Abstract：
:The mechanism of thermal inactivation about xanthine oxidase (XOD) from Arthrobacter M3 was investigated. Results of reducing SDS-PAGE indicated that the inactivation of XOD was not related to the peptide degradation. Meanwhile, fluorimetry and circular dichroism spectroscopy suggested that XOD inactivation might be associated with the exposure of hydrophobic residues to surface and partial loss of secondary structure. Specific formation of soluble aggregates of XOD was detected by size exclusion chromatography. In addition, the thermal-dynamic analysis showed that the inactivation kinetics of XOD followed the first-order model. Therefore, trehalose (cosolute) and betaine (osmolyte) were accordingly employed to attenuate the inactivation of this enzyme. The results associated with these two reagents further confirmed that the loss of XOD activity was mainly due to the exposure of hydrophobic residues and formation of aggregation. Owing to the added trehalose and betaine, half-life could be significantly increased, and the inactivation rate constant (k) was detected as decreased.
journal_name
Bioprocess Biosyst Engjournal_title
Bioprocess and biosystems engineeringauthors
Zhang Y,Xin Y,Yang H,Zhang L,Xia X,Tong Y,Chen Y,Wang Wdoi
10.1007/s00449-013-1042-zsubject
Has Abstractpub_date
2014-04-01 00:00:00pages
719-25issue
4eissn
1615-7591issn
1615-7605journal_volume
37pub_type
杂志文章abstract：:ε-Poly-L-lysine (ε-PL), one of the only two homo-poly amino acids known in nature, is used as a preservative. In this study, strategies of feeding precursor L-lysine into 5 L laboratory scale fermenters, including optimization of L-lysine concentration and time, was investigated to optimize the production of ε-PL by S...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-013-0958-7
更新日期：2013-12-01 00:00:00
abstract：:In this study, citric acid production from extract of Jerusalem artichoke tubers by the genetically engineered yeast Yarrowia lipolytica strain 30 was investigated. After the compositions of the extract of Jerusalem artichoke tubers for citric acid production were optimized, the results showed that natural components ...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-013-0951-1
更新日期：2013-11-01 00:00:00
abstract：:Salmonella is a major pathogen that causes acute foodborne outbreaks worldwide. Seafood, particularly shellfish, is a proven source of Salmonella spp. infection because many people prefer to eat it raw or lightly cooked. However, traditional identification methods are too time-consuming and complex to detect contamina...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-018-1895-2
更新日期：2018-05-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-016-1707-5
更新日期：2017-03-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
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更新日期：2013-11-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-019-02245-z
更新日期：2020-03-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-012-0724-2
更新日期：2012-10-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
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更新日期：2007-05-01 00:00:00
abstract：:Menaquinone-7 (MK-7), a subtype of vitamin K, has received a significant attention due to its effect on improving bone and cardiovascular health. Current fermentation strategies, which involve static fermentation without aeration or agitation, are associated with low productivity and scale-up issues and hardly justify...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-017-1807-x
更新日期：2017-10-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
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更新日期：2019-03-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-011-0630-z
更新日期：2012-01-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-018-1929-9
更新日期：2018-07-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-015-1394-7
更新日期：2015-08-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
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doi：10.1007/s00449-015-1465-9
更新日期：2015-12-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
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更新日期：2003-12-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
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更新日期：2009-06-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
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journal_title：Bioprocess and biosystems engineering
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journal_title：Bioprocess and biosystems engineering
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更新日期：2014-08-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s004490100268
更新日期：2002-04-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
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更新日期：2019-05-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-020-02411-8
更新日期：2020-12-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章,评审
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更新日期：2018-01-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-013-0938-y
更新日期：2013-11-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-017-1745-7
更新日期：2017-06-01 00:00:00
abstract：:The gold-rhenium (AuRe) composite nanoparticle was prepared by NaBH(4) reduction procedure, and was modified by the aptamer to obtain an AuRe nanoprobe (AuRessDNA) for thrombin. In pH 7.0 Tris-HCl buffer solution and in the presence of salt, the nanoprobe specifically combined with thrombin to form AuRe-aptamer-thromb...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-010-0434-6
更新日期：2010-11-01 00:00:00
abstract：:Based on the screening of biocatalysts and reaction conditions including solvent, water content, temperature, enzyme loading, and reaction time, lipase from porcine pancreas (PPL) showed the prominent promiscuity for the Knoevenagel condensation between 1,3-dihydroindol-2-one heterocycle and aromatic aldehydes. Under ...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-015-1496-2
更新日期：2016-01-01 00:00:00
