Abstract：
BACKGROUND:The lipid components and natural moisturizing factors (NMFs) of the stratum corneum (SC) are integral pieces of the self-regulating barrier strategy which comprises one of the most important functions of human skin and seems to be related to biomechanical responses of the SC. OBJECTIVES:This work presents the contributions of the lipid bilayers and NMFs to the barrier properties and mechanical responses of human SC. METHODS:We performed 2 biomechanical experiments, substrate curvature testing and double cantilever beam cohesion measurements, on isolated human SC exposed to either water, a 1:1 mixture of acetone/ether, or a 1:1 mixture of chloroform/methanol for various durations. RESULTS:We show that treating ex vivo SC with organic solvents results in lipid extraction which increases with duration of exposure. This extraction is tied to a remarkably linear increase in the levels and rates of biaxial stress development during drying/hydration cycles. This effect appears to be tied to the total amounts of lipids extracted. Furthermore, striking changes are seen in the intercellular cohesion properties of the tissue after solvent exposure. Interestingly, changes in drying stress profiles are not observed after treatment with water, which has been previously shown to remove NMFs from the tissue, and which therefore might be expected to induce changes in the drying behavior of the skin. However, changes in intercellular cohesion and the SC cohesion gradient are seen, suggesting impacts on the corneodesmosome protein binding junctions within the tissue. CONCLUSIONS:These results suggest that lipid loss causes marked increases in SC drying stresses, which may in turn contribute to changes in skin perception. NMF extraction may be important in vivo, but has remarkably little impact in isolated SC.
journal_name
Skin Pharmacol Physioljournal_title
Skin pharmacology and physiologyauthors
Bow JR,Sonoki Y,Uchiyama M,Shimizu E,Tanaka K,Dauskardt RHdoi
10.1159/000507456subject
Has Abstractpub_date
2020-01-01 00:00:00pages
180-188issue
4eissn
1660-5527issn
1660-5535pii
000507456journal_volume
33pub_type
杂志文章abstract：BACKGROUND/AIMS:The aim of the study was to elucidate the effect of sample preparation and microscope configuration on the results of confocal Raman microspectroscopic evaluation of the penetration of a pharmaceutical active into the skin (depth profiling). METHODS:Pig ear skin and a hydrophilic formulation containing...
journal_title：Skin pharmacology and physiology
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journal_title：Skin pharmacology and physiology
pub_type： 杂志文章,多中心研究,随机对照试验
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journal_title：Skin pharmacology and physiology
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journal_title：Skin pharmacology and physiology
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journal_title：Skin pharmacology and physiology
pub_type： 临床试验,杂志文章
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journal_title：Skin pharmacology and physiology
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journal_title：Skin pharmacology and physiology
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journal_title：Skin pharmacology and physiology
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journal_title：Skin pharmacology and physiology
pub_type： 杂志文章
doi：10.1159/000464471
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journal_title：Skin pharmacology and physiology
pub_type： 杂志文章
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更新日期：2004-09-01 00:00:00
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journal_title：Skin pharmacology and physiology
pub_type： 杂志文章,随机对照试验
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journal_title：Skin pharmacology and physiology
pub_type： 杂志文章
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journal_title：Skin pharmacology and physiology
pub_type： 杂志文章,随机对照试验
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更新日期：2018-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2009-01-01 00:00:00
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journal_title：Skin pharmacology and physiology
pub_type： 杂志文章,评审
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更新日期：2006-01-01 00:00:00
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journal_title：Skin pharmacology and physiology
pub_type： 杂志文章
doi：10.1159/000148042
更新日期：2008-01-01 00:00:00
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journal_title：Skin pharmacology and physiology
pub_type： 杂志文章
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pub_type： 临床试验,杂志文章
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journal_title：Skin pharmacology and physiology
pub_type： 杂志文章,评审
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journal_title：Skin pharmacology and physiology
pub_type： 杂志文章
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更新日期：2011-01-01 00:00:00
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