryngii has been expressed in the ascomycete Emericella nidulans. In recombinant E. nidulans cultures, the pH reached values as high as 8.3, correlating with a sharp decrease in peroxidase activity. Peroxidase ...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-004-0365-1
更新日期:2004-10-01 00:00:00
abstract::(R)-2-hydroxy-4-phenylbutyric acid (R-HPBA) is a valuable intermediate for the synthesis of angiotensin-converting enzyme inhibitors. The asymmetric reduction of 2-oxo-4-phenylbutyric acid (OPBA) by oxidoreductases is an efficient approach for its synthesis. Here, we report a novel biocatalytic approach for asymmetric...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-018-1965-5
更新日期:2018-09-01 00:00:00
abstract::Several carbon sources were investigated for the production of 1,3-propanediol (PDO) and 2,3-butanediol (BDO) simultaneously, using an isolated indigenous Klebsiella sp. Ana-WS5. The results indicate that glycerol is a suitable carbon source for both BDO and PDO production. Further investigation suggests that adjustme...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-013-1008-1
更新日期:2014-03-01 00:00:00
abstract::A pilot-scale ultrafiltration membrane bioreactor (MBR) was used for the aerobic treatment of urban wastewater in four experimental stages influenced by seasonal temperature and different sets of operation conditions. The structure of the ammonia-oxidizing bacteria (AOB) community was profiled by temperature gradient ...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-012-0823-0
更新日期:2013-07-01 00:00:00
abstract::tert-Butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate [(3R,5S)-CDHH] is the key chiral intermediate to synthesize the side chain of the lipid-lowering drug rosuvastatin. Carbonyl reductases showed excellent activity for the biosynthesis of (3R,5S)-CDHH. The requirement of cofactor NADH/NADPH leads to high cost for the in...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-019-02201-x
更新日期:2020-01-01 00:00:00
abstract::The gene dehalA encoding a novel dichloromethane dehalogenases (DehalA), has been cloned from Bacillus circulans WZ-12 CCTCC M 207006. The open reading frame of dehalA, spanning 864 bp, encoded a 288-amino acid protein that showed 85.76% identity to the dichloromethane dehalogenases of Hyphomicrobium sp. GJ21 with sev...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-009-0311-3
更新日期:2009-10-01 00:00:00
abstract::Staphylococcal enterotoxin B (SEB) is one of many toxins produced by the Gram-positive bacterium Staphylococcal aureus. While SEB is known as the causative agent of certain food poisonings it is also considered abiological select agent. Thus, rapid and accurate identification of SEB during either surveillance or in re...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-007-0168-2
更新日期:2008-06-01 00:00:00
abstract::To improve the performance of covalently immobilized penicillin acylase (PA), the immobilization was carried out in mesocellular silica foams (MCFs) using p-benzoquinone as cross linker. The characterizations of the immobilized enzyme were studied carefully. The results showed that the relative activity of the immobil...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-007-0189-x
更新日期:2008-08-01 00:00:00
abstract::Gamma-aminobutyric acid (GABA) production by free and Ca-alginate encapsulated cells of Enterococcus faecium CFR 3003 was investigated. Mass transfer rates characterizing the GABA production process using encapsulated cells were investigated. Experiments were performed to investigate external film and internal pore di...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-014-1296-0
更新日期:2015-03-01 00:00:00
abstract::Bacteria from Patagonian Merino wool were isolated to assess their wool-keratinolytic activity and potential for felt-resist treatments. Strains from Bacillus, Exiguobacterium, Deinococcus, and Micrococcus produced wool-degrading enzymes. Bacillus sp. G51 showed the highest wool-keratinolytic activity. LC-MS/MS analys...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-017-1748-4
更新日期:2017-06-01 00:00:00
abstract::High amounts of outer membrane (OM) components were released in glucose-limited fed-batch (GLFB) cultures at 37 degrees C at specific growth rates approaching 0.05 h(-1). Endotoxin analyses from a 20 degrees C GLFB culture gave similar results. An alternative fermentation technique, the temperature-limited fed-batch (...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-004-0377-x
更新日期:2005-04-01 00:00:00
abstract::Wheat straw is a low-cost feedstock for the production of biofuel. Pretreatment process is an important stage in producing biofuels since it makes the fibers more accessible to enzymatic hydrolysis which is the final step of producing biofuels. Pretreated wheat straw (PWS) slurries are non-Newtonian fluids with yield ...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-018-1959-3
更新日期:2018-09-01 00:00:00