Abstract:
BACKGROUND:Abnormal hypermethylation of the CDKN2A (p14ARF and p16INK4a) gene can lead to repression of gene expression and contribute to carcinogenesis and tumor progression. MATERIALS AND METHODS:In esophageal squamous cell carcinoma (ESCC), the promoter methylation of the p14ARF and p16INK4a gene was investigated in 38 cases by methylation-specific PCR and the expression of each gene in 18 cases was quantified by real-time quantitative reverse transcription-PCR. RESULTS:Aberrant methylation of p14ARF and of p16INK4a was detected in 23 (61%) and 29 (76%) cases, respectively. No relationship was found between clinicopathological variables and p14ARF or p161NK4a promoter methylation. A statistically significant association between p14ARF methylation status and mRNA expression was found (p=0.0441). Regarding p14ARF, a low expression group showed a significantly higher proportion of cases with deep invasion of tumor, lymph node metastasis, and a high TNM stage of disease (p=0.0474, 0.0474, and 0.0441, respectively), and a significantly poor prognosis (p=0.0402). Regarding p161NK4a, no relationship was found among the methylation status, mRNA expression and clinicopathological variables, including survival. CONCLUSION:Our results suggest that methylation is the predominant mechanism of inactivation of the p14ARF gene in ESCC. The decrease in p14ARF gene expression associated with invasive and metastatic phenotypes may be significant as an indicator of the malignant potential of human ESCC.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Ito S,Ohga T,Saeki H,Watanabe M,Kakeji Y,Morita M,Yamada T,Maehara Ysubject
Has Abstractpub_date
2007-09-01 00:00:00pages
3345-53issue
5Aeissn
0250-7005issn
1791-7530journal_volume
27pub_type
杂志文章abstract::Combination therapies are used in the clinic to achieve cure, better efficacy and to circumvent resistant disease in patients. Initial assessment of the effect of such combinations, usually of two agents, is frequently performed using in vitro assays. In this review, we give a short summary of the types of analyses th...
journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:10.21873/anticanres.13472
更新日期:2019-07-01 00:00:00
abstract:BACKGROUND:One of the challenges to improving access to care is identifying disparities in health care. To determine the influence of insurance status on outcome for patients with non-small cell lung cancer (NSCLC), we analyzed data from the National Cancer Data Base (NCDB) from 1998-2011. MATERIALS AND METHODS:Data f...
journal_title:Anticancer research
pub_type: 历史文章,杂志文章
doi:
更新日期:2016-01-01 00:00:00
abstract:AIM:Carbonic anhydrase 9 (CA9) has been found to be one of most powerful biomarkers for clear-cell renal cell carcinoma (RCC). The serum CA9 is detectable. The aim of this study was to evaluate the potential prognostic role of serum CA9 in patients with metastatic clear-cell RCC patients under targeted therapy. PATIEN...
journal_title:Anticancer research
pub_type: 杂志文章,随机对照试验
doi:
更新日期:2012-12-01 00:00:00
abstract::To evaluate the effects of dietary fats on breast cancer growth and metastasis, KPL-1 human breast carcinoma cells which have a propensity for axillary lymph node metastasis when inoculated into the thoracic mammary fat pad of female nude mice were examined. The mice were fed one of three semipurified diets containing...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1998-05-01 00:00:00
abstract::A new cell line, designated CC2/CUHK2, was established from a squamous carcinoma of the uterine cervix from a Chinese patient. The cell line grew well without interruption for over 12 months and over 90 passages. The doubling time of CC2/CUHK2 was 72 hours at passage 57. When seeded at a density of 1.5 x 10(3) cells p...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1993-07-01 00:00:00
abstract::The interaction between sodium 5,6-benzylidene-L-ascorbate (SBA) and dopamine was investigated by three different parameters: radical intensity, prooxidant action and cytotoxic activity. Under alkaline conditions, SBA and dopamine produced doublet and quartet ESR signals, respectively. The addition of increasing conce...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1998-09-01 00:00:00
abstract::The incidence of female breast cancer was studied as a function of age in 71 different populations throughout the world. Incidence data was normalized for differences in frequency of occurrence. Median normalized incidence increased exponentially from age 20 to 45. After age 45 the rate of incidence decreased, and we ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1982-01-01 00:00:00
abstract:BACKGROUND:Immmunosuppression and infectious disease in cancer patients receiving chemotherapy is a serious problem. Immunopotentiating drugs may show a therapeutic efficacy. MATERIALS AND METHODS:The protective effect of Hochu-ekki-to (TJ-41), a Japanese traditional herbal medicine, on mitomycin C (MMC)-induced immun...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2000-11-01 00:00:00
abstract::The human MAGE gene products are recognized by major histocompatibility complex-restricted cytotoxic T lymphocytes. We analyzed by RT-PCR the expression of MAGE-1, MAGE-2, MAGE-3 and HLA-A2 genes in 10 human gastric cancer cell lines and 46 human stomachs removed due to advanced gastric carcinomas. All the cell lines ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1998-09-01 00:00:00
abstract::The incorporation and metabolism of delta-6-desaturase substrate and product, [1-14C]-linoleic (18:2n-6) and [1-14C]-gamma-linolenic acid (18:3n-6), was examined in NIH-3T3 cells and the DT subclone which differs only in the presence of the v-Ki-ras oncogene. Similar amounts of post delta-6 and delta-5 desaturase meta...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1993-07-01 00:00:00
abstract:BACKGROUND/AIM:Elderly cancer patients are more prevalent and require special attention. This study focused on the outcome of elderly (≥65 years) rectal cancer patients treated with tri-modality therapy. PATIENTS AND METHODS:A total of 105 patients receiving neoadjuvant radio-chemotherapy and resection for locally adv...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.13830
更新日期:2019-11-01 00:00:00
abstract:AIM:Human papillomavirus (HPV) is a risk factor for oropharyngeal squamous cell carcinoma (OPSCC), with an increasing incidence. The present study aimed to determine the changing incidence of HPV in patients with OPSCC in the period 1980-2009 and its influence on survival. PATIENTS AND METHODS:We randomly sampled 158 ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2015-07-01 00:00:00
abstract:BACKGROUND:Amrubicin is an active agent for the treatment of small-cell lung cancer (SCLC). However, there have been no reports of long-term amrubicin use. PATIENTS AND METHODS:Twelve patients with SCLC who were treated with eight or more cycles of amrubicin chemotherapy were retrospectively reviewed. RESULTS:The med...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2012-04-01 00:00:00
abstract:BACKGROUND/AIM:Magnetic resonance (MR) and ultrasound (US) fusion imaging (MR-US fusion) is already used to guide prostate biopsies and has been proven accurate for diagnosing cervical cancer. In this study, we aimed to evaluate the feasibility and performance of MR-US fusion for characterizing adnexal masses. PATIENT...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.14410
更新日期:2020-07-01 00:00:00
abstract:BACKGROUND:We evaluated the effect of in vivo electrogene therapy (EGT), a newly-developed gene transfer method using electroporation on the induction of anti-cancer immunity. MATERIALS AND METHODS:The in vivo EGT was carried out by direct injection of plasmid DNAs encoding mouse interleukin-2 (IL-2) and granulocyte-m...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::A 70-year-old man with a growing liver tumor had undergone subtotal gastrectomy with pancreaticoduodenectomy for gastric cancer (T4b P0 H0 N1, Stage IIIB) 30 months before admission to our hospital. Enhanced computed tomography revealed two hypervascular nodules in segments 4 and 8. After histological diagnosis of sma...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.12389
更新日期:2018-03-01 00:00:00
abstract:BACKGROUND/AIM:Docking protein 2 (Dok2) is an adapter protein which is involved in hematopoiesis. However, it still remains unclear how Dok2 functions in regulation of transcription of hematopoietic genes. To address this issue, we knocked-down Dok2 mRNA in mouse erythroleukemia cells which highly express Dok2 intrinsi...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2014-08-01 00:00:00
abstract:BACKGROUND:The transforming growth factor beta (TGF-beta) superfamily regulates cell growth in various cells. Previous studies have shown that altered expression of TGF-beta receptors, Smad2 and Smad4, as mediators of TGF-beta superfamily signaling, contributes to tumor progression in esophageal squamous cell carcinoma...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2004-11-01 00:00:00
abstract::Integrin alpha v-beta 3 is involved in tumor angiogenesis while the clinical significance of beta 3 integrin expression in colorectal cancer and lung metastases. Analysis was performed on 51 colorectal cancer patients (22 with subsequent lung metastasis and 29 without lung metastasis). Fifty-one primary tumors and 22 ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:Ethanol injection is widely used in liposome preparation. However, the parameters determining particle size distribution of the liposomal preparation has not been fully defined. MATERIALS AND METHODS:A syringe pump-driven microfluidic injection device was used to produce liposomes under different conditions...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2008-03-01 00:00:00
abstract::The results of palliative chemotherapy in 162 patients suffering from exocrine pancreatic cancer are presented. They are mainly discussed with respect to the possibility of improving survival of exocrine pancreatic cancer patients by an efficacy-orientated sequential polychemotherapy (EOSP). In about 40% of the patien...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2003-03-01 00:00:00
abstract::Rats were inoculated subcutaneously into both flanks with a transplantable adenocarcinoma of the colon. They were treated intravenously with either 5-FUrd (5-fluorouridine) or 5-FdUrd (5-fluoro-2'-deoxyuridine) with or without addition of dipyridamole 20 and 30 min later, respectively, for 3 consecutive days. Dipyrida...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
abstract::The centrifugal spreading of multicellular aggregates from a DMH induced colon adenocarcinoma cell line from rats was observed in vitro. Various concentrations of pentagastrin were added to the culture medium. Controls included medium alone or culture medium containing different concentrations of either NH4OH or NaOH ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1986-03-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2003-07-01 00:00:00
abstract::12-O-retinoylphorbol-13-acetate (RPA), an incomplete tumor promoter of the phorbol ester type and protein kinase C (PKC) activator, consists of two characteristic structural elements: the phorbol body and the retinoyl ester chain. Therefore, possible binding of the incomplete tumor promoter RPA to the human transport ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2005-09-01 00:00:00
abstract:BACKGROUND/AIM:To investigate the impact of inguinal lymph node dissection (ILND) following neoadjuvant chemoradiotherapy (NACRT) for rectal cancer patients with ILN metastasis. PATIENTS AND METHODS:Forty-three patients with rectal cancer underwent NACRT followed by curative surgery between January 2005 and December 2...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.13779
更新日期:2019-10-01 00:00:00
abstract::The aims of this study were to evaluate the pharmacokinetics, tolerability and hematopoietic toxicity of mitoxantrone in elderly women. Thirteen patients with advanced breast cancer, median age of 73 years, received escalating doses of mitoxantrone 8, 10, 12 and 14 mg/m2 on day 1, q 21. There was a linear relationship...
journal_title:Anticancer research
pub_type: 临床试验,杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract::Interferon-alpha (IFN-alpha) has been found to exert multiple enhancing effects in the immune response in vitro, IFN-alpha has been also used in clinical trials with variable response rates. The aim of the present study was to assess the effectiveness of IFN-alpha in the treatment of 25 patients with malignant pleural...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1996-11-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:
更新日期:2010-01-01 00:00:00
abstract::Gross cystic disease (GCD) represents an advanced form of fibrocystic disease of the breast. Bearers of macrocysts have been reported to be at risk of developing breast cancer. Natural killer (NK) cells are a lymphocyte subset deeply involved in immunosurveillance against neoplasia. We investigated whether breast cyst...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1994-03-01 00:00:00