Comparison of Cold Atmospheric Plasma Devices' Efficacy on Osteosarcoma and Fibroblastic In Vitro Cell Models.

Abstract:

BACKGROUND/AIM:Cold atmospheric plasma (CAP) attenuates tumor cell proliferation and induces apoptosis in various cell lines. While exerting marginal effects on non-neoplastic cells this unfolds promising applications in cancer therapy. The aim of the study was to analyse the effects of different CAP sources and application times on osteosarcoma (OS) cells and non-malignant fibroblast cell proliferation. MATERIALS AND METHODS:U2-OS and 3-T-3 fibroblasts were treated with three different approved medical devices. Carrier gas-treated cells served as controls. Cell proliferation was determined by viable cell count at different time points after treatment. RESULTS:Control exposed U2-OS and 3-T-3 cells exhibited characteristic cell growth. CAP application of U2-OS and 3-T-3 cells attenuated proliferation rates up to 98%. Attenuation rates varied between cell lines, plasma sources and application times. CONCLUSION:CAP treatment attenuates cell proliferation of OS cancer cells and fibroblasts in a treatment time-dependent manner, whereby U2-OS cells appeared more sensitive to CAP treatment as 3T3 fibroblasts after 10 sec of treatment.

journal_name

Anticancer Res

journal_title

Anticancer research

authors

Gümbel D,Suchy B,Wien L,Gelbrich N,Napp M,Kramer A,Ekkernkamp A,Daeschlein G,Stope MB

doi

10.21873/anticanres.11968

subject

Has Abstract

pub_date

2017-10-01 00:00:00

pages

5407-5414

issue

10

eissn

0250-7005

issn

1791-7530

pii

37/10/5407

journal_volume

37

pub_type

杂志文章
  • A Scoring System to Predict the Development of Bone Metastasis After Radical Resection of Colorectal Cancer.

    abstract:BACKGROUND/AIM:To develop a scoring system to predict bone metastasis after radical resection within 5 years. PATIENTS AND METHODS:We evaluated the patient records of 1,749 patients, of whom 50 patients developed bone metastasis. Treatment-related factors (age, gender, localization, histology, preoperative carbohydrat...

    journal_title:Anticancer research

    pub_type: 杂志文章

    doi:10.21873/anticanres.11938

    authors: Li A,Käsmann L,Rades D,Fu C

    更新日期:2017-09-01 00:00:00

  • Oncogene transfection of mink lung cells: effect on growth characteristics in vitro and in vivo.

    abstract::Three sublines have been derived from the parental line Mv1Lu by transfection with normal and mutated Ha-ras, and myc oncogenes, and subsequent cloning. All the oncogenes have increased the growth rate of the cell in vitro, increased their plating efficiency in monolayer and suspension, and reduced their serum depende...

    journal_title:Anticancer research

    pub_type: 杂志文章

    doi:

    authors: Khan MZ,Spandidos DA,Kerr DJ,McNicol AM,Lang JC,De Ridder L,Freshney RI

    更新日期:1991-05-01 00:00:00

  • Antimutagenicity of lignin in vitro.

    abstract::The inhibitory activity of lignin against nitrosoguanidine (MNNG)- and acridine orange (AO)- induced mutagenesis was examined using two microbial systems: green unicellular flagellate Euglena gracilis and Salmonella typhimurium TA100 and TA97. To verify the hypothesis that the above mentioned mutagens may generate som...

    journal_title:Anticancer research

    pub_type: 杂志文章

    doi:

    authors: Ebringer L,Krizková L,Polónyi J,Dobias J,Lahitová N

    更新日期:1999-01-01 00:00:00

  • Specific detection of circulating tumor cells by reverse transcriptase-polymerase chain reaction of a beta-casein-like protein, preferentially expressed in malignant neoplasms.

    abstract::Beta-casein-like protein (BCLP) is a putative protein on cervical cancer and exhibits immunological characteristics similar to those of bovine beta-casein. We evaluated if BCLP mRNA detection in the blood is useful in gynecologic malignancies. We examined 30 patients with uterine cancer, nine cultured cancer cell line...

    journal_title:Anticancer research

    pub_type: 杂志文章

    doi:

    authors: Baba T,Koizumi M,Suzuki T,Yamanaka I,Yamashita S,Kudo R

    更新日期:2001-07-01 00:00:00

  • Concomitant radiochemotherapy with temozolomide in non-selected patients with newly diagnosed high-grade gliomas.

    abstract:BACKGROUND:Malignant gliomas still present a medical challenge, despite decades of continuous extensive research with optimization of surgical techniques, radiotherapy and systemic treatments. PATIENTS AND METHODS:From 1999 to 2004, 104 patients with WHO grade III and IVgliomas underwent surgery and received concomita...

    journal_title:Anticancer research

    pub_type: 杂志文章

    doi:

    authors: Eberlein KH,Nagel B,Franz K,Imhoff D,Seifert V,Boettcher HD,Mose S

    更新日期:2006-11-01 00:00:00

  • Enhanced effect of combined hyperthermia and chemotherapy (bleomycin, BCNU) in a neurogenic rat tumour (BT4A) in vivo.

    abstract::The neurogenic rat tumour BT4A was serially transplanted to the dorsum of the right hind leg of BD IX rats. The tumours were treated with water bath heating at 44.0 degrees C for one hour alone or combined with bleomycin or BCNU, both given intraperitoneally at a dose of 20 mg/kg. Bleomycin alone had no effect on tumo...

    journal_title:Anticancer research

    pub_type: 杂志文章

    doi:

    authors: Dahl O,Mella O

    更新日期:1982-11-01 00:00:00

  • Potent Antitumor T-Cell Memory Is Generated by Curative Viral Oncolytic Immunotherapy But Not Curative Chemotherapy.

    abstract:BACKGROUND:Late developing breast cancer metastases are common and lethal despite treatment with adjuvant chemotherapy at the time of primary tumor excision. Stimulation of an antitumor immune response is an alternative strategy for preventing this devastating development. MATERIALS AND METHODS:A mouse model of the hu...

    journal_title:Anticancer research

    pub_type: 杂志文章

    doi:10.21873/anticanres.13029

    authors: Gao Y,Bergman I

    更新日期:2018-12-01 00:00:00

  • Novel system for generating cytotoxic effector lymphocytes using carcinoembryonic antigen (CEA) peptide and cultured dendritic cells.

    abstract::We have established a practical system for generating antitumor effector lymphocytes using the tumor antigen peptide CEA and cultured dendritic cells (DCs), and have also characterized effector cells. DCs were induced from the adherent cell population of autologous peripheral blood mononuclear cells (PBMCs) obtained f...

    journal_title:Anticancer research

    pub_type: 杂志文章

    doi:

    authors: Ohta K,Yamaguchi Y,Shimizu K,Miyahara E,Toge T

    更新日期:2002-09-01 00:00:00

  • Analysis of EBV latency by EBER in situ hybridization in nasopharyngeal carcinoma Spanish patients.

    abstract:BACKGROUND:The aim of this study was to analyse the Epstein-Barr virus (EBV) latency by detecting the EBV-associated latent small nuclear RNAs (EBER), in a group of biopsies from Spanish patients with diagnosed nasopharyngeal carcinoma (NPC). MATERIALS AND METHODS:NPC paraffin samples with the presence of EBV demonstr...

    journal_title:Anticancer research

    pub_type: 杂志文章

    doi:

    authors: Burgos JS,Vera-Sempere FJ

    更新日期:2001-11-01 00:00:00

  • Involvement of Corticotropin-releasing Hormone-related Peptides in Cellular Stress Caused by Anticancer Drugs in Colorectal Cancer.

    abstract:BACKGROUND/AIM:The expression of corticotropin-releasing hormone (CRH)-related peptides involved in stress response in colorectal cancer has been reported. We examined the involvement of CRH-related peptides in cellular stress caused by anticancer drugs in colorectal cancer. MATERIALS AND METHODS:Changes in the expres...

    journal_title:Anticancer research

    pub_type: 杂志文章

    doi:10.21873/anticanres.14455

    authors: Kuwata GO,Ikeda K,Manome Y

    更新日期:2020-08-01 00:00:00

  • Significance of Elevated HMGB1 Expression in Pituitary Apoplexy.

    abstract:BACKGROUND/AIM:High-mobility group box 1 (HMGB1) is a nuclear DNA-binding protein that exerts a range of proinflammatory actions when it is secreted extracellularly. We hypothesized that HMGB1 released from damaged cells in pituitary apoplexy would exacerbate the neurological symptoms due to acute inflammation. PATIEN...

    journal_title:Anticancer research

    pub_type: 杂志文章

    doi:10.21873/anticanres.13624

    authors: Okuda T,Fujita M,Kato A

    更新日期:2019-08-01 00:00:00

  • MEK and p38MAPK inhibitors potentiate TNF-alpha induced apoptosis in U937 cells.

    abstract:BACKGROUND:TNF-alpha is one of the key inflammatory cytokines and it modulates various events through several pathways. U937 myelomonocytic leukemia cells are sensitive to TNF-alpha and about 20% of these cells undergo apoptosis within 6 hours after treatment. Co-treatment of these cells with actinomycin D or cyclohexi...

    journal_title:Anticancer research

    pub_type: 杂志文章

    doi:

    authors: Nakada S,Kawano T,Saito-akita S,Iwase S,Horiguchi-Yamada J,Ohno T,Yamada H

    更新日期:2001-01-01 00:00:00

  • Thymidine kinase levels correlate with prognosis in aggressive lymphoma and can discriminate patients with a clinical suspicion of indolent to aggressive transformation.

    abstract::Serum levels of thymidine kinase 1 (TK1), an enzyme involved in the G1-S phase of the cell cycle, have been previously shown to correlate with the prognosis of lymphoid malignancies. We hypothesized that TK1 levels will be higher in aggressive, compared to indolent lymphoproliferative, malignancies and this may serve ...

    journal_title:Anticancer research

    pub_type: 杂志文章

    doi:

    authors: Gatt ME,Goldschmidt N,Kalichman I,Friedman M,Arronson AC,Barak V

    更新日期:2015-05-01 00:00:00

  • Topotecan-induced alterations in the amount and stability of human DNA topoisomerase I in solid tumor cell lines.

    abstract:BACKGROUND:Human DNA topoisomerase I (topo 1) is an essential nuclear enzyme involved in vital cellular processes and the sole target of antitumor drugs of the camptothecin (CPT) family. The CPT derivative topotecan (Tpt, Hycamtin) is currently used in clinic, its effectiveness varying considerably for different types ...

    journal_title:Anticancer research

    pub_type: 杂志文章

    doi:

    authors: Devy J,Wargnier R,Pluot M,Nabiev I,Sukhanova A

    更新日期:2004-05-01 00:00:00

  • Clinical significance of vimentin-positive gastric cancer cells.

    abstract:BACKGROUND/AIM:Vimentin expression in epithelial cells is reported to be associated with the malignant phenotype of cancer cells in vitro. However, the clinical significance of vimentin expression in carcinomas is not well understood. The aim of this study was to clarify the significance of vimentin-positive gastric ca...

    journal_title:Anticancer research

    pub_type: 杂志文章

    doi:

    authors: Fuyuhiro Y,Yashiro M,Noda S,Kashiwagi S,Matsuoka J,Doi Y,Kato Y,Kubo N,Ohira M,Hirakawa K

    更新日期:2010-12-01 00:00:00

  • Diffusion-weighted imaging using 3.0 T MRI as a possible biomarker of renal tumors.

    abstract:BACKGROUND/AIM:Diffusion-weighted imaging (DWI) allows for differentiation of benign from malignant tumors, histological tumor types and their grade. The aim of the study was to evaluate the capabilities of DWI using 3 Tesla Magnetic resonance imaging (3T MRI) in the preoperative assessment of renal tumors. PATIENTS A...

    journal_title:Anticancer research

    pub_type: 杂志文章

    doi:

    authors: Mirka H,Korcakova E,Kastner J,Hora M,Hes O,Hosek P,Ferda J

    更新日期:2015-04-01 00:00:00

  • A paclitaxel-containing chemotherapy does not cause central nervous adverse effects: a prospective study in patients with ovarian cancer.

    abstract:BACKGROUND:The objective of this study was to evaluate the possible effects of a paclitaxel containing chemotherapy on different neuropsychological parameters in women with ovarian cancer. MATERIALS AND METHODS:Twenty-eight women with histologically documented epithelial ovarian carcinoma and treated with a combinatio...

    journal_title:Anticancer research

    pub_type: 临床试验,杂志文章

    doi:

    authors: Mayerhofer K,Bodner-Adler B,Bodner K,Saletu B,Schindl M,Kaider A,Hefler L,Leodolter S,Kainz C

    更新日期:2000-09-01 00:00:00

  • Interphase FISH for follow-up of Philadelphia chromosome-positive chronic myeloid leukemia treatment.

    abstract:BACKGROUND:Several attempts have been made to determine whether interphase fluorescence in situ hybridization (I-FISH) on bone marrow or peripheral blood specimens is a good alternative to conventional cytogenetics (CC) in calculating the residual proportion of Philadelphia (Ph) chromosome-positive cells during treatme...

    journal_title:Anticancer research

    pub_type: 杂志文章

    doi:

    authors: Douet-Guilbert N,Morel F,Le Charpentier T,Le Bris MJ,Herry A,Morice P,Bourquard P,Abgrall JF,Berthou C,De Braekeleer M

    更新日期:2004-07-01 00:00:00

  • Carcinoma of the ampulla of Vater in Crete. A clinical and ERCP registry over eight years.

    abstract:BACKGROUND:Carcinoma of the ampulla of Vater is an infrequent tumor that can be diagnosed, early. PATIENTS AND METHODS:Twenty-four patients with histologically proven carcinoma of the ampulla of Vater were retrospectively studied and their data were analyzed. RESULTS:Most common presenting symptoms were jaundice (67%...

    journal_title:Anticancer research

    pub_type: 杂志文章

    doi:

    authors: Mouzas IA,Skordilis P,Frangiadakis N,Leondidis C,Alexandrakis G,Potamianos S,Kouroumalis E,Manousos ON

    更新日期:1999-09-01 00:00:00

  • Effect of ALP analogs on inositol trisphosphate formation in H184 mammary epithelial cells before and after transfection with v-erb B oncogene.

    abstract::The influence of cytostatically active alkyllysophospholipid analogs with different chemical structure on IP3 (inositol-1,4,5-trisphosphate) formation and intracellular Ca++ concentration was studied in human mammary epithelial cells before and after transfection with v-erb B oncogene DNA. Transformed cells showed an ...

    journal_title:Anticancer research

    pub_type: 杂志文章

    doi:

    authors: Junghahn I,Bergmann J,Langen P,Thun I,Vollgraf C,Brachwitz H

    更新日期:1995-03-01 00:00:00

  • Aneuploidy of chromosome 8 and C-MYC amplification in individuals from northern Brazil with gastric adenocarcinoma.

    abstract:BACKGROUND:Gastric cancer is the third most frequent type of neoplasia. In northern Brazil, the State of Pará has a high incidence of this type of neoplasia. Limited data are available so far on the genetic events involved in this disease. MATERIALS AND METHODS:Dual-color fluorescence in situ hybridization (FISH) for ...

    journal_title:Anticancer research

    pub_type: 杂志文章

    doi:

    authors: Calcagno DQ,Leal MF,Taken SS,Assumpção PP,Demachki S,Smith Mde A,Burbano RR

    更新日期:2005-11-01 00:00:00

  • Combination chemotherapy with paclitaxel and gemcitabine followed by concurrent chemoradiotherapy in non-operable localized non-small cell lung cancer. A hellenic cooperative oncology group (HeCOG) phase II study.

    abstract:UNLABELLED:Concurrent chemoradiotherapy has become a standard therapy for locoregionally advanced inoperable nonsmall cell lung cancer (NSCLC). The purpose of this phase II trial was to evaluate the efficacy and toxicity of concurrent chemoradiotherapy following induction with non-platinum chemotherapy in patients with...

    journal_title:Anticancer research

    pub_type: 杂志文章

    doi:

    authors: Kosmidis P,Fountzilas G,Baka S,Samantas E,Dimopoulos AM,Gogas H,Skarlos D,Papacostas P,Boukovinas J,Bakogiannis Ch,Pantelakos P,Athanasiou H,Misailidou D,Tsekeris P,Pavlidis N

    更新日期:2007-11-01 00:00:00

  • Antiproliferative activity of vitamin D compounds in combination with cytostatics.

    abstract::Calcitriol is a potent antiproliferative agent against various tumour cells in vitro. Here, the results of a study on vitamin D compounds (calcitriol's analogues PRI-1906 and PRI-2191) as potential agents in combined antitumour therapy in vitro are presented. Applying antiproliferative SRB and MTT assays, the growth i...

    journal_title:Anticancer research

    pub_type: 杂志文章

    doi:

    authors: Pelczynska M,Switalska M,Maciejewska M,Jaroszewicz I,Kutner A,Opolski A

    更新日期:2006-07-01 00:00:00

  • Immunocytochemical expression of Ki67 and laminin in Hurthle cell adenomas and carcinomas.

    abstract:BACKGROUND:Hurthle cell neoplasms may occur as a benign adenoma or carcinoma; the latter displays comparatively aggressive clinical behaviour. Fine-needle aspiration cytology (FNAC) represents a reliable tool for screening Hurthle cell lesions before surgery. Nevertheless, the cytological interpretation of these lesion...

    journal_title:Anticancer research

    pub_type: 杂志文章

    doi:

    authors: Pisani T,Pantellini F,Centanni M,Vecchione A,Giovagnoli MR

    更新日期:2003-07-01 00:00:00

  • Antimutagenic action of methionine in Chinese hamster fibroblasts: an opposite of methionine mutagenicity.

    abstract::Serially propagated fibroblast clones were treated with 25-mM methionine and a thioguanine resistance assay was performed at each passage to measure mutagenesis at the HGPRT locus. The spontaneous frequency of thioguanine resistant mutants before treatment was less than 5 X 10(-6) in four clones and 22 X 10(-6) in one...

    journal_title:Anticancer research

    pub_type: 杂志文章

    doi:

    authors: Hill M,Hillova J,Mariage-Samson R,Brada Z

    更新日期:1988-07-01 00:00:00

  • L-histidinol increases the vulnerability of cultured human leukemia and lymphoma cells to anticancer drugs.

    abstract::L-Histidinol, a structural analogue of the essential amino acid L-histidine, enhances the toxicity of a variety of anticancer drugs for many tumor cells of animal origin. In this study, the effects of L-histidinol on the proliferation and anticancer drug susceptibility of two human tumor cell lines of lymphoid origin,...

    journal_title:Anticancer research

    pub_type: 杂志文章

    doi:

    authors: Warrington RC,Cheng I,Zhang L,Fang WD

    更新日期:1993-11-01 00:00:00

  • Cancer immunotherapy by intratumoral injection of α-gal glycolipids.

    abstract:UNLABELLED:AIM/ BACKGROUND: To determine the feasibility and safety of intratumoral α-gal glycolipids injection for conversion of human tumors into autologous Tumor Associated Antigens (TAA) vaccine. α-Gal glycolipids bind anti-Gal--the most abundant antibody in humans. Pre-clinical studies indicated that injected α-ga...

    journal_title:Anticancer research

    pub_type: 杂志文章

    doi:

    authors: Whalen GF,Sullivan M,Piperdi B,Wasseff W,Galili U

    更新日期:2012-09-01 00:00:00

  • Beta-adrenergic influences on doxorubicin-sensitive or -resistant P388 leukemia cells.

    abstract::Taking into account the possible regulatory influences of the beta-adrenergic system on lymphocyte proliferation as well as the proposed role of cyclic 3'-5'-adenosine monophosphate (cAMP) in the modulation of multidrug resistance (MDR) in tumour cells, we have tried to assess the status of the interactions between th...

    journal_title:Anticancer research

    pub_type: 杂志文章

    doi:

    authors: D'Amico C,Crescimanno M,Armata MG,Leonardi V,Palazzoadriano M,D'Alessandro N

    更新日期:1992-11-01 00:00:00

  • Anticancer Effects of Colchicine on Hypopharyngeal Cancer.

    abstract::Colchicine is an alkaloid widely used for the treatment of inflammatory diseases, such as gout. It suppresses cell division by inhibiting mitosis. We investigated the anticancer effects of colchicine on human hypopharyngeal cancer cells and the mechanisms underlying its anticancer effects. XTT cell proliferation assay...

    journal_title:Anticancer research

    pub_type: 杂志文章

    doi:10.21873/anticanres.12078

    authors: Cho JH,Joo YH,Shin EY,Park EJ,Kim MS

    更新日期:2017-11-01 00:00:00

  • Interferon response depends on viral transcription in human papillomavirus-containing lesions.

    abstract::Human papillomaviruses (HPVs) express various proteins which have been proven to interact with some cellular functions playing a role in cell cycle progression and differentiation. Therefore, these viral gene products might be candidates for interfering with IFN-mediated antiproliferative actions. Condyloma biopsies f...

    journal_title:Anticancer research

    pub_type: 杂志文章

    doi:

    authors: Arany I,Goel A,Tyring SK

    更新日期:1995-11-01 00:00:00