Abstract:
:S fimbrial adhesins (Sfa) represent virulence factors of E. coli wild-type strains causing urinary tract infections and meningitis of the new born. In order to determine the influence of subinhibitory concentration of antibiotics on the expression of the sfa gene cluster, a wild-type strain carrying the lacZ gene, coding for the enzyme beta-galactosidase fused to the sfa determinant was used. The expression of lacZ which was under the control of the sfa wild-type promoters, was now equivalent to the sfa gene expression of wild-type strain 536. With this strain the influence of subinhibitory concentrations of 28 antibiotics on the expression of the sfa determinant was studied. The expression was strongly suppressed by a treatment of the wild-type fusion strain by aztreonam, gentamicin, clindamycin and trimethoprim; the latter had a dramatic effect on sfa expression. It was further shown for clindamycin and trimethoprim that the reduction of sfa gene expression was dependent on the concentration of the antibiotics. In contrast imipinem, amphotericin B and rifampicin weakly stimulated sfa expression. We conclude that gene fusions between virulence-associated loci and indicator genes in wild-type pathogens are useful to study virulence modulation due to subinhibitory concentration of antibiotics on the genetic level.
journal_name
Int J Antimicrob Agentsjournal_title
International journal of antimicrobial agentsauthors
Hacker J,Ott M,Hof Hdoi
10.1016/0924-8579(93)90060-isubject
Has Abstractpub_date
1993-01-01 00:00:00pages
263-70issue
4eissn
0924-8579issn
1872-7913pii
0924-8579(93)90060-Ijournal_volume
2pub_type
杂志文章abstract::Integrase strand transfer inhibitors (INSTIs) are the most recent class of antiretroviral drugs with potent and durable antiviral activity used to treat human immunodeficiency virus type 1 (HIV-1) infection. However, development of drug resistance increases the risk of treatment failure, disease progression and mortal...
journal_title:International journal of antimicrobial agents
pub_type: 杂志文章,meta分析,评审
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更新日期:2019-11-01 00:00:00
abstract::In vitro and in vivo anti-Candida albicans and anti-Aspergillus fumigatus activities of NND-502, a new imidazole-antimycotic, were compared with those of fluconazole (FCZ), itraconazole (ITZ) and/or amphotericin B (AmB). NND-502 exhibited strong in vitro antifungal activity against both fungal species; its MIC against...
journal_title:International journal of antimicrobial agents
pub_type: 杂志文章
doi:10.1016/s0924-8579(99)00076-x
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abstract::Leptospirosis is the most common zoonotic disease and is endemic worldwide. The antibiotic susceptibilities of Leptospira strains isolated from both humans and animals are poorly documented. This issue is particularly important for isolates from food-producing animals which are regularly exposed to antibiotic treatmen...
journal_title:International journal of antimicrobial agents
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abstract::Infestation with parasitic helminths is a common problem in human populations of third world countries and is ubiquitous in livestock and other domestic animals. The cell-membrane efflux pump, P-glycoprotein (Pgp), appears to contribute to anthelmintic resistance. Pgp have been identified from both phyla of parasitic ...
journal_title:International journal of antimicrobial agents
pub_type: 杂志文章,评审
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abstract::Based on past information from the literature and our own review, it appears that imipenem can be used effectively as an initial empirical therapy of febrile neutropenia, as a monotherapy, even in patients with haematological malignancies. The response rate is outstanding in microbiologically documented infections, na...
journal_title:International journal of antimicrobial agents
pub_type: 杂志文章,评审
doi:10.1016/s0924-8579(03)00002-5
更新日期:2003-05-01 00:00:00
abstract::Invasive aspergillosis of the central nervous system has a mortality rate exceeding 90%. We describe a 29-year-old woman with a medical history of chronic polyarthritis who developed a proven rhinocerebral Aspergillus fumigatus infection refractory to first-line treatment with liposomal amphotericin B. The patient res...
journal_title:International journal of antimicrobial agents
pub_type: 杂志文章
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更新日期:2006-09-01 00:00:00
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journal_title:International journal of antimicrobial agents
pub_type: 杂志文章,评审
doi:10.1016/s0924-8579(01)00406-x
更新日期:2001-01-01 00:00:00
abstract::The aim of this study was to determine the clinical characteristics, molecular epidemiology and biofilm production of Acinetobacter baumannii clinical isolates obtained from a tertiary-care hospital in Mexico. Clinical isolates of A. baumannii (n=152) isolated from 2007 to 2012 were included. Clonal diversity was anal...
journal_title:International journal of antimicrobial agents
pub_type: 杂志文章
doi:10.1016/j.ijantimicag.2014.10.022
更新日期:2015-03-01 00:00:00
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journal_title:International journal of antimicrobial agents
pub_type: 杂志文章
doi:10.1016/j.ijantimicag.2006.11.032
更新日期:2007-06-01 00:00:00
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journal_title:International journal of antimicrobial agents
pub_type: 杂志文章
doi:10.1016/j.ijantimicag.2013.06.019
更新日期:2013-10-01 00:00:00
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journal_title:International journal of antimicrobial agents
pub_type: 杂志文章
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更新日期:2016-04-01 00:00:00
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journal_title:International journal of antimicrobial agents
pub_type: 杂志文章
doi:10.1016/j.ijantimicag.2013.08.016
更新日期:2014-01-01 00:00:00
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journal_title:International journal of antimicrobial agents
pub_type: 杂志文章
doi:10.1016/j.ijantimicag.2010.08.008
更新日期:2011-01-01 00:00:00
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journal_title:International journal of antimicrobial agents
pub_type: 杂志文章
doi:10.1016/j.ijantimicag.2010.10.021
更新日期:2011-02-01 00:00:00
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journal_title:International journal of antimicrobial agents
pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:1999-09-01 00:00:00
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journal_title:International journal of antimicrobial agents
pub_type: 杂志文章
doi:10.1016/j.ijantimicag.2006.09.025
更新日期:2007-03-01 00:00:00
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journal_title:International journal of antimicrobial agents
pub_type: 杂志文章
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更新日期:1996-04-01 00:00:00
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journal_title:International journal of antimicrobial agents
pub_type: 杂志文章,评审
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更新日期:2010-11-01 00:00:00
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journal_title:International journal of antimicrobial agents
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2012-10-01 00:00:00
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journal_title:International journal of antimicrobial agents
pub_type: 杂志文章
doi:10.1016/j.ijantimicag.2017.01.023
更新日期:2017-06-01 00:00:00
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journal_title:International journal of antimicrobial agents
pub_type: 杂志文章
doi:10.1016/s0924-8579(03)00178-x
更新日期:2003-10-01 00:00:00
abstract::Nacubactam is a novel non-β-lactam diazabicyclooctane β-lactamase inhibitor under development for the treatment of serious Gram-negative infections. This study assessed the efficacy of human-simulated epithelial lining fluid (ELF) exposure of nacubactam in combination with meropenem against AmpC-overproducing (n=4) an...
journal_title:International journal of antimicrobial agents
pub_type: 杂志文章
doi:10.1016/j.ijantimicag.2019.10.019
更新日期:2020-02-01 00:00:00
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journal_title:International journal of antimicrobial agents
pub_type: 杂志文章
doi:10.1016/j.ijantimicag.2019.10.010
更新日期:2020-01-01 00:00:00
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journal_title:International journal of antimicrobial agents
pub_type: 杂志文章
doi:10.1016/j.ijantimicag.2016.12.008
更新日期:2017-04-01 00:00:00
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journal_title:International journal of antimicrobial agents
pub_type: 杂志文章
doi:10.1016/s0924-8579(02)00010-9
更新日期:2002-05-01 00:00:00
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journal_title:International journal of antimicrobial agents
pub_type: 杂志文章
doi:10.1016/j.ijantimicag.2015.06.013
更新日期:2015-10-01 00:00:00
abstract::Vancomycin is a glycopeptide antibiotic widely used in the management of meticillin-resistant Staphylococcus aureus (MRSA). Guidelines currently recommend vancomycin be administered by intermittent infusion, despite recent research suggesting that continuous infusion (CI) may be associated with lower rates of vancomyc...
journal_title:International journal of antimicrobial agents
pub_type: 杂志文章,meta分析,评审
doi:10.1016/j.ijantimicag.2015.04.013
更新日期:2015-09-01 00:00:00
abstract::To characterise the prevalence of β-lactamases and 16S rRNA methylase genes amongst clinical Klebsiella pneumoniae isolates carrying plasmid-mediated quinolone resistance (PMQR) determinants in China, 59 non-duplicate K. pneumoniae isolates harbouring at least one PMQR gene were screened for common β-lactamases and 16...
journal_title:International journal of antimicrobial agents
pub_type: 杂志文章
doi:10.1016/j.ijantimicag.2010.12.018
更新日期:2011-04-01 00:00:00
abstract::The purpose of this study was to characterise the metallo-beta-lactamase VIM-6 and the genetic environment of the bla(VIM-6) gene. The bla(VIM-6) gene was cloned into an Escherichia coli expression system and the purified VIM-6 enzyme was obtained. VIM-6 has an isoelectric point of 4.9 and a molecular weight of 28.368...
journal_title:International journal of antimicrobial agents
pub_type: 杂志文章
doi:10.1016/j.ijantimicag.2008.05.017
更新日期:2008-11-01 00:00:00