Codon and Propeptide Optimizations to Improve the Food-grade Expression of Bile Salt Hydrolase in Lactococcus lactis.

abstract：:Increasing antibiotic resistance has led to an urgent need for new therapeutic approaches. Host defense peptides are known to be antimicrobial and have revealed broad immunomodulatory functions for both innate and adaptive immunity. This review will focus on the role of host defense peptides in infection and immune re...

journal_title：Protein and peptide letters

authors： Hirsch T,Jacobsen F,Steinau HU,Steinstraesser L

更新日期：2008-01-01 00:00:00

abstract：:Transgelin was solubly expressed in E. coli. Crystals of transgelin have been grown at 291K using sodium formate or PEG-4000 as precipitants. X-ray diffraction by the crystal extends to 2.3 A resolution. The crystal belongs to the space group P2(1), with the unit cell parameters a=39.3, b=61.9, c=56.0 A and beta=90.2 ...

journal_title：Protein and peptide letters

authors： Liao X,Li S,Lou Z,Chen Z,Bartlam M

更新日期：2007-01-01 00:00:00

abstract：:Like those in mammals, heterotrimeric G protein complexes have been implicated in signal transduction pathways in plants; however, the subunits themselves have not been isolated. In this study, the rice heterotrimeric G protein subunits α (Gα) and β (Gβ) were purified by affinity chromatography using anti-Gα and -Gβ a...

journal_title：Protein and peptide letters

authors： Abe Y,Matsusita K,Komatsu S,Iwasaki Y

更新日期：2012-03-01 00:00:00

abstract：:Background. Obesity is a major health problem in both developed and emerging countries. Obesity is a complex disease whose etiology involves genetic factors in strong interplay with environmental determinants and lifestyle. The discovery of genetic factors and biological pathways underlying human obesity is hampered b...

journal_title：Protein and peptide letters

authors： Martelli PL,Fontanesi L,Piovesan D,Fariselli P,Casadio R

更新日期：2014-01-01 00:00:00

abstract：:Protein phosphatase magnesium-dependent 1, delta (PPM1D) is a member of the PPM1 (formerly PP2C) protein phosphatase family, and is induced in response to DNA damage. The overexpression of PPM1D is thought to exert oncogenic effects through the inhibition of tumor suppressor proteins. PPM1D shows high selectivity for ...

journal_title：Protein and peptide letters

authors： Chuman Y,Yagi H,Fukuda T,Nomura T,Matsukizono M,Shimohigashi Y,Sakaguchi K

更新日期：2008-01-01 00:00:00

abstract：:The self-assembly of short peptide building blocks into well-ordered nanostructures is a key direction in bionanotechnology. The formation of β -sheet organizations by short peptides is well explored, leading to the development of a wide range of functional assemblies. Likewise, many natural proteinaceous materials, s...

journal_title：Protein and peptide letters

authors： Bera S,Gazit E

更新日期：2019-01-01 00:00:00

abstract：:Porins are integral membrane proteins found in the outer membrane of bacteria, mitochondria and chloroplasts. Herein, we have reviewed sequence and structural understanding about bacterial porins. The first porin structure from Rhodobacter capsulatus at 1.8 Å resolution in 1991 till the recent structural advancement, ...

journal_title：Protein and peptide letters

authors： Kumar A,Bhandari A,Krishnaswamy S

更新日期：2015-01-01 00:00:00

abstract：:The immunoglobulin fold (Ig-fold) is a widespread structural motif that is detected in a variety of proteins involved in diversified biological processes. The Ig-fold contains 70-110 residues that are assembled in a characteristic sandwich-like structure formed by two facing β-sheets each made of antiparallel β-strand...

journal_title：Protein and peptide letters

authors： Berisio R,Ciccarelli L,Squeglia F,De Simone A,Vitagliano L

更新日期：2012-10-01 00:00:00

abstract：:Vasostatin has previously been expressed in fused form or in inclusion body form in Escherichia coli. Here the protein was expressed in soluble non-fusion form in BL21(DE3)pLysS by IPTG induction. The expression level of vasostatin was about 15% of the total cellular protein. The expressed vasostatin was purified and ...

journal_title：Protein and peptide letters

authors： Wu X,Li X,Su Z,Zheng Q,Xu H,Wu S,Feng Y,Zhao W

更新日期：2005-10-01 00:00:00

abstract：:A halophilic nucleoside diphosphate kinase from a moderate halophile, Halomonas sp. 593 (593NDK), was found to be resistant to heat treatment, as indicated by the high level of activity recovery after heating at high temperatures. This is due to reversibility of thermal unfolding, not the high melting temperature, of ...

journal_title：Protein and peptide letters

authors： Tokunaga H,Oda Y,Yonezawa Y,Arakawa T,Tokunaga M

更新日期：2006-01-01 00:00:00

abstract：:Spermadhesins are a family of secretory proteins expressed in the male genital tract of pig, horse and bull. Their function and structure have been widely studied, especially those isolated from boar. However, there are no data concerning spermadhesins isolated from buck. Buck seminal plasma was collected and subjecte...

journal_title：Protein and peptide letters

authors： Teixeira DI,Cavada BS,Sampaio AH,Havt A,Bloch C Jr,Prates MV,Moreno FB,Santos EA,Gadelha CA,Gadelha TS,Crisóstomo FS,Freitas VJ

更新日期：2002-08-01 00:00:00

abstract：BACKGROUND:Nε-acetyl L-α lysine is an unusual acetylated di-amino acid synthesized and accumulated by certain halophiles under osmotic stress. Osmolytes are generally known to protect proteins and other cellular components under various stress conditions. OBJECTIVE:The structural and functional stability imparted by N...

journal_title：Protein and peptide letters

authors： Joghee NN,Jayaraman G,Selladurai M

更新日期：2020-01-01 00:00:00

abstract：:In eukaryotic cells, regulated protein degradation of intracellular proteins is mediated largely by the ubiquitin proteasome system (UPS). UPS-mediated protein degradation regulates virtually all crucial aspects of cellular physiology, such as cell proliferation, cell division, cell differentiation, and cell death. Co...

journal_title：Protein and peptide letters

authors： Eldeeb M,Fahlman R

更新日期：2016-01-01 00:00:00

abstract：:Allergy prevalence has increased worldwide over the last 25 years along with industrialization and westernized lifestyles. Indoor allergens are primarily responsible for the sensitization and development of atopic diseases. The main indoor allergens are known to be derived from various arthropods which account for up ...

journal_title：Protein and peptide letters

authors： Jeong KY,Hong CS,Yong TS

更新日期：2007-01-01 00:00:00

abstract：:Michael Laskowski Jr. (1930-2004) was a pioneer in the field of Standard mechanism serine proteinase inhibitors. He made numerous important contributions in the field. This article highlights some of his most important contributions such as the discovery of the reactive site in serine proteinase inhibitors, the propos...

journal_title：Protein and peptide letters

authors： Qasim MA

更新日期：2005-07-01 00:00:00

abstract：:A pivotal feature that underlies the development of neurodegeneration is the accumulation of protein aggregates. In response, eukaryotic cells have evolved sophisticated quality control mechanisms to identify, repair and/or eliminate the misfolded abnormal proteins. Chaperones identify any otherwise abnormal conformat...

journal_title：Protein and peptide letters

authors： Eldeeb MA,Ragheb MA,Esmaili M,Hussein F

更新日期：2020-01-01 00:00:00

abstract：:S-adenosyl-l-homocysteine hydrolase from a malaria parasite Plasmodium falciparum (PfSAHH) has been crystallized by the vapor diffusion method. The crystals belong to an orthorhombic space group P212121 with the cell dimensions of a = 76.66 A, b = 86.31 A, and c = 335.6 A. There are four subunits (one tetramer) per as...

journal_title：Protein and peptide letters

authors： Tanaka N,Kusakabe Y,Shiraiwa K,Sakamoto Y,Nakanishi M,Kitade Y,Nakamura KT

更新日期：2004-04-01 00:00:00

abstract：:We have studied the effect of guanidinium chloride (Gdn.Cl) and different osmolytes such as betaine, trimethylamine-N-oxide (TMAO) and urea on the rate of chymotrypsin catalyzed reaction. The rates were measured using three synthetic chromogenic substrates, succinyl-ala-ala-pro-arg-pNA (AAPR), succinyl-ala-ala-pro-leu...

journal_title：Protein and peptide letters

authors： Garringer M,Qasim MA

更新日期：2016-01-01 00:00:00

abstract：BACKGROUND:NMAAP1 plays a role in regulating macrophage differentiation to the M1 type and exerting antitumoral functions. It is not clear what role and mechanism NMAAP1 does play in the reversal of macrophages from M1 to M2. METHODS:We detected the typing of macrophages with high or low expression of NMAAP1 by QPCR a...

journal_title：Protein and peptide letters

authors： Liu Q,Zhu P,Liu S,Tang M,Wang Y,Tian Y,Jin Z,Li D,Yan D

更新日期：2019-01-01 00:00:00

abstract：:We present two efficient network propagation algorithms that operate on a binary tree, i.e., a sparse-edged substitute of an entire similarity network. TreeProp-N is based on passing increments between nodes while TreeProp-E employs propagation to the edges of the tree. Both algorithms improve protein classification e...

journal_title：Protein and peptide letters

authors： Kocsor A,Busa-Fekete R,Pongor S

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:Ciprofloxacin (Cip) is the most commonly used quinolone in clinical practice; however large-scale use has favored the increase of multiresistant pathogenic microorganisms. Antimicrobial peptides (AMPs) appear to be a promising alternative in potentiating these conventional drugs. OBJECTIVE:The aim of this s...

journal_title：Protein and peptide letters

authors： Carneiro VA,de Oliveira ST,Silva RL,de Sousa Duarte H,Silva ML,Matos MNC,Cavalcante RMB,Figueira CS,Lorenzón EN,Cilli EM,da Cunha RMS

更新日期：2020-01-01 00:00:00

abstract：:Cinnamomin is a type II ribosome-inactivating protein (RIP) and its A-chain (CTA) is a RNA N-glycosidase. It is observed that modification of tyrosine residues by N-acetylimidazole (N-AI) causes almost complete loss of CTA activity. Adenine partially protects tyrosine residues from modification by N-AI. It is proposed...

journal_title：Protein and peptide letters

authors： Xu H,Liu WY

更新日期：2003-10-01 00:00:00

abstract：:ROS, including (.)OH, is now recognized as the hallmark of inflammation and damage to the anti-proteinase barrier has been implicated in a number of pathophysiological conditions. We have previously shown that O2( -) and H2O2/HOCl are physiologically relevant inactivators of alpha 2M, a key anti-proteinase. Here, we s...

journal_title：Protein and peptide letters

authors： Khan SA,Khan FH

更新日期：2009-01-01 00:00:00

abstract：:A series of 30 tripeptides were synthesized and tested as novel 5-HT4 receptor ligands. Receptor binding assays showed that a subset of compounds had reasonable potency relative to the agonists serotonin and 5-methoxytryptamine. Structure-activity analyses and molecular docking have highlighted avenues for further syn...

journal_title：Protein and peptide letters

authors： Hanna-Elias A,Thompson PE,Manallack DT,Irving HR,Coupar IM,Berque-Bestel I,Iskander MN

更新日期：2009-01-01 00:00:00

abstract：:This study revealed that the content of protein S29 in ribosomes of cancer cell line A549 was distinctly low (equivalent to about 30% of that of 2BS). The conclusion was acquired based on the ratios of spot volume of ribosomal protein S29 to that of several other ribosomal proteins (S29/L37a, S29/L38, S29/S27 and S29/...

journal_title：Protein and peptide letters

authors： Zhou ZD,Bao L,Liu DG,Li MQ,Ge YZ,Huang YL,Liu WY

更新日期：2003-02-01 00:00:00

abstract：:An alternative method for expressing the recombinant proteins in Escherichia coli is proposed. Unlike the ordinary induction protocol where the cells in the early- to mid-log phase are induced for the protein production, this alternative protocol utilizes the cells in the stationary phase. By using a glutathione S-tra...

journal_title：Protein and peptide letters

authors： Chae YK,Cho KS,Chun W,Lee K

更新日期：2003-08-01 00:00:00

abstract：:Bromelain inhibitor, "bromein", is a proteinase-inhibitor specific to the cysteine proteinase bromelain from pineapple stem. In the stem, eight bromein isoforms are known to exist, and each isoform has a short peptide (light chain) and a long one (heavy chain) with five disulfide bonds. The three-dimensional structure...

journal_title：Protein and peptide letters

authors： Hatano KI,Takahashi K,Tanokura M

更新日期：2018-01-01 00:00:00

abstract：:With a huge amount of protein sequence data, the computational method for protein-protein interaction (PPI) prediction using only the protein sequences information have drawn increasing interest. In this article, we propose a sequence-based method based on a novel representation of local protein sequence descriptors. ...

journal_title：Protein and peptide letters

authors： Yang L,Xia JF,Gui J

更新日期：2010-09-01 00:00:00

abstract：:Nucleobindin-2 is a 420 amino acid EF-hand Ca²⁺ binding protein that can be further processed to generate an 82 amino terminal peptide termed Nesfatin-1. To examine the function of secreted Nucleobindin-2 in adipocyte differentiation, cultured 3T3-L1 cells were incubated with either 0 or 100 nM of GST, GST-Nucleobindi...

journal_title：Protein and peptide letters

authors： Tagaya Y,Osaki A,Miura A,Okada S,Ohshima K,Hashimoto K,Yamada M,Satoh T,Shimizu H,Mori M

更新日期：2012-09-01 00:00:00

abstract：BACKGROUND:It is currently believed that protein folding rates are influenced by protein structure, environment and temperature, amino acid sequence and so on. We have been working for long to determine whether and in what ways mRNA affects the protein folding rate. A large number of palindromes aroused our attention i...

journal_title：Protein and peptide letters

authors： Li R,Li H,Yang S,Feng X

更新日期：2020-01-01 00:00:00