Abstract:
:Fronto-limbic circuits in the primate brain are responsible for executive function, learning and memory, and emotions, including fear. Consequently, changes in gene expression in cortical and subcortical brain regions housing these circuits are associated with many important psychiatric and neurological disorders. While high quality gene expression profiles can be identified in brains from model organisms, primate brains have unique features such as Brodmann Area 25, which is absent in rodents, yet profoundly important in primates, including humans. The potential insights to be gained from studying the human brain are complicated by the fact that the post-mortem interval (PMI) is variable, and most repositories keep solid tissue in the deep frozen state. Consequently, sampling the important medial and internal regions of these brains is difficult. Here we describe a novel method for obtaining discrete regions from the fronto-limbic circuits of a 4 year old and a 5 year old, male, intact, frozen non-human primate (NHP) brain, for which the PMI is exactly known. The method also preserves high quality RNA, from which we use transcriptional profiling and a new algorithm to identify region-exclusive RNA signatures for Area 25 (NFκB and dopamine receptor signaling), the anterior cingulate cortex (LXR/RXR signaling), the amygdala (semaphorin signaling), and the hippocampus (Ca(++) and retinoic acid signaling). The RNA signatures not only reflect function of the different regions, but also include highly expressed RNAs for which function is either poorly understood, or which generate proteins presently lacking annotated functions. We suggest that this new approach will provide a useful strategy for identifying changes in fronto-limbic system biology underlying normal development, aging and disease in the human brain.
journal_name
Brain Resjournal_title
Brain researchauthors
Dalgard CL,Jacobowitz DM,Singh VK,Saleem KS,Ursano RJ,Starr JM,Pollard HBdoi
10.1016/j.brainres.2014.12.031subject
Has Abstractpub_date
2015-03-10 00:00:00pages
42-58eissn
0006-8993issn
1872-6240pii
S0006-8993(14)01719-3journal_volume
1600pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract::Inflammation is an important factor for hypoxia-ischemia (HI) brain injury. Interleukin (IL)-18 is a proinflammatory cytokine which may be a contributor to injury in the immature brain after HI. To investigate the effects of post-HI hypothermia on IL-18 in the developing brain, 7-day-old rats were subjected to left ca...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2006.08.121
更新日期:2006-11-22 00:00:00
abstract::In extracellular recordings from chloral hydrate anesthetized rats the 5-HT3 antagonist, BRL 46470A, failed to prevent or reverse the increase in dopamine cell firing rate produced by systemic or iontophoretically applied morphine. A second 5-HT3 antagonist, tropesitron, was similarly found to be ineffective in antago...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)90665-3
更新日期:1994-02-28 00:00:00
abstract::Fetal mouse retinal ganglion cell axons have been shown to ramify within co-cultured basement membrane secreting tumor explants and upon isolated basement membranes. Here we report that laminin, a glycoprotein found in basement membranes and adhesion sites of a variety of cell types, acted as a substrate for retinal e...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0165-3806(84)90184-6
更新日期:1984-01-01 00:00:00
abstract::In the present study, we assessed the beneficial synergistic effects of concurrent treatment with low doses of cilostazol and probucol against focal cerebral ischemic infarct in rats. The ischemic infarct induced by 2-h occlusion of middle cerebral artery (MCA) and 22-h reperfusion was significantly reduced in rat bra...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2007.04.051
更新日期:2007-07-09 00:00:00
abstract::To achieve a better understanding of the parallel information processing that takes place in the nervous system, many researchers have recently begun to use multielectrode techniques to obtain high spatial- and temporal-resolution recordings of the firing patterns of neural ensembles. Apart from the complexities of ac...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(00)03072-9
更新日期:2000-12-22 00:00:00
abstract::Currently there are no uniform standard treatments for newborn suffering from cerebral hypoxia-ischemia (HI) and to find new and effective strategies for treating the HI injury remains a key direction for future research. Present study was designed to demonstrate that optimal dose (1 or 3%) of creatine monohydrate (Cr...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2014.11.017
更新日期:2015-01-21 00:00:00
abstract::Zinc-enriched (ZEN) neurons are distributed widely throughout the brain and spinal cord. Synaptic vesicle zinc in these neurons is thought to function as a neuromodulator upon its release into the synaptic cleft. Consistent with this possibility, zinc or zinc chelators can alter spatial learning, working memory, and n...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(00)03220-0
更新日期:2001-02-09 00:00:00
abstract::Considerable knowledge exists concerning the events mediating neuron death following a necrotic insult; prompted by this, there have now been successful attempts to use gene therapy approaches to protect neurons from such necrotic injury. In many such studies, however, it is not clear what sequence of cellular events ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(01)02572-0
更新日期:2001-07-20 00:00:00
abstract::Cytochrome P45011B1 (11 beta-hydroxylase) was detected in the brain of male rats by in situ hybridization methods. Normal Sprague-Dawley rats were compared to the transgenic strain TGR(mRen2)27, characterized by the expression of the murine Ren-2d renin gene and the development of severe hypertension. Specific ribopro...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(96)00540-9
更新日期:1996-09-09 00:00:00
abstract::Parkinson's disease (PD) is a common neurodegenerative disease characterized by progressive loss of midbrain dopaminergic neurons. To gain an insight into the mechanisms underlying the progression of PD, gene expression analysis was performed using two different brain regions, the substantia nigra pars compacta (SN) a...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2010.05.066
更新日期:2010-07-30 00:00:00
abstract::The mechanism of the Ca2+-dependent stimulation of neurotransmitter release by 4-aminopyridine in synaptosomes was studied. The stimulation of gamma-[3H]aminobutyric acid and [3H]acetylcholine release by 4-aminopyridine was not significantly affected either by tetrodotoxin or by the absence of Na+ in the medium, where...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(85)91307-1
更新日期:1985-12-30 00:00:00
abstract::While much has been elucidated about the hypothalamic controls of energy balance, the epidemic of obesity continues to escalate. Recent work has suggested that extra-hypothalamic central nervous system structures may play a previously un-appreciated role in the control of ingestive behavior and body weight regulation....
journal_title:Brain research
pub_type: 杂志文章,评审
doi:10.1016/j.brainres.2010.06.009
更新日期:2010-09-02 00:00:00
abstract::The aim of this study was to test the role of the perifornical hypothalamus and adjacent areas in the behavioral and cardiovascular responses to two forms of stress, conditioned fear to context and restraint. Of particular interest was the role of the hypocretin (orexin) containing neurons in these responses. Rats imp...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2006.10.058
更新日期:2007-01-12 00:00:00
abstract::The effect of immunoneutralization of hypothalamic neuropeptide Y (NPY) on food intake was examined to clarify the involvement of NPY in the control mechanism of feeding behavior. Injection of anti-NPY gamma-globulin into the bilateral hypothalamic paraventricular nuclei of 24-h food-deprived rats significantly inhibi...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(93)91727-a
更新日期:1993-01-22 00:00:00
abstract::Basal limbic structures (insula, amygdala, hippocampus and surrounding areas) were explored for unit responses to vagal volleys in awake, sitting, squirrel monkeys. Triple shocks were applied every 4 sec to the left cervical vagus nerve. As a control for adventitious activation by extravagal somatic afferents, units r...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(81)91247-6
更新日期:1981-05-25 00:00:00
abstract::Using the teased fiber recording method, we have compared pathophysiological properties of afferent axons injured in the infraorbital nerve (ION) vs the sciatic nerve in rats. Both myelinated and unmyelinated axons ending in ION neuromas produced much less ongoing discharge than those ending in sciatic nerve neuromas....
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(92)90753-v
更新日期:1992-05-01 00:00:00
abstract::5-HT2 receptor binding sites were measured (by saturation binding of [3H]ketanserin) in brain tissue obtained at postmortem from 19 suicide victims with definite evidence of depression and 19 sex and age-matched control subjects. Five of the suicide victims were receiving antidepressant drugs prior to death; 13 suicid...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(88)91621-6
更新日期:1988-03-08 00:00:00
abstract::The present paper addresses the question of whether a decline of central noradrenergic activity is associated with aging and memory loss in mice. Receptor binding techniques were utilized to compare alpha 2-adrenoceptor density in the brains of aged and young mice. Using [3H]rauwolscine, a selective alpha 2-adrenocept...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(85)91439-8
更新日期:1985-12-16 00:00:00
abstract::Almost anyone who has ever lost weight can attest that it is harder to sustain weight loss than to lose weight. Maintenance of a 10% or greater reduced body weight is accompanied by decreases in energy expenditure to levels significantly below what is predicted solely on the basis of weight and body composition change...
journal_title:Brain research
pub_type: 杂志文章,评审
doi:10.1016/j.brainres.2010.05.062
更新日期:2010-09-02 00:00:00
abstract::The retinohypothalamic tract, a monosynaptic retinal projection to the suprachiasmatic nucleus (SCN), is the path by which light entrains the circadian system to the external photoperiod. Serotonergic neurons in the mesencephalic median raphe nucleus (MnR) also give rise to a major SCN afferent projection. The present...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2005.02.083
更新日期:2005-05-17 00:00:00
abstract::Brain capillaries form a selective interface, the blood-brain barrier (BBB), between the neural parenchyma and the blood. The factors which regulate this interface are poorly understood. Both the iris and retina possess vascular beds that express some BBB characteristics; therefore, they provide attractive models to f...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(93)91323-k
更新日期:1993-12-03 00:00:00
abstract::The localization of calcitonin gene-related peptide (CGRP)-like immunoreactive (CGRPI) structures in the cochlea was examined in the rat using immunocytochemistry. Numerous CGRPI fibers entered the organ of Corti in the intraganglionic spiral bundle and formed a dense fiber patch at the base of the inner hair cells. M...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(85)90992-8
更新日期:1985-12-09 00:00:00
abstract::Adenosine, agmatine and kynurenic acid are endogenous ligands acting on different (e.g. adenosine, NMDA, alpha(2)-adrenergic and imidazoline) receptors with a potential role in nociception at the spinal level. Their antinociceptive effects have already been investigated as monotherapy, but only a few studies have repo...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2004.09.039
更新日期:2004-12-10 00:00:00
abstract::A large body of evidence shows that methamphetamine (METH) causes sustained damage to the brain in animal models and human METH users. In chronic users there are indications of cognitive and motor deficits. Striatal neuropeptides are in a position to modulate the neurochemical effects of METH and consequently striatal...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2013.03.010
更新日期:2013-05-13 00:00:00
abstract::The L-isomer of beta-N-methylamino-L-alanine (BMAA), present in free form in seed of Cycas circinalis, elicits in spinal cord cultures a pattern of acute postsynaptic neuronal vacuolation comparable to that induced by beta-N-oxalylamino-L-alanine (BOAA), an excitotoxic amino acid of greater potency isolated from seed ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(87)90342-8
更新日期:1987-05-05 00:00:00
abstract::Recent reports of the transsynaptic transfer of wheat germ agglutinin (WGA) in several systems are consistent with the hypothesis that WGA binds to endogenous glycoproteins(s) which undergo transsynaptic transfer. Such molecules might be involved in trophic interactions between neurons. Since these hypothetical glycop...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(86)90599-8
更新日期:1986-12-03 00:00:00
abstract::The regulation of circadian rhythms changes with age. In humans, changes in the timing of sleep and wakefulness are especially common. In Syrian hamsters Mesocricetus auratus the free running period of the activity/rest rhythm shortens with age. The present study tested the hypothesis that critical age-related changes...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(95)00423-n
更新日期:1995-07-17 00:00:00
abstract::The effect of the xanthine derivative propentofylline (HWA 285) on metabolic and functional recovery in rabbit spinal cord after 20 and 30 min ischemia and 4 days of reperfusion was investigated. Pre-treatment with 20 mg/kg significantly improved recovery of the energy state in the spinal cord, however, without signif...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(92)91114-t
更新日期:1992-09-11 00:00:00
abstract::Strong evidence obtained from in vivo and ex-vivo studies suggests the existence of interaction between dopaminergic and nitrergic systems. Some of the observations suggest a possible implication of nitric oxide (NO) in dopamine (DA) uptake mechanism. The present work investigated the interaction between both systems ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2007.12.054
更新日期:2008-03-10 00:00:00
abstract::Intraventricular injections of carbachol, a cholinergic agonist, into free-running mice can cause phase dependent phase shifts, in both directions, in the circadian rhythm of wheel-running activity. These effects mimic the action of light in entraining the circadian pacemaker, and suggest that acetylcholine may play a...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(81)90059-7
更新日期:1981-05-11 00:00:00