Abstract:
:Spermadhesins are a family of secretory proteins expressed in the male genital tract of pig, horse and bull. Their function and structure have been widely studied, especially those isolated from boar. However, there are no data concerning spermadhesins isolated from buck. Buck seminal plasma was collected and subjected to ion exchange chromatography on DEAE-Sephacel column followed by chromatography in a C18 column coupled to a HPLC system. The purification of the protein was determined by SDS-PAGE and MALDI-TOF analysis exhibiting a molecular mass of 12.5 KDa and showed to be structurally homologous to spermadhesins from boar and stallion.
journal_name
Protein Pept Lettjournal_title
Protein and peptide lettersauthors
Teixeira DI,Cavada BS,Sampaio AH,Havt A,Bloch C Jr,Prates MV,Moreno FB,Santos EA,Gadelha CA,Gadelha TS,Crisóstomo FS,Freitas VJdoi
10.2174/0929866023408670subject
Has Abstractpub_date
2002-08-01 00:00:00pages
331-5issue
4eissn
0929-8665issn
1875-5305journal_volume
9pub_type
杂志文章abstract:BACKGROUND:Multiple Sequence Alignment (MSA) is a fundamental task in bioinformatics and is required for many biological analysis tasks. The more accurate the alignments are, the more credible the downstream analyses. Most protein MSA algorithms realign an alignment to refine it by dividing it into two groups horizonta...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866526666190806143959
更新日期:2020-01-01 00:00:00
abstract::Current advancements in biological protein discovery utilize bi-partite methods of fluorescence detection where chromophore and scaffold are uncoupled. One such technology, called fluorogen-activating proteins (FAPs), consists of single-chain-variable-fragments (scFvs) selected against small organic molecules (fluorog...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
abstract::There is accumulating evidence that proteinuria is not merely a biomarker for the progression of chronic kidney disease (CKD), but also a mediator of this devastating disorder. Indeed, albumin, one of the major components found in proteinuria, causes proinflammatory and profibrotic changes in cultured proximal tubular...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986610789909430
更新日期:2010-01-01 00:00:00
abstract::DNA ligase is an important enzyme and it plays vital role in the replication and repair; also catalyzes nick joining between adjacent bases of DNA. The NAD(+) dependent DNA ligase is selectively present in eubacteria and few viruses; but missing in humans. Homology modeling was used to generate 3-D structure of NAD(+)...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986610790225950
更新日期:2010-02-01 00:00:00
abstract::We used a de novo designed, beta-hairpin forming T1 peptide as a model to investigate the kinetics of peptide fibrogenesis by a combination of light scattering (LS), circular dichroism (CD), fluorescence, and atomic force microscopy (AFM). The results demonstrate that the T1 fibrogenesis undergoes a consecutive stepwi...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986610790963690
更新日期:2010-04-01 00:00:00
abstract::The aerotolerance of the lactic - fermentative bacterium Streptococcus thermophilus is mainly based on the key antioxidant function of superoxide dismutase (StSOD). In this work, the comparison of recombinant StSOD (rStSOD) forms obtained from two different initiation triplets indicated that the enzyme from S. thermop...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986612799363127
更新日期:2012-03-01 00:00:00
abstract::Quantum mechanical fragment molecular orbital calculations have been performed for receptor binding of the hemagglutinin protein of the recently pandemic influenza 2009 H1N1, A/swine/Iowa/1930, and A/Puerto Rico/8/1934 viruses to α2-6 linked sialyloligosaccharides, as analogs of human receptors. The strongest receptor...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986611794927893
更新日期:2011-05-01 00:00:00
abstract:BACKGROUND:Protein-carbohydrate interactions play vital roles in several biological processes in living organisms. The comparative analysis of binding site residues along with stabilizing residues in protein-carbohydrate complexes provides ample insights to understand the structure, function and recognition mechanism. ...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866525666180221122529
更新日期:2018-01-01 00:00:00
abstract::Michael Laskowski Jr. (1930-2004) was a pioneer in the field of Standard mechanism serine proteinase inhibitors. He made numerous important contributions in the field. This article highlights some of his most important contributions such as the discovery of the reactive site in serine proteinase inhibitors, the propos...
journal_title:Protein and peptide letters
pub_type: 传,历史文章,杂志文章
doi:10.2174/0929866054395347
更新日期:2005-07-01 00:00:00
abstract:BACKGROUND:Laminin, a member of the Extracellular Matrix (ECM), is a glycoprotein that is used as a factor that affects cell adhesion, proliferation, survival, and differentiation. Of these, five globular domains (LG domains) of the alpha chain play an important role in influencing the cell by binding to the integrin. ...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866526666190617091155
更新日期:2019-01-01 00:00:00
abstract::We report the stepwise transformation of a linear peptide epitope recognized by the anti-transforming growth factor alpha monoclonal antibody Tab2 into peptomers and finally into peptoid analogs. The key experiment in this study is the substitution analysis in which each position of the peptide is exchanged by a set o...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986606777841299
更新日期:2006-01-01 00:00:00
abstract::Hepatitis C virus (HCV) triggers coordinated innate and adaptive response in host cell. HCV genome and proteins of the replicating virus are recognized as non-self-antigens by host cell to activate Toll Like Receptors (TLRs). Activated TLRs ultimately express cytokines, which can clear virus either by activating inter...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866523666160419150136
更新日期:2016-01-01 00:00:00
abstract::For biophysical studies using heteronuclear NMR analysis of amyloid beta peptide, construction of an efficient and high yield expression system of uniformly isotopic labeled amyloid beta peptide is desirable. Here we succeeded in obtaining (15)N-labeled amyloid beta 1-40 expressed by attachment to hen egg white lysozy...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986607781483741
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND:Hepatitis C Virus (HCV) is a major cause of chronic liver disease in the world. Many studies showed that T-cell responses to HCV Nonstructural Protein 3 (NS3) play an important role in clearing acute HCV infections, thus NS3 can be considered as a suitable candidate antigen for development of a HCV therapeut...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866525666180925145304
更新日期:2018-01-01 00:00:00
abstract::Prediction of protein structure from its amino acid sequence is still a challenging problem. The complete physicochemical understanding of protein folding is essential for the accurate structure prediction. Knowledge of residue solvent accessibility gives useful insights into protein structure prediction and function ...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986612798472875
更新日期:2012-01-01 00:00:00
abstract::We report the physical-chemical characterization of the major alcohol-soluble proteins present in seeds of pearl millet (Pennisetum glaucum) by SDS-PAGE, bidimensional gel electrophoresis, MALDI-TOF/MS and RP-HPLC. We demonstrate the presence of three major prolamins, called A-, B- and C-pennisetin with mass values ar...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866023408724
更新日期:2002-06-01 00:00:00
abstract::Though protein folding is a regular phenomenon inside the cellular milieu, slight differences in the folding pattern of proteins may lead to disease-causing forms. Many diseases have been identified that are caused by these misfolded macromolecules and a considerable amount of focus is still directed towards better un...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:10.2174/0929866043406409
更新日期:2004-12-01 00:00:00
abstract::Human histatins are histidine-rich, low molecular weight salivary proteins that contribute to the immune system of the oral cavity. In this work, nucleotide sequences of the HIS1 (coding for histatin 1) and HIS2 (coding for histatin 3) genes, homologous to the human ones, have been sequenced and analysed in five prima...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986610791306715
更新日期:2010-07-01 00:00:00
abstract::This study revealed that the content of protein S29 in ribosomes of cancer cell line A549 was distinctly low (equivalent to about 30% of that of 2BS). The conclusion was acquired based on the ratios of spot volume of ribosomal protein S29 to that of several other ribosomal proteins (S29/L37a, S29/L38, S29/S27 and S29/...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866033408273
更新日期:2003-02-01 00:00:00
abstract::Sry-related box (Sox) transcription factors share a conserved high-mobility-group box domain (HMG-domain) that binds DNA in the minor groove and bends DNA for further assembly of transcriptional machineries. During organogenesis, each member of the Sox family triggers a specific cell lineage differentiation, indicatin...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:
更新日期:2013-04-01 00:00:00
abstract::Comparison between the BAD complexes indicated that BAD all docks a hydrophobic surface of PKAc regardless of its phosphorylation. PKAc may prevent Bcl-xL from rebinding to BAD by phosphorylating human BAD at Ser118; whereas human BAD is phosphorylated on Ser75 in a BAD-Bcl-xL complex, resulting in the dissociation of...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986610790226003
更新日期:2010-02-01 00:00:00
abstract:BACKGROUND:In the last years, Antimicrobial Peptides (AMPs) and lipopeptides have received attention as promising candidates to treat infections caused by resistant microorganisms. OBJECTIVE:The main objective of this study was to investigate the effect of repetitive KLFK motifs and the attachment of aliphatic acids t...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866526666181208144629
更新日期:2019-01-01 00:00:00
abstract::Polymyositis is an inflammatory myopathy characterized by muscle invasion of T-cells penetrating the basal lamina and displacing the plasma membrane of normal muscle fibers. In order to understand the different adhesive mechanisms at the T-cell surface, Schubert randomly selected 19 proteins expressed at the T-cell su...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986612801619534
更新日期:2012-08-01 00:00:00
abstract::Tumor vasculatures express high levels of α(V)β(3)/α(V)β(5) and α(5)β(1) integrins. Peptide containing the RGD (Arg-Gly-Asp) sequence, which is present in ligands of integrins, is effective in targeting therapeutic reagents to tumor vascular endothelium. In this study, we investigated whether the biological activity o...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:
更新日期:2011-06-15 00:00:00
abstract::The self-assembly of short peptide building blocks into well-ordered nanostructures is a key direction in bionanotechnology. The formation of β -sheet organizations by short peptides is well explored, leading to the development of a wide range of functional assemblies. Likewise, many natural proteinaceous materials, s...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:10.2174/0929866525666180917163142
更新日期:2019-01-01 00:00:00
abstract::The immunoglobulin fold (Ig-fold) is a widespread structural motif that is detected in a variety of proteins involved in diversified biological processes. The Ig-fold contains 70-110 residues that are assembled in a characteristic sandwich-like structure formed by two facing β-sheets each made of antiparallel β-strand...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:10.2174/092986612802762732
更新日期:2012-10-01 00:00:00
abstract::An efficient preparation of Periplaneta americana nymphae allergen, Cr PI (54 kDa) is described. It was expressed as a GST-tag fusion protein in Escherichia coli, strain BL21 (DE3). Expression of recombinant Cr PI (rCr PI), denaturation/renaturation of the inclusion bodies and the effects of protein and L-arginine con...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986607782110266
更新日期:2007-01-01 00:00:00
abstract::In general, halophilic proteins are stable only in the presence of salts at high concentrations. Not only is high salt concentration important for structural stability of halophilic proteins, but also refolding of a denatured halophilic protein requires high salt concentration. This review summarizes the importance of...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:10.2174/0929866043478220
更新日期:2004-04-01 00:00:00
abstract::E. coli small heat shock proteins IbpA and IbpB (inclusion body binding proteins A and B) are known to act as holding chaperones on denaturing, aggregate-prone proteins. But, there is no clear understanding about which of the IbpA and IbpB has more holdase activity and how the holdase activity of one was influenced by...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866521666131224094408
更新日期:2014-06-01 00:00:00
abstract::Loxosceles intermedia spider venom was subjected to proteomic analysis through a MudPIT shot-gun approach to identify the protein composition. Were identified 39 proteins which seem to responsible by the lesion of different types of tissues, to some physiopathological actions and by the prevention of structural damage...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986609788923383
更新日期:2009-01-01 00:00:00