Abstract:
:We have shown that human B-cell non-Hodgkin lymphomas (B-NHLs) express heat shock protein (HSP)H1/105 in function of their aggressiveness. Here, we now clarify its role as a functional B-NHL target by testing the hypothesis that it promotes the stabilization of key lymphoma oncoproteins. HSPH1 silencing in 4 models of aggressive B-NHLs was paralleled by Bcl-6 and c-Myc downregulation. In vitro and in vivo analysis of HSPH1-silenced Namalwa cells showed that this effect was associated with a significant growth delay and the loss of tumorigenicity when 10(4) cells were injected into mice. Interestingly, we found that HSPH1 physically interacts with c-Myc and Bcl-6 in both Namalwa cells and primary aggressive B-NHLs. Accordingly, expression of HSPH1 and either c-Myc or Bcl-6 positively correlated in these diseases. Our study indicates that HSPH1 concurrently favors the expression of 2 key lymphoma oncoproteins, thus confirming its candidacy as a valuable therapeutic target of aggressive B-NHLs.
journal_name
Bloodjournal_title
Bloodauthors
Zappasodi R,Ruggiero G,Guarnotta C,Tortoreto M,Tringali C,Cavanè A,Cabras AD,Castagnoli L,Venerando B,Zaffaroni N,Gianni AM,De Braud F,Tripodo C,Pupa SM,Di Nicola Mdoi
10.1182/blood-2014-07-590034subject
Has Abstractpub_date
2015-03-12 00:00:00pages
1768-71issue
11eissn
0006-4971issn
1528-0020pii
blood-2014-07-590034journal_volume
125pub_type
杂志文章相关文献
BLOOD文献大全abstract::Hypercoagulability and thrombotic tendency are frequently induced by a variety of stressors. Clinically, aged subjects and obese patients are more susceptible to thrombotic diseases associated with stress, but the underlying mechanisms are unknown. We investigated the expression of a procoagulant gene, tissue factor (...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2002-03-0945
更新日期:2002-12-01 00:00:00
abstract::Duchenne muscular dystrophy (DMD) is caused by mutations in the dystrophin gene on the X-chromosome that result in skeletal and cardiac muscle damage and premature death. Studies in mice, including the mdx mouse model of DMD, have demonstrated that circulating bone marrow-derived cells can participate in skeletal musc...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2004-06-2247
更新日期:2004-12-15 00:00:00
abstract::A specially designed rotational viscometer was used to investigate the effects of the antiplatelet agent PGI2 in combination with theophylline on the response of human platelets subjected to shear stress. Samples of citrated platelet-rich plasma (PRP) were exposed to shear stress in the viscometer for a period of 5 mi...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1981-10-01 00:00:00
abstract::Human natural killer (NK) cell development is a step-by-step process characterized by phenotypically identified stages. CD161 is a marker informative of the NK cell lineage commitment, whereas CD56, CD117, and CD94/NKG2A contribute to define discrete differentiation stages. In cells undergoing in vitro differentiation...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-09-379693
更新日期:2012-04-26 00:00:00
abstract::A genetic mutation in mice (W/Wv) causes an autosomal recessive disease characterized by hypoplastic anemia which lasts throughout life. Double-dominant W/Wv anemic mice were sublethally irradiated to facilitate repopulation of marrow with transplanted cells and were injected intravenously with suspensions of 5-10 mil...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1977-10-01 00:00:00
abstract::As a mediator of neurogenic inflammation and pain, we hypothesized that levels of the neuropeptide Substance P (SP) would be elevated in patients with sickle cell disease (SCD) with vaso-occlusive pain crisis. SP is a known stimulator of tumor necrosis factor-alpha (TNF-alpha) release and a promoter of interleukin-8 (...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1998-11-01 00:00:00
abstract::We have previously reported that continuous intravenous (IV) administration of recombinant granulocyte-macrophage colony-stimulating factor (rHuGM-CSF) to humans following high-dose alkylating agent chemotherapy and autologous bone marrow support (ABMS) results in myeloid bone marrow maturation, accelerated granulocyt...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1988-10-01 00:00:00
abstract::In utero transplantation (IUT) is becoming a viable option for the treatment of various immune and metabolic disorders diagnosed early in gestation. In this study, donor fetal liver cells had a 10-fold competitive engraftment advantage relative to adult bone marrow in allogeneic fetal severe combined immunodeficient (...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v99.5.1870
更新日期:2002-03-01 00:00:00
abstract::The coagulation factors V (FV) and VIII (FVIII) are important at sites of vascular injury for the amplification of the clotting cascade. Natural variants of these factors frequently lead to severe bleeding disorders. To understand the mechanisms of activation of FVIII by thrombin, we used a bank of mutant thrombins to...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2002-03-0843
更新日期:2002-10-15 00:00:00
abstract::The c-Myb transcription factor controls differentiation and proliferation in hematopoietic and other cell types and has latent transforming activity, but little is known about its regulation during the cell cycle. Here, c-Myb was identified as part of a protein complex from human T cells containing the cyclin-dependen...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2004-08-3342
更新日期:2005-05-15 00:00:00
abstract::Erythroid cell iron and transferrin uptake and release was studied in the anemia of the Belgrade laboratory rat (gene symbol, b), an autosomal recessive trait characterized by hypochromia and hyperferrinemia. When reticulocyte-rich red cells were incubated in vitro with doubly (59Fe, 125I) labeled transferrin, b/b cel...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1980-04-01 00:00:00
abstract::The mevalonate pathway produces many critical substances in cells, including sterols essential for membrane structure and isoprenoids vital to the function of many membrane proteins. 3-Hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase is a rate-limiting enzyme in the mevalonate pathway. Because cholesterol is a ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2002-07-2283
更新日期:2003-05-01 00:00:00
abstract::A large number of alterations in genes encoding receptor tyrosine kinase (RTK), namely FLT3, c-KIT, platelet-derived growth factor (PDGF) receptors, fibroblast growth factor (FGF) receptors, and the anaplastic large cell lymphoma kinase (ALK), have been found in hematopoietic malignancies. They have drawn much attenti...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood-2010-04-279752
更新日期:2010-10-07 00:00:00
abstract::Preliminary reports have suggested that survivors of childhood cancer and aplastic anemia who are infected with the hepatitis C virus (HCV) have a low risk for progression to significant liver disease. Among our surviving patients who were transfused between 1961 and March 1992, 77 (6.6% of surviving patients tested t...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:2000-05-15 00:00:00
abstract::Imatinib mesylate (STI571), a potent tyrosine kinase inhibitor, is successfully used in the treatment of chronic myelogenous leukemia and gastrointestinal stromal tumors. However, the intended chronic oral administration of imatinib may lead to development of cellular resistance and subsequent treatment failure. Indee...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2004-04-1398
更新日期:2004-11-01 00:00:00
abstract::Heparin-induced thrombocytopenia (HIT) with thrombosis is a serious complication of heparin use. HIT sera can generate platelet-derived microparticles, which are produced in a heparin-dependent manner and are hypothesized to be important initial pathological participants because they promote vascular occlusion. To dat...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:2000-07-01 00:00:00
abstract::We tested baseline positron emission tomography (PET)/computed tomography (CT) as a measure of total tumor burden to better identify high-risk patients with early-stage Hodgkin lymphoma (HL). Patients with stage I-II HL enrolled in the standard arm (combined modality treatment) of the H10 trial (NCT00433433) with avai...
journal_title:Blood
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1182/blood-2017-07-795476
更新日期:2018-03-29 00:00:00
abstract::Activation of Janus tyrosine kinases (Jak) and Signal transducers and activators of transcription (Stat) after ligation of major histocompatibility complex class I (MHC-I) was explored in Jurkat T cells. Cross-linking of MHC-I mediated tyrosine phosphorylation of Tyk2, but not Jak1, Jak2, and Jak3. In addition, the tr...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1998-05-15 00:00:00
abstract::We recently demonstrated that immunoglobulin E (IgE), in the absence of cross-linking agents, activates signaling pathways in healthy murine bone marrow-derived mast cells (BMMCs) and that this activation enhances BMMC survival, at least in part, via secretion of autocrine-acting cytokines. We report herein that IgE a...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2002-10-3176
更新日期:2003-08-15 00:00:00
abstract::Although molecular biologic techniques can now detect minimal numbers of residual cancer cells in patients in complete clinical remission, the clinical significance of minimal residual disease has never been conclusively established. If the detection of minimal residual disease predicts which patients will relapse, th...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1993-06-15 00:00:00
abstract::The role of fibroblast growth factors (FGFs) in blood vessel formation has remained unclear. We used differentiating stem-cell cultures (embryoid bodies) and teratomas to show that FGF receptor-1 (FGFR-1) exerts a negative regulatory effect on endothelial cell function in these models. Embryoid bodies lacking expressi...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2007-01-067314
更新日期:2007-12-15 00:00:00
abstract::In the past 5 years we have witnessed significant advances in both the diagnostic process and optimal therapy for patients with essential thrombocythemia (ET). Insights into the underlying molecular mechanisms have been accompanied by the development of new diagnostic tests and by an improved understanding of the rela...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-08-270033
更新日期:2011-02-03 00:00:00
abstract::Chromosomal abnormalities in acute leukemia have led to the discovery of many genes involved in normal hematopoiesis and in malignant transformation. We have identified the fusion partners in an inv(8)(p11q13) from a patient with acute mixed lineage leukemia. We show by fluorescence in situ hybridization (FISH) analys...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1998-09-15 00:00:00
abstract::Interleukin-6 (IL-6) is a growth factor with diverse biologic activity. Originally described as a T-cell product that enhances immunoglobulin (Ig) secretion in antigen-stimulated B cells, it also affects the growth of T cells, plasmacytomas, hybridomas, and hematopoietic stem cells. We report the expression and secret...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1989-08-01 00:00:00
abstract::The development of neutralizing antibodies (inhibitors) against coagulation factor VIII (FVIII) is the most problematic and costly complication of FVIII replacement therapy that affects up to 30% of previously untreated patients with severe hemophilia A. The development of inhibitors is a multifactorial complication i...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2015-12-685735
更新日期:2016-06-09 00:00:00
abstract::The serine protease prolylcarboxypeptidase (PRCP), isolated from human umbilical vein endothelial cells (HUVECs), is a plasma prekallikrein (PK) activator. PRCP cDNA was cloned in pMT/BIP/V5-HIS-C, transfected into Schneider insect (S2) cells, and purified from serum-free media. Full-length recombinant PRCP (rPRCP) ac...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2003-07-2510
更新日期:2004-06-15 00:00:00
abstract::In mouse mid-gestational embryos, definitive hematopoietic stem progenitor cells are derived directly from a very small proportion of the arterial endothelium. However, the physiological mechanisms restraining excessive endothelial-hematopoietic transition remain elusive. We show here that genetic deletion of Smad4 fr...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2013-09-526053
更新日期:2014-04-03 00:00:00
abstract::In this issue of Blood, Baychelier et al identify a ligand for a major natural killer (NK) cell receptor that mediates natural cytotoxicity toward tumor cells, thus ending a search that lasted well over a decade. ...
journal_title:Blood
pub_type: 评论,杂志文章
doi:10.1182/blood-2013-09-523696
更新日期:2013-10-24 00:00:00
abstract::People with hemophilia were formerly at very high risk of infection with hepatitis C virus (HCV). Approximately 20% of HCV-infected patients spontaneously clear the virus. To identify correlates of spontaneous clearance of HCV, we studied a cohort of HCV-infected hemophilic subjects without human immunodeficiency viru...
journal_title:Blood
pub_type: 杂志文章,多中心研究
doi:10.1182/blood-2005-07-2781
更新日期:2006-02-01 00:00:00
abstract::Posttransplantation lymphoproliferative disorder (PTLD) is a devastating post-transplantation complication often associated with Epstein-Barr virus (EBV). Although the type and length of immunosuppression are risk factors, a patient's inherent immune capacity also likely contributes to this disorder. This report uses ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2003-07-2476
更新日期:2005-02-15 00:00:00