Abstract:
:The effect of the detergent digitonin on lysis of granule and plasma membranes of human neutrophils was studied. Either linear or sigmoid dose-response for release of the cytoplasmic marker lactate dehydrogenase and the granule markers lysozyme, beta-glucuronidase, lactoferrin, and myeloperoxidase was noted using digitonin concentrations ranging from 0.001 to 0.1 mM. However, release of the cytosol compartment was far more sensitive to the detergent than the granule compartment, with more release of lactate dehydrogenase than of lysozyme at 0.01-0.08 mM digitonin. Distinction between the two compartments was optimal at 0.025 mM digitonin. By examining in parallel the digitonin-induced release of exogenous fluorescent or luminescent indicators, a granule location was demonstrated for the pH indicator 9-aminoacridine, while the calcium probes aequorin and Quin 2 were released coincident with release of the cytosolic enzyme lactate dehydrogenase. These findings were employed to validate use of the indicators for monitoring of ion translocation in the intact cell. The differential effect of this detergent on subcellular membranes provides a broadly applicable technique for rapid assessment of the subcellular localization of tracer substances. Rapid monitoring may help to avoid problems of redistribution during cell fractionation.
journal_name
Tissue Celljournal_title
Tissue & cellauthors
Styrt B,Johnson PC,Klempner MSdoi
10.1016/0040-8166(85)90036-9subject
Has Abstractpub_date
1985-01-01 00:00:00pages
793-800issue
6eissn
0040-8166issn
1532-3072journal_volume
17pub_type
杂志文章相关文献
TISSUE & CELL文献大全abstract::Three systems, two sensory and one protective, are present in the skin of the living Australian lungfish, Neoceratodus forsteri, and in fossil lungfish, and the arrangement and innervation of the sense organs is peculiar to lungfish. Peripheral branches of nerves that innervate the sense organs are slender and unprote...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2016.11.009
更新日期:2017-02-01 00:00:00
abstract::1. To be mechanically effective, supporting structures which are helicoidal need to be monodomain, with planar or concentric layers. 2. To achieve this in cholesteric liquid crystalline chemical models, a constraining surface is required. 3. The prediction which logically follows from this is that natural helicoidal s...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(88)90013-4
更新日期:1988-01-01 00:00:00
abstract::Alkaline phosphatase activity in the intertubular tissue of the testes of the domestic fowl was examined using an ultracytochemical technique based on the lead capture method. In the interstitial tissue, the Leydig cells, transitional cells and the fibroblasts displayed enzyme activity on their cell membranes. Vacuole...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(90)90015-2
更新日期:1990-01-01 00:00:00
abstract::The electrophysiological properties and structure of heart muscle fibres of the lobster myocardium have been investigated. Discontinuities were observed in the expected decrement of electrotonic potentials in a study of the passive membrane properties of the muscle. Injection of Procion yellow revealed transverse barr...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(71)80018-6
更新日期:1971-01-01 00:00:00
abstract::Myosin as well as actin filaments could be seen in negatively stained preparations of fresh chicken gizzard homogenized in buffered KCI (I=0.12, pH 6.85), in a 1 : 1 ratio. Myosin filaments were also present when homogenates were diluted (1 : 9) with solutions containing additional Mg(++) and ATP provided naturally oc...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(69)80007-8
更新日期:1969-01-01 00:00:00
abstract::Chondrocytes can be isolated from human adult cartilage from metatarsal phalangeal joints. After enzymatic digestion to isolate viable cells, confluent monolayers were obtained 2-4 weeks after the start of cell division. Chondrocytes cultures, initiated and maintained in HAM's F12 with bovine fetal serum without the a...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1054/tice.1999.0069
更新日期:1999-12-01 00:00:00
abstract::Epithelial and peritubular cells associated with the reproductive tract of the snail, Limnaea stagnalis, contain an extensive system of endoplasmic reticulum that is often dilated with many closely packed intracisternal tubules. The intracisternal tubules are approximately 24-28 nm in diameter and they are often hexag...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(84)90059-4
更新日期:1984-01-01 00:00:00
abstract::Diabetic nephropathy is the common cause of leading to end stage of renal disease (ESRD). Satureja khozestanica essential oil (SKEO) was used as an antioxidant and antidiabetic for the inhibition of diabetic nephropathy. Forty male rats were uninephrectomized and divided in four groups randomly; group one as control, ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2010.11.004
更新日期:2011-02-01 00:00:00
abstract::Fluorescent staining of Rhodnius prolixus ovarioles with rhodaminylphalloidin revealed an elaborate interconnecting meshwork of F-actin encasing the microtubule rich core. The thick actin struts have branches extending partially into the nurse cell lobes extending from the syncytical core. Basally the network ends at ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(86)90075-3
更新日期:1986-01-01 00:00:00
abstract::In this study, we aimed to investigate the presence of asprosin (ASP) in the liver, kidneys, heart, stomach, testicles and brain and to determine the serum and tissue asprosin levels in diabetic rats. A total of 14 male Wistar Albino rats were divided into two groups, each containing 7 rats: (I) control group and (II)...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2020.101397
更新日期:2020-10-01 00:00:00
abstract::The expression and localization of gap junctional proteins connexin (Cx) 26, 32, and 43 was examined in human dental pulp. Dental pulp tissues were obtained from human third molars immediately after extraction. Some pulp tissues were used for cell culture, and the rest for histological observations. Immunostaining for...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(02)00028-9
更新日期:2002-06-01 00:00:00
abstract::The watery vacuoles first described from larval insect fat body (Chironomus, Voinov, 1927; Aedes, Wigglesworth, 1942; Rhodnius, Wigglesworth, 1967) have been studied in 4th and 5th stage Calpodes larvae. The vacuoles arise at the beginning (E + 6-24 hr) of the 4th stadium from plasma membrane infolds that separate fro...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(83)90039-3
更新日期:1983-01-01 00:00:00
abstract::Parathyroid hormone/parathyroid hormone-related protein receptor (PPR) signaling is known to be involved in tooth development. In bone, extracellular matrix protein osteopontin (OPN) is a negative regulator of PPR signaling in bone formation. However, the role of OPN in modulation of PPR action in tooth development is...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2011.02.003
更新日期:2011-06-01 00:00:00
abstract::Research spanning almost 50 years has highlighted unique characteristics and irreplaceable list of diverse functions performed by peroxisomes in various model systems. Peroxisomes are single membrane bound highly dynamic organelles ubiquitous to most eukaryotic cells. Proliferation by division of pre-existing organell...
journal_title:Tissue & cell
pub_type: 杂志文章,评审
doi:10.1016/j.tice.2017.03.002
更新日期:2017-04-01 00:00:00
abstract::The general identification of endocrine cells in the stomach of the lizard Podarcis hispanica was carried out by their response to the Grimelius and Masson-Fontana techniques. 11 immunoreactive cell-types, positive for chromogranin-, serotonin-, caerulein/gastrin/ cholecystokinin (CAER/G/CCK)-, glucagon-like-peptide-1...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(92)90041-5
更新日期:1992-01-01 00:00:00
abstract::The main purpose of the article is to review recent knowledge about growth factors and their effect on the chondrogenic differentiation of mesenchymal stem cells under in vitro conditions. Damaged or lost articular cartilage leads to progressive debilitation, which have major impact on the life quality of the affected...
journal_title:Tissue & cell
pub_type: 杂志文章,评审
doi:10.1016/j.tice.2011.11.005
更新日期:2012-04-01 00:00:00
abstract::The imaginal antenna of the male silkmoth Antheraea polyphemus is a feather-shaped structure consisting of about 30 flagellomeres, each of which gives off two pairs of side branches. During the pupal stage (lasting for 3 weeks), the antenna develops from a leaf-shaped, flattened epidermal sac ('antennal blade') via tw...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(95)80048-4
更新日期:1995-06-01 00:00:00
abstract::The oocyte cortex undergoes dramatic changes during oogenesis in Rhodnius prolixus. Despite numerous studies examining oogenesis in the telotrophic ovariole, none has investigated the ultrastructural details of the oocyte cortex, in particular, the lateral cortical cytoskeleton. Indirect immunofluorescent staining of ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(93)90081-u
更新日期:1993-06-01 00:00:00
abstract::Lipid bodies (LB), lipid-rich inclusions abundantly present in cells engaged in inflammation, are specialized intracellular domains involved in generating inflammatory mediators, the eicosanoids. Since the acute Trypanosoma cruzi infection triggers a potent inflammatory reaction characterized by a great increase of pe...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(02)00105-2
更新日期:2003-02-01 00:00:00
abstract::The vascular endothelium as well as subendothelium are objects of many researches as it is directly involved in a multiplicity of physiological and pathological settings. Detailed study of endothelial function became feasible with the development of techniques to culture endothelial cells (EC) in vitro. Limitations of...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2015.03.004
更新日期:2015-06-01 00:00:00
abstract::Centrioles are involved in the formation of cilia and flagella and in the organization of the centrosome, the main microtubule-organizing center of the cell. Since structural and functional centriole aberrations can contribute to several human diseases, these organelles have recently focused the attention of cell biol...
journal_title:Tissue & cell
pub_type: 杂志文章,评审
doi:10.1016/j.tice.2010.01.002
更新日期:2010-04-01 00:00:00
abstract::Lithium Chloride (LiCl) has been used as a canonical Wnt pathway activator due to its ability to inhibit a glycogen synthase kinase-3. The aim of the present study was to investigate the effect of LiCl on cell proliferation and osteogenic differentiation in stem cells isolated from human exfoliated deciduous teeth (SH...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2016.08.005
更新日期:2016-10-01 00:00:00
abstract::A culture technique for the in vitro growth of antennal cells from honeybee is described. On the basis of morphological and immunocytochemical criteria, the cultured cells could be classified into neural and non-neural cells. Neural cells (type D) exhibited the main morphological features of insect olfactory receptor ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(94)90007-8
更新日期:1994-08-01 00:00:00
abstract::Degeneration of intervertebral disk (IVD) has been increased in recent years. The lumbar herniation can be cured using conservative and surgical procedures. Surgery is considered after failure of conservative treatment. Partial discectomy, fusion, and total disk replacement (TDR) are also common surgical treatments fo...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2014.05.008
更新日期:2014-10-01 00:00:00
abstract::The incorporation of H(3)-histidine and H(3)-glucosamine by ovarian follicles of cecropia moths during incubation in female blood was followed autoradicgraphically. Labeling was most prominent in the follicle cells, the spaces between these cells, and the nascent yolk spheres in the oocyte cortex. Results of pulse-cha...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(69)80037-6
更新日期:1969-01-01 00:00:00
abstract::The ovoid cells of the branchial heart complex of Sepia officinalis L. were investigated with respect to their role in detoxification processes. The electron microscopical localization of in situ injected ferritin in the endocytotic-lysosomal system, and the fluorescence microscopical localization of protein-bound Eva...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(98)80085-2
更新日期:1998-12-01 00:00:00
abstract::Due to the recent importance assumed by the apoptotic process as a tool in the therapeutic protocols, we investigated the onset and rate of drug-induced apoptosis in a cell model of prostatic tumor: the DU-145 cells. The human prostatic cancer cells, DU-145, have been treated with pro-apoptotic substances like puromyc...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2005.06.006
更新日期:2005-10-01 00:00:00
abstract::Bone substitutes are used in nearly half of all musculoskeletal surgeries. The "gold standard" graft is an autograft that is limited by supply and site morbidity. Therefore, allograft sources are the current alternative for clinical practice with some side effects, such as immune responses and risk of disease transmis...
journal_title:Tissue & cell
pub_type: 杂志文章,评审
doi:10.1016/j.tice.2021.101494
更新日期:2021-01-16 00:00:00
abstract::This paper describes the fine structure of the cockroach subgenual organ, a complex ciliated mechanoreceptor that detects vibrations in the substrate upon which the animal stands. Located beneath the knee in each walking leg, the cockroach subgenual organ is a thin, fan-shaped flap of tissue slung across the dorsal bl...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(75)80009-7
更新日期:1975-01-01 00:00:00
abstract::Antiserum to leucokinin I, a neuropeptide originally isolated from the cockroach Leucophaea maderae, was used for immunocytochemical labeling of neurons in the brain and ventral ganglia of the moth Spodoptera litura during postembryonic development. In the ventral ganglia, leucokinin-like immunoreactivity begins to oc...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(98)80008-6
更新日期:1998-02-01 00:00:00