Abstract：
BACKGROUND/AIM:DNA methylation is a common epigenetic change in cancer. However, microRNA (miRNA) regulation by epigenetic alteration, especially CpG island hypermethylation, remains poorly understood in esophageal squamous cell carcinoma (ESCC). MATERIALS AND METHODS:miRNAs which were up-regulated after de-methylation with 5-aza-2'-deoxycytidine (5-AZA) were analyzed using the Human miFinder 384HC miScript miRNA PCR Array. The DNA methylation level was evaluated by bisulfite-pyrosequencing assay. RESULTS:In two of the cell lines, 20 miRNAs, including miR-145-5p, were found to be up-regulated by more than three-fold after 5-AZA treatment. The miRNA-145 promoter was significantly more hypermethylated in the cancer tissues than in matched normal adjacent esophageal epithelial mucosa (p=0.0042; paired t-test). Moreover, the miRNA-145-5p expression levels were significantly lower in cancerous tissues (p=0.0024). CONCLUSION:miRNA-145 expression in ESCC seems to be regulated by hypermethylation of the miRNA-145 promoter region.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Harada K,Baba Y,Ishimoto T,Kosumi K,Tokunaga R,Izumi D,Ohuchi M,Nakamura K,Kiyozumi Y,Kurashige J,Iwagami S,Miyamoto Y,Sakamoto Y,Yoshida N,Oki E,Watanabe M,Baba Hsubject
Has Abstractpub_date
2015-09-01 00:00:00pages
4617-24issue
9eissn
0250-7005issn
1791-7530pii
35/9/4617journal_volume
35pub_type
杂志文章abstract：:High-performance liquid chromatography revealed that sodium 5,6-benzylidene-L-ascorbate (SBA), dissolved in distilled water, was gradually decomposed into ascorbic acid and benzaldehyde. Among these three compounds, ascorbic acid showed the most potent cytotoxic activity. The cytotoxic activity of each compound was si...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1994-05-01 00:00:00
abstract：:The purpose of this study was to determine whether the degree of anchorage-independent growth of rodent or human cells in increasing concentrations of agarose correlated with successful transfection of the cells with an activated c-Ha-ras oncogene and tumorigenicity in nude mice. NIH 3T3 cells, C3H 10T1/2 fibroblasts,...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
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journal_title：Anticancer research
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doi：
更新日期：2009-06-01 00:00:00
abstract：BACKGROUND:The concentration of Des-gamma-carboxy-prothrombin (DCP) or PIVKA-II has been described to be increased in patients with hepatocellular cancer, along with its elevation in vitamin K deficient states by warfarin or dicoumarol treatment. The aim of the study was to investigate its clinical value in HCC in comp...
journal_title：Anticancer research
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doi：
更新日期：1999-07-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2012-05-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1991-03-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1994-09-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1989-07-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2004-09-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2011-06-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2011-07-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2013-07-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2015-05-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2012-04-01 00:00:00
abstract：:Four human melanoma cell lines with different copy numbers of chromosomes 9 and 21q, as studied by the G-band technique, fluorescent in situ hybridisation (FISH) and Polymerase chain reaction (PCR), were tested for their sensitivity to Interferon-alpha (IFN-alpha) and Interferon-beta (IFN-beta) in relation to dosage o...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1996-03-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2015-07-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2016-06-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2008-07-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：10.21873/anticanres.12582
更新日期：2018-05-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2012-05-01 00:00:00
abstract：BACKGROUND:Abnormal hypermethylation of the CDKN2A (p14ARF and p16INK4a) gene can lead to repression of gene expression and contribute to carcinogenesis and tumor progression. MATERIALS AND METHODS:In esophageal squamous cell carcinoma (ESCC), the promoter methylation of the p14ARF and p16INK4a gene was investigated i...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2007-09-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.13532
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-05-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2006-05-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2005-07-01 00:00:00
abstract：BACKGROUND/AIM:Osteosarcoma originates from mesenchymal stem cells with impaired bone differentiation. In the present study we investigated the effect of ascorbic acid (AsA) on osteogenic differentiation and apoptosis of the MG-63 osteosarcoma cell line. MATERIALS AND METHODS:We evaluated the expression of runt-relate...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-04-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1990-03-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.11968
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1992-09-01 00:00:00
