Abstract:
BACKGROUND/AIM:Osteosarcoma originates from mesenchymal stem cells with impaired bone differentiation. In the present study we investigated the effect of ascorbic acid (AsA) on osteogenic differentiation and apoptosis of the MG-63 osteosarcoma cell line. MATERIALS AND METHODS:We evaluated the expression of runt-related transcription factor-2 (RUNX2) and secreted phosphoprotein 1 (SPP1) genes by real-time Polymerase Chain Reaction (PCR) and of endogenous bone morphogenetic protein-2 (BMP2) and osteocalcin proteins by immunohistochemistry. We analyzed osteoblast maturation by phosphatase alkaline synthesis and calcium deposition, and apoptosis by (TUNEL) test and Annexin staining. RESULTS:Our results showed that RUNX2 and SPP1 gene expression was increased in cells treated with low concentrations of AsA with respect to untreated cells. At higher concentrations, AsA induced apoptosis of osteosarcoma cells, possibly with the involvement of p21. CONCLUSION:Our findings support the ability of AsA to induce both differentiation, by affecting the target involved in early and late phases of osteogenic maturation, and apoptosis in poorly-differentiated osteosarcoma cells.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Valenti MT,Zanatta M,Donatelli L,Viviano G,Cavallini C,Scupoli MT,Dalle Carbonare Lsubject
Has Abstractpub_date
2014-04-01 00:00:00pages
1617-27issue
4eissn
0250-7005issn
1791-7530pii
34/4/1617journal_volume
34pub_type
杂志文章abstract::A brief review summarizing the relationships that exist between the DEK protein and gene regulation, oncogenesis and AIDS. ...
journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:
更新日期:2003-05-01 00:00:00
abstract::Growth inhibitory activity of YM881 (SMANCS) against 3 human cultured cell lines and one rat cultured cell line derived from glioma cells was assessed quantitatively. YM881 showed potent cytotoxicity against all glioma cell lines tested; the IC50 of this drug was 1.9-8.4 micrograms/ml. YM881 may be classified as a con...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1991-11-01 00:00:00
abstract::To investigate the specific transduction of a suicide gene into human small cell lung carcinoma (SCLC) cells, we explored the promoter region of the neuron specific enolase (NSE) gene as a tumor-specific promoter. In Northern blot analysis, NSE mRNA was expressed more abundantly in the SBC3 human SCLC cell line than i...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND/AIM:Androgen-androgen receptor (AR) signal is known as a powerful driver of prostate cancer progression. We previously reported the limitation of prostate-specific antigen (PSA) at diagnosis as a prognostic biomarker of prostate cancer. Although serum total testosterone (TT) level has been reported as a prog...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.11988
更新日期:2017-10-01 00:00:00
abstract:BACKGROUND:Increasing evidence suggests that COX-2 promotes carcinogenesis but it is controversial as to whether COX-2 expression is a prognostic factor for rectal carcinoma. MATERIALS AND METHODS:Data of 62 consecutive patients with rectal carcinoma in UICC-stages I-III, which were curatively resected between 1995 an...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2002-03-01 00:00:00
abstract:BACKGROUND:Inhibition of the vascular endothelial growth factor receptor (VEGFR) has been shown to improve the radiation response of several experimental tumors. The present experimental study compares the effect of neoadjuvant, concomitant and adjuvant treatment with PTK787/ZK222584, a specific inhibitor of the VEGFR,...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2003-09-01 00:00:00
abstract:AIM:We hypothesized that a nomogram can accurately predict overall survival (OS) for patients with intrahepatic cholangiocarcinoma (ICC) after hepatectomy. MATERIALS AND METHODS:A nomogram to predict OS based was developed using data from 185 ICC patients who had undergone hepatectomy. The nomogram was evaluated by co...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2016-08-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2012-12-01 00:00:00
abstract:BACKGROUND:Immunotherapy has become an attractive new strategy in the treatment of cancer. The laboratory and clinical study of cancer immunotherapy is rapidly advancing. However, in the clinical setting, the results of cancer immunotherapy are mixed. We therefore contend that cancer immunotherapy should be customized ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2013-07-01 00:00:00
abstract::Prospective studies of dose adaptation of continuous 5FU infusion combined with cisplatin have shown that pharmacologically guided dosing was feasible in the treatment of head and neck carcinomas. Adaptative dosing results in reduced haematological toxicity, but few data are available for clinical response rate. Preli...
journal_title:Anticancer research
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1994-11-01 00:00:00
abstract:AIM:To investigate the expression and significance of glypican-3 (GPC3) in human hepatocellular carcinoma (HCC). MATERIALS AND METHODS:DNA chips were used to measure the expression of mRNAs for members of the glypican and syndecan families of heparan sulfate proteoglycans (HSPGs) in normal liver tissue, non-tumor tiss...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2010-12-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章,多中心研究
doi:
更新日期:2015-01-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2002-03-01 00:00:00
abstract:BACKGROUND/AIM:L-type amino acid transporter 1 (LAT1) is highly expressed in various human cancers. However, the clinicopathological significance of LAT1 and 4F2 cell surface antigen (4F2hc) in patients with colorectal cancer (CRC) is unknown. The aim of this study was to clarify the prognostic significance of LAT1 exp...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.13375
更新日期:2019-05-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:
更新日期:1993-11-01 00:00:00
abstract:BACKGROUND/AIM:Chest radiotherapy (RT) doubles late cardiac mortality. This study aimed to evaluate the evolution of cardiac changes in speckle tracking echocardiography during a three-year follow-up. MATERIALS AND METHODS:This prospective study included 81 chemotherapy-naïve early-stage breast cancer patients who wer...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.13584
更新日期:2019-08-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章,多中心研究
doi:
更新日期:2010-02-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1992-05-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:
更新日期:1994-11-01 00:00:00
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journal_title:Anticancer research
pub_type: 临床试验,杂志文章
doi:
更新日期:2002-03-01 00:00:00
abstract::In order to determine the efficacy of liposomal encapsulated daunorubicin (DaunoXome; DNX) in chronic Iymphocytic leukaemia, the sensitivities of cells from 10 patients with this disease were assessed and compared with that of free drug using the MTT assay. There was a marked variation in effect between patients for b...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1999-07-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2006-05-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2002-09-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2003-11-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:10.21873/anticanres.13461
更新日期:2019-06-01 00:00:00
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journal_title:Anticancer research
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:
更新日期:2002-07-01 00:00:00
abstract::Linomide has antitumor effects when administered in vivo but not in vitro. Recent data indicate that at least part of this effect can be attributed to anti-angiogenic properties. The aim of the present investigation was to study the anti-angiogenic effects of Linomide on early tumor-induced angiogenesis in vivo, using...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1995-05-01 00:00:00
abstract:OBJECTIVE:Laboratory rats can develop benign or malignant tumors (TM) spontaneously or following various carcinogenic processes, e.g. irradiation. The effects of irradiation vary according to the irradiation field (RF), the dosage and the strain of rat. Radiation-induced malignant TM in rats are predominantly sarcomas....
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2000-11-01 00:00:00
abstract::We generated a recombinant immunotoxin, named scFv(MGR6)-Cla, composed of the Fv region of an anti ErbB2 monoclonal antibody (MGR6) fused to clavin, a type 1 ribosome-inactivating protein (RIP) from Aspergillus clavatus. ErbB2 is a tyrosine kinase receptor which is overexpressed in most adenocarcinomas; clavin is a 17...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1998-09-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:
更新日期:1987-09-01 00:00:00