Abstract：
:Gene modifications in the PmrAB and PhoPQ two-component regulatory systems, as well as inactivation of the mgrB gene, are known to be causes of colistin resistance in Klebsiella pneumoniae. The objective of this study was to characterise the mechanism involved in colistin resistance in a Klebsiella oxytoca isolate. A K. oxytoca clinical isolate showing resistance to colistin was recovered in Cali, Colombia. The pmrA, pmrB, phoP, phoQ and mgrB genes were amplified and sequenced. Wild-type mgrB genes from K. pneumoniae and K. oxytoca were cloned, and corresponding recombinant plasmids were used for complementation assays. By analysing the mgrB gene of the K. oxytoca isolate and its flanking sequences, an insertion sequence (IS) of 1196bp was identified in its promoter region. The insertion was located between nucleotides -39 and -38 when referring to the start codon of the mgrB gene, thus negatively interfering with expression of the mgrB gene by modifying its promoter structure. This IS was very similar to ISKpn26 (99% nucleotide identity) belonging to the IS5 family. Complementation assays with mgrB genes from wild-type K. pneumoniae or K. oxytoca restored full susceptibility to colistin. In conclusion, here we identified the mechanism involved in colistin resistance in a K. oxytoca isolate. Modulation of mgrB gene expression was the key factor for this acquired resistance to colistin.
journal_name
Int J Antimicrob Agentsjournal_title
International journal of antimicrobial agentsauthors
Jayol A,Poirel L,Villegas MV,Nordmann Pdoi
10.1016/j.ijantimicag.2015.02.015subject
Has Abstractpub_date
2015-07-01 00:00:00pages
108-10issue
1eissn
0924-8579issn
1872-7913pii
S0924-8579(15)00109-0journal_volume
46pub_type
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journal_title：International journal of antimicrobial agents
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journal_title：International journal of antimicrobial agents
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journal_title：International journal of antimicrobial agents
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journal_title：International journal of antimicrobial agents
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journal_title：International journal of antimicrobial agents
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